EMAGE mouse gene expression spatial database

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EMAGE:14682

Nrxn1 neurexin I ( MGI:1096391)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005585. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005585_01	euxassay_005585_02	euxassay_005585_03	euxassay_005585_04

euxassay_005585_05	euxassay_005585_06	euxassay_005585_07	euxassay_005585_08	euxassay_005585_09

euxassay_005585_10	euxassay_005585_11	euxassay_005585_12	euxassay_005585_13	euxassay_005585_14

euxassay_005585_15	euxassay_005585_16	euxassay_005585_17	euxassay_005585_18	euxassay_005585_19

euxassay_005585_20	euxassay_005585_21	euxassay_005585_22	euxassay_005585_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14682_wholemount_strong.wlz

14682_wholemount_moderate.wlz

14682_wholemount_weak.wlz

14682_wholemount_possible.wlz

14682_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14682_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

arm

moderate

regional

moderate expression: see section 01 23

leg

moderate

regional

moderate expression: see section 01 02 03 04 05 19 20 21 22 23

trunk mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 16 17 18 19 20 21 22 23

forebrain

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

hindbrain

strong

homogeneous

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain

strong

homogeneous

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18

facial vii ganglion

strong

regional

strong expression: see section 05 06 18 19 20

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 07 17

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 16 17 18 19 20 21

vagus x ganglion

strong

regional

strong expression: see section 08 16

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 08 16 17 18

trigeminal v nerve

strong

regional

strong expression: see section 09 16

spinal cord

strong

homogeneous

strong expression: see section 07 08 09 10 11 12 13 14

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 09

cervical ganglion

moderate

regional

moderate expression: see section 08

thoracic ganglion

moderate

regional

moderate expression: see section 11 12

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 15 16 moderate expression: see section 10 14

neural retina

strong

regional

strong expression: see section 02 03 04 22 23

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 11 12 14 15 16 17 moderate expression: see section 08 18

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 11 12 14 15

vomeronasal organ

moderate

regional

moderate expression: see section 11 14

oral epithelium

moderate

regional

moderate expression: see section 05 20 weak expression: see section 06 07 08 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5543
Entity Detected:	Nrxn1, neurexin I ( MGI:1096391)
Sequence:	sense strand is shown >T5543 GGAAAACAGACCAACCATCTGCATCTAGACCAGCCTTCCTCTCCCTCGCTCCCTTTTAGAGGAGGTTTTC CTGCCTGGAATCTGGAAAAGAAGAGTATATTGCCACGTTCAAGGGATCTGAATACTTCTGCTACGACTTA TCTCAAAACCCCATTCAAAGCAGCAGTGATGAAATAACTCTGTCCTTTAAAACTCTTCAAAGGAACGGAC TGATGCTTCACACAGGGAAATCAGCTGATTATGTCAATCTTGCATTGAAAAATGGAGCTGTCTCTCTGGT CATTAATTTGGGATCAGGGGCCTTTGAAGCACTAGTGGAGCCTGTGAATGGAAAGTTTAATGATAATGCC TGGCATGATGTGAAAGTCACCAGGAATCTGCGTCAGGTGACAATATCAGTGGATGGAATTCTTACCACAA CGGGCTACACGCAAGAAGATTACACCATGCTGGGGTCTGATGACTTTTTCTATGTTGGAGGCAGCCCCAG CACAGCCGACCTTCCAGGGTCACCAGTCAGTAACAACTTTATGGGCTGTCTCAAAGAGGTTGTATATAAA AATAATGATGTAAGGCTGGAATTATCGCGACTTGCCAAGCAAGGAGATCCTAAGATGAAGATTCACGGTG TGGTGGCTTTTAAGTGTGAAAATGTGGCAACCTTAGACCCAATAACTTTTGAGACCCCGGAATCTTTCAT TTCTTTGCCTAAGTGGAATGCAAAGAAAACAGGCTCAATATCATTTGATTTCCGTACAACGGAGCCAAAT GGCCTCATCCTGTTTAGCCATGGCAAGCCAAGACATCAGAAGGATGCCAAGCACCCACAGATGATTAAGG TTGACTTTTTTGCTATTGAGATGCTGGATGGCCACTTGTACCTCCTCTTAGACATGGGATCAGGTACTAT AAAAATCAAAGCCCTGCAGAAGAAAGTCAATGATGGAGAATGGTATCACGTGGATTTCCAACGGGATGGA CGGTCAGGTACCATTTCTGTCAACACACTACGCACTCCCTACACAGCTCCCGGTGAGAGTGAGATCCTGG ACCTAGATGATGAGTTGTACCTGGGTGGCTTGCCAGAAAATAAGGCTGGCCTAGTCTTCCCTACCGAGGT GTGGACTGCTCTGCTCAACTACGGCTACGTGGGCTGCATCCGGGACCTATTTATTGATGGCCAGAGCAAA GACATCCGGCAGATGGCGGAAATTCAGAGCACTGCTGGAGTGAAGCCATCCTGCTCAAAAGAGACAGCAA AACCGTGCCTTAGCAATCCTTGCAAAAACAATGGCATGTGCAGAGATGGCTGGAACAGATACGTCTGCGA TTGCTCCGGAACAGGCTATCTCGGCAGGTCCTGTGAGAGAGAGGCAACGGTTTTGAGCTACGACGGGAGC ATGTTCATGAAAATCCAGCTTCCGGTGGTGATGCACACTGAGGCTGAGGACGTGTCCTTGCGGTTCCGAT CCCAGCGTGCATATGGTATCCTGATGGCGACCACTTCTAGAGACTCTGCAGACACCCTTCGATTGGAGCT AGATGCAGGACGTGTGAAACTCACGGTCAATCTAGATTGTATCAGGATTAACTGTAATTCCAGCAAAGGT CCTGAGACCCTTTTTGCTGGCTATAACCTCAATGATAATGAATGGCACACAGTGCGTGTGGTTCGGCGAG GAAAAAGTTTAAAGCTAACCGTGGATGATCAACAAGCCATGACAGGCCAAATGGCAGGTGATCATACAAG GCTGGAGTTTCATAACATAGAGACAGGCATCATCACCGAACGACGCTACCTTTCTTCTGTCCCCTCCAAT TTCATTGGGCACCTGCAAAGCCTGACATTTAATGGAATGGCATACATAGACTTGTGCAAAAATGGTGATA TAGATTACTGTGAACTCAATGCCAGGTTTGGATTCAGGAACATCATCGCAGATCCTGTCACCTTCAAGAC CAAATCGAGCTATGTTGCCTTAGCTACATTGCAAGCCTACACCTCTATGCATCTATTTTTCCAATTCAAG ACAACATCTCTAGATGGACTAATTCTGTATAACAGTGGGGATGGAAATGACTTTATTGTGGTTGAATTAG TTAAAGGGTACTTACATTATGTGTTTGACTTGGGAAACGGTGCTAACCTCATCAAAGGGAGCTCAAACAA ACCACTCAACGACAATCAGTGGCACAATGTGATGATTTCACGGGACACCAGCAATCTCCACACAGTAAAG ATCGACACAAAAATCACAACACAAATCACTGCGGGAGCCAGAAATCTAGACCTCAAGAGTGACTTGTATA TTGGAGGAGTGGCTAAAGAAACATATAAATCCCTGCCAAAACTGGTCCATGCCAAAGAAGGCTTTCAAGG TTGCCTGGCATCTGTGGACTTGAATGGCAGACTTCCAGATCTCATCTCGGATGCTCTCTTCTGCAATGGG CAAATTGAGAGAGGATGCGAAGGGCCCAGCACAACCTGCCAAGAGGATTCATGTTCAAATCAAGGCGTTT GCTTGCAGCAATGGGATGGCTTCAGCTGTGACTGTAGCATGACTTCCTTCAGTGGCCCACTCTGCAATGA CCCTGGGACAACATATATCTTTAGCAAAGGTGGTGGACAGATTACATATAAGTGGCCTCCCAATGACCGC CCCAGTACACGAGCAGACAGGCTGGCCATCGGATTTAGCACTGTCCAGAAGGAAGCCGTGTTGGTGCGTG TGGACAGTTCCTCAGGACTGGGTGACTACCTGGAGCTGCACATACACCAAGGAAAAATTGGAGTTAAGTT TAATGTTGGGACAGATGACATCGCCATCGAAGAGTCTAATGCAATCATTAATGATGGGAAATACCATGTA GTACGTTTCACAAGGAGTGGTGGCAATGCCACGTTACAGGTGGACAGCTGGCCAGTTATCGAACGCTACC CTGCAGGAAACAATGATAACGAGCGCCTGGCGATTGCTAGACAGCGAATTCCATATCGACTTGGTCGAGT AGTTGATGAATGGCTACTCGACAAAGGGCGTCAGCTCACAATCTTCAATAGCCAAGCAACCATAATAATT GGCGGGAAAGAGCAGGGCCAGCCCTTCCAGGGCCAGCTCTCTGGTCTTTACTACAATGGCTTGAAAGTTC TGAATATGGCAGCAGAGAACGATGCCAACATCGCCATAGTGGGGAATGTGAGGCTGGTCGGTGAAGTGCC TTCCTCTATGACAACTGAGTCGACAGCCACTGCCATGCAGTCTGAGATGTCCACCTCAATCATGGAGACC ACCACAACCCTGGCTACCAGCACAGCTAGAAGAGGCAAGCCCCCCACAAAGGAACCCATCAGCCAGACCA CAGATGACATCCTTGTGGCCTCGGCAGAGTGTCCCAGTGACGATGAGGACATTGACCCCTGTGAGCCGAG CTCAGCCAACCCCACCAGAGTAGGTGGCCGTGAACCATACCCAGGCTCTGCAGAGGTGATTCGGGAGTCC AGCAGTACCACTGGCATGGTGGTGGGGATTGTCGCAGCAGCCGCTCTGTGCATCCTCATCCTCCTCTATG CCATGTACAAGTACAGGAACCGGGATGAAGGGTCGTACCACGTGGATGAGAGTCGAAACTACATCAGTAA CTCAGCACAGTCCAATGGGGCTGTGGTCAAGGAGAAGCAACCCAGCAGTGCTAAAAGCGCCAACAAAAAC AAGAAAAACAAGGATAAGGAGTATTACGTCTGATATCAAGATTTGAAACGGACACTTGTATAGAAATAGT CTTCATTTTATCTGAGACATAATATAAACTTTTACTTTCCTTTTTATGAAGCACATACAAAAGAAGACAG GGAATGCGATCAGGAGGGAAAACTCTTTTTAAAAAAAAAAACAAACAAGTATCTCATGCTCTTGTTTCTC AAAAGAAAAAAAAAACATACACAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6466916 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6466916 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14683 same embryo
	EMAGE:14681 same embryo
	EMAGE:14686 same embryo
	EMAGE:14685 same embryo
	EMAGE:14684 same embryo
	EurExpress:euxassay_005585 same experiment
	MGI:4826800 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source