EMAGE mouse gene expression spatial database

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EMAGE:14823

Satb2 special AT-rich sequence binding protein 2 ( MGI:2679336)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005801. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005801_01	euxassay_005801_02	euxassay_005801_03	euxassay_005801_04

euxassay_005801_05	euxassay_005801_06	euxassay_005801_07	euxassay_005801_08	euxassay_005801_09

euxassay_005801_10	euxassay_005801_11	euxassay_005801_12	euxassay_005801_13	euxassay_005801_14

euxassay_005801_15	euxassay_005801_16	euxassay_005801_17	euxassay_005801_18	euxassay_005801_19

euxassay_005801_20	euxassay_005801_21	euxassay_005801_22	euxassay_005801_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14823_wholemount_strong.wlz

14823_wholemount_moderate.wlz

14823_wholemount_weak.wlz

14823_wholemount_possible.wlz

14823_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14823_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 01 02 03 04 05 06 15 16 17 18 19 20 21

elbow mesenchyme

strong

regional

strong expression: see section 01 02

shoulder

strong

regional

strong expression: see section 01 02 03

humerus

strong

regional

strong expression: see section 01 02 03 21 22 23

knee mesenchyme

strong

regional

strong expression: see section 03 04

femur

strong

regional

strong expression: see section 04 05

hypothalamus mantle layer

moderate

single cell

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 weak expression: see section 08

cerebral cortex mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 20 21 22 23 moderate expression: see section 08 09 10 11 16 17 18 19 weak expression: see section 12 15

tongue mesenchyme

moderate

homogeneous

moderate expression: see section 15 16 17 weak expression: see section 10 11 12 13 14

mandible

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 15 16 17 18 19 20 21 22

maxilla

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 16 17 18 19 20 21 22

laryngeal cartilage

moderate

homogeneous

moderate expression: see section 11 12 13

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 05 06 22 23

clavicle

strong

regional

strong expression: see section 06 07 08 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35531
Entity Detected:	Satb2, special AT-rich sequence binding protein 2 ( MGI:2679336)
Sequence:	sense strand is shown >T35531 GCACACAGGGATTATTGTCAGAGATACTGCGTAAGGAAGAAGATCCCAGGACTGCGTCTCAGTCTCTTCT AGTAAACCTGAGGGCCATGCAGAACTTCCTCAACCTGCCTGAAGTGGAGCGTGATCGCATTTACCAGGAT GAGCGAGAGAGGAGCATGAACCCCAATGTGAGCATGGTCTCCTCTGCCTCTAGCAGTCCCAGCTCCTCCC GAACCCCACAGGCCAAAACCTCGACACCGACAACAGACCTCCCTATTAAGGTGGACGGCGCCAACGTCAA CATCACAGCTGCCATTTATGACGAGATCCAACAGGAGATGAAAAGAGCCAAGGTGTCTCAAGCCCTGTTT GCCAAAGTGGCTGCAAACAAAAGTCAGGGCTGGCTTTGCGAACTGCTTCGTTGGAAGGAGAACCCCAGCC CAGAAAACCGCACCCTTTGGGAGAATCTCTGCACCATCCGCCGTTTCCTGAATCTTCCCCAACATGAGCG GGATGTGATCTATGAGGAAGAATCTCGACATCACCACAGTGAACGCATGCAGCATGTGGTCCAGCTCCCA CCTGAGCCCGTGCAGGTCCTTCATCGACAGCAGTCCCAGCCAACTAAGGAGAGCTCCCCTCCCAGAGAAG AAGCACCCCCACCGCCTCCTCCAACAGAAGACAGCTGTGCCAAAAAGCCTCGGTCTCGCACAAAGATCTC TTTGGAAGCCCTGGGCATCCTTCAAAGCTTCATCCATGATGTAGGCCTCTATCCCGACCAGGAAGCCATC CACACACTCTCCGCCCAGCTGGATCTCCCCAAACACACCATCATCAAGT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 92534. Forward Primer - name:092534_F_cDNA_Satb2, sequence:GCACACAGGGATTATTGTCAGA; Reverse Primer - name:092534_N_SP6_cDNA_Satb2, sequence:ACTTGATGATGGTGTGTTTGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14825 same embryo
	EMAGE:14821 same embryo
	EMAGE:14820 same embryo
	EMAGE:14822 same embryo
	EMAGE:14824 same embryo
	EurExpress:euxassay_005801 same experiment
	MGI:4827877 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source