EMAGE mouse gene expression spatial database

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EMAGE:14715

Eps15 epidermal growth factor receptor pathway substrate 15 ( MGI:104583)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005655. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005655_01	euxassay_005655_02	euxassay_005655_03	euxassay_005655_04

euxassay_005655_05	euxassay_005655_06	euxassay_005655_07	euxassay_005655_08	euxassay_005655_09

euxassay_005655_10	euxassay_005655_11	euxassay_005655_12	euxassay_005655_13	euxassay_005655_14

euxassay_005655_15	euxassay_005655_16	euxassay_005655_17	euxassay_005655_18	euxassay_005655_19

euxassay_005655_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14715_wholemount_strong.wlz

14715_wholemount_moderate.wlz

14715_wholemount_weak.wlz

14715_wholemount_possible.wlz

14715_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14715_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 09 10 11 12

telencephalon ventricular layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11

metencephalon rest of alar plate ventricular layer

moderate

regional

moderate expression: see section 01 02 03 04 05 08 09 10 11 12 13 14 15

pons ventricular layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15

midbrain ventricular layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11

facial vii ganglion

strong

regional

strong expression: see section 01

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 02 03 04 11 12

trigeminal v ganglion

strong

regional

strong expression: see section 01 moderate expression: see section 02 03 04 05 06 07 14 15 16 17 18

vagus x ganglion

moderate

regional

moderate expression: see section 04 11 12

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 03 04 05 12 13

dorsal root ganglion

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T952
Entity Detected:	Eps15, epidermal growth factor receptor pathway substrate 15 ( MGI:104583)
Sequence:	sense strand is shown >T952 CCTCGAGNCTGTTGGCCTACTGGGTGCATGATGGAAACACCATGGCTGCGGCAGCCCAGCTCTCCCTGAC ACAGTTGTCAAGTGGGAATCCTGTATATGAAAAATACTACAGACAGGTTGAGGCAGGCAATACTGGAAGG GTGTTGGCGTTAGATGCTGCTGCATTCCTGAAAAAGTCAGGGCTTCCAGACTTGATTCTTGGAAAGATTT GGGATTTAGCTGACACAGATGGCAAAGGTGTCCTGAGCAAACAAGAATTCTTTGTTGCTTTACGGCTTGT GGCATGTGCTCAGAATGGACTGGAAGTTTCACTGAGTAGCCTAAGTCTGGCTGTTCCTCCACCAAGATTT CATGACTCCAGCAGTCCGTTGCTAACCAGTGGGCCCTCAGTTGCTGAGCTCCCGTGGGCTGTAAAGTCTG AAGATAAAGCCAAATATGATGCAATTTTTGACAGTTTAAGCCCAGTGGATGGATTTTTGTCTGGTGATAA AGTGAAACCAGTGTTGCTCAACTCTAAGTTACCTGTGGAAATCCTTGGAAGAGTTTGGGAGTTGAG
Notes:	The probe template was PCR amplified from IMAGE:1970244 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1970244 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14711 same embryo
	EMAGE:14712 same embryo
	EMAGE:14713 same embryo
	EMAGE:14714 same embryo
	EurExpress:euxassay_005655 same experiment
	MGI:4824601 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source