EMAGE mouse gene expression spatial database

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EMAGE:14678

Syvn1 synovial apoptosis inhibitor 1, synoviolin ( MGI:1921376)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005614. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005614_01	euxassay_005614_02	euxassay_005614_03	euxassay_005614_04

euxassay_005614_05	euxassay_005614_06	euxassay_005614_07	euxassay_005614_08	euxassay_005614_09

euxassay_005614_10	euxassay_005614_11	euxassay_005614_12	euxassay_005614_13	euxassay_005614_14

euxassay_005614_15	euxassay_005614_16	euxassay_005614_17	euxassay_005614_18	euxassay_005614_19

euxassay_005614_20	euxassay_005614_21	euxassay_005614_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14678_wholemount_strong.wlz

14678_wholemount_moderate.wlz

14678_wholemount_weak.wlz

14678_wholemount_possible.wlz

14678_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14678_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 01 02 03 04 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4964
Entity Detected:	Syvn1, synovial apoptosis inhibitor 1, synoviolin ( MGI:1921376)
Sequence:	sense strand is shown >T4964 GTCGTAGCTATCCCTGGAATGAGGCGCTTACACATTTTATTTCTTTCATGCCTGACATAAAGTCTGGCCC TTGCTCGCTCCTGCCCCCCGTCCAAATGGCTCGGCCCGCGGAACGCCCCATCTTCCAGGCACATTGAGAG CCGGAGTCTTGGAGGAGTTTAGGGTGGTGATTCTACAACGGCGACTAGCAAGTGGCGGGCTTCAGCCCTT TCCCGCTGCTCTCCTGGTCGCGACCACACGTCACAGCTCTCGCTCGTTCCGGTTGCTCGCGCACGGGCCC CAGAAGCGCAGGCGAGATCGGAGCGCGCAAAGAGAACTTGGTACGGTCCACTCCGCCGCGCCCCGCGCCG CCGGAAGTGAGGTGTCTTACCCCCGAAGTTCCGGTTCGCAGGGGGTGGGGAGTGTTGTTAACCGGAGCGG CTGCCGCAGTCGCGGTGATTGAGCGTGCTCGCGGCGCTGGGCTCCTGGTCTCTGGGCCAGGGCGATGTTC CGCACCGCAGTGATGATGGCGGCCAGCCTGGCGCTAACCGGGGCAGTGGTGGCTCATGCCTACTACCTCA AACACCAGTTCTACCCCACTGTAGTGTATTTGACCAAGTCCAGCCCCAGCATGGCAGTCCTGTACATCCA GGCCTTTGTCCTTGTCTTCCTCTTGGGCAAGGTGATGGGCAAGGTGTTCTTCGGGCAGCTGAGGGCAGCA GAGATGGAGCACCTTCTGGAACGGTCCTGGTACGCTGTTACTGAGACTTGTTTGGCCTTCACCGTTTTTC GGGATGACTTCAGCCCTCGCTTTGTGGCGCTCTTTACGCTGCTCCTCTTCCTCAAATGTTTCCATTGGTT GGCTGAAGACCGTGTGGACTTTATGGAACGCAGCCCCAACATCTCCTGGCTCTTCCACTGCCGCATCGTC TCTCTCATGTTTCTCCTGGGTATCCTGGACTTCCTCTTCGTCAGCCACGCTTATCACAGCATCCTGACCC GTGGGGCTTCTGTGCAGCTGGTATTTGGCTTTGAGTACGCCATTCTGATGACCATGGTGCTTACCATCTT CATCAAGTATGTGCTGCACTCCGTGGACCTCCAGAGCGAGAACCCCTGGGACAACAAGGCTGTATACATG CTCTACACGGAGCTGTTTACAGGCTTCATCAAGGTCCTGCTGTACATGGCCTTCATGACCATCATGATCA AGGTGCACACATTCCCACTCTTTGCCATTAGGCCCATGTACCTGGCCATGAGGCAGTTCAAGAAAGCTGT GACAGATGCCATCATGTCTCGCCGAGCCATCCGCAACATGAACACACTGTACCCAGATGCCACCCCCGAG GAGCTCCAGGCAGTGGATAATGTCTGTATCATCTGCAGAGAAGAAATGGTGACTGGTGCTAAGAGATTGC CTTGCAACCACATCTTTCACACGAGCTGCCTGCGCTCCTGGTTCCAGAGACAGCAGACCTGCCCGACATG CCGCATGGATGTCCTGCGGGCATCGTTGCCAGCCCAGTCACCACCACCTCCTGAGCCTGCTGACCAAGGA CCACCCCCCGCCCCTCATCCCCAACCGCTGCTGCCACAGCCCCCTAATTTCCCCCAGGGCCTCCTGCCTC CTTTTCCTCCAGGCATGTTCCCACTGTGGCCCCCAATGGGTCCCTTTCCACCTGTCCCGCCTCCCCCAAG CTCAGGAGAGGCTGCGGCCCCTCCACCCACCAGTACAGCCGTTTCTCGGCCTAGTGGAGCAGCCACCACC ACAGCTGCTGGCACCAGTACTTCTGCCCCAGCACCTGGGTCTGTACCTGGCCCAGAGGCTGGTCCTGCCC CCGGCTTCCCTTTCCCTCCTCCTTGGATGGGTATGCCTCTGCCTCCACCTTTTGCCTTCCCCCCAATGCC TGTGCCCCCTGCGGGCTTTGCTGGCCTAACCCCAGAGGAGCTGCGAGCACTAGAGGGCCATGAGCGGCAG CACCTGGAGGCCCGGCTGCAGAGTCTGCGCAACATACACACACTACTGGATGCTGCCATGCTTCAAATCA ACCAGTACCTCACTGTGCTGGCTTCCCTGGGGCCCCCCAGGCCAGCTACTTCAGTGAACCCCACTGAAGA GACTGCCTCTACAGTGGTATCTGCTGCCCCTTCCACCAGCGCCCCCAGCTCTGAGGCTCCTACCCCGTCT CCGGGAGCTTCCCCACCAATTCCTGAAGCAGAAAAGCCTCCTGCTCCTGAGTCAGTGGGCATTGTAGAGG AGCTTCCCGAGGACGGAGAGCCTGATGCTGCAGAACTCCGCCGGCGTCGCCTGCAGAAGCTGGAGTCCCC TGTTGCCCACTGACACTGCCCAGACCTGGCCCTGTTCTCTTGAGTGGCCCTCACTGGAACACGTCCTGCC ATCAAGTGCCAGCTCCCTCTCTGCTTGCACCAGGGAGTAATAGCCCCAGTTGAGAAAGACTTGGCAGGAT CTCTGAGGATCAAGGAGAAGTGTCTGGGCTTCCAGTTGATCCATCCCCAGTGCCCCTGGCAGCCATGGAG ATACTGGTCAGCTCTAACCTCCCTCCACTTCTGCCATGTTCAACTGGGGCCTTCAAAGTAGAAGCTGAAT CTCTGGTAAGCCTTCTCTTCCATGCTTTCTGGGAGAAGGTGAAGCCCCTCCAAGCCCTGCTTGTGAGTAT GGGACCATGCTGCAGTGCCGAACAGTATTAGCTTCTGTTCCCAAGTGTGGAAACCCAGAGGGGCTGAAGA CAGACCAGGACCTTGCCCCACCCTCCTGCCAAGACTGGTACCAGTCTCTTTCCTCTAGCCCAGTCTTCCC AGAACCCCTTTGTGATGGTGGCTGTGCCCCCCGAAGCCCTGTGGCATTTCCATGTCTTACTGGCAACCAC ACAACTCAGGGAAAGGAGTGCCTGGGGGTGGGGCACAGGCGGGCAGCACTGAGGGACCCTGCCCTGCCCC TCCCCAGCTCTTTCCCCATCTCACCCAGCAGCCACTGCCTGGTGGGCCTGGCTAAGGGTGTGTGCTGCTC CTTAAACCACTGCTCCCCAGAACCCAAGGCAGGCCACCTCCAACCTGTGGGATGTCGTCAGGATTGGAAC TATTCTGTACCTACTGGCTTTGGGCTTAAATTTTGTCTTCTGAATTTGAATGCTTGACCCCAGGAAGGAG GAGCAGGTGTGGGGCTAGGTACCTGGACTTCGCAGTTTAGAACAAGCTCTGGGCCGGGCCGGGCCAGGCC AGGCCTAGGGAGCCAAGGCCTAGCTGCTGCTTCCTTCTTTTGGTTTTGTGTTACAGGAGTTTCTGGAGAG TTTCAGATGATTATTTAATTTGTAAATATTGTATAAATTTTAATAGCTTAAATTGTATATACAGCTCAAT AAAAACTTGCATTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5136398 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5136398 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14679 same embryo
	EMAGE:14677 same embryo
	EMAGE:14680 same embryo
	EMAGE:14675 same embryo
	EMAGE:14676 same embryo
	EurExpress:euxassay_005614 same experiment
	MGI:4828578 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source