EMAGE mouse gene expression spatial database

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EMAGE:15252

Dtna dystrobrevin alpha ( MGI:106039)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010455. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010455_01	euxassay_010455_02	euxassay_010455_03	euxassay_010455_04

euxassay_010455_05	euxassay_010455_06	euxassay_010455_07	euxassay_010455_08	euxassay_010455_09

euxassay_010455_10	euxassay_010455_11	euxassay_010455_12	euxassay_010455_13	euxassay_010455_14

euxassay_010455_15	euxassay_010455_16	euxassay_010455_17	euxassay_010455_18	euxassay_010455_19

euxassay_010455_20	euxassay_010455_21	euxassay_010455_22	euxassay_010455_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15252_wholemount_strong.wlz

15252_wholemount_moderate.wlz

15252_wholemount_weak.wlz

15252_wholemount_possible.wlz

15252_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15252_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 09 10 11 12 13

pons ventricular layer

moderate

regional

moderate expression: see section 07 08 09 10 13 15 weak expression: see section 14 16

facial vii ganglion

moderate

regional

moderate expression: see section 03

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 16 18 19 20 21 weak expression: see section 17

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 05 06

ventral grey horn

moderate

regional

moderate expression: see section 11 weak expression: see section 09 10 12 13

spinal cord ventricular layer

moderate

regional

moderate expression: see section 10 11

dorsal root ganglion

moderate

regional

moderate expression: see section 07 14 15 weak expression: see section 08 09 10 13

inner ear vestibular component

moderate

regional

moderate expression: see section 02 03 04 19 weak expression: see section 20

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 14 15 16

stomach

weak

regional

weak expression: see section 03 04 05 06 07 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30207
Entity Detected:	Dtna, dystrobrevin alpha ( MGI:106039)
Sequence:	sense strand is shown >T30207 CATGTTCCCAGACCAGCCTGAAAAGCCACTCAACTTGGCTCACATCGTGCCTCCGAGACCTGTAACCAGC ATGAACGACACACTCTTTTCCCACTCTGTTCCCTCCTCAGGAAGTCCTTTCATCACCAGGAGTATGCTTG AGAGTTCAAACCGGCTTGATGAAGAACACAGGCTGATCGCCAGGTACGCCGCACGACTGGCAGCAGAATC CTCCTCATCACAGCCCACACAGCAGAGGAGTGCCCCTGACATCTCCTTCACCATCGATGCAAATAAACAG CAAAGGCAGCTGATTGCAGAGCTAGAGAACAAGAACAGAGAAATCTTACAAGAGATTCAGAGACTTCGGG TAGAACATGAGCAAGCTTCCCAGCCCACGCCAGAGAAAGCTCAGCAGAACCCAACCTTGCTGGCAGAACT CCGGCTCCTCAGACAGCGAAAGGATGAGCTAGAACAGAGAATGTCTGCTCTCCAGGAGAGCCGGAGAGAG CTGATGGTCCAGTTGGAAGGTCTCATGAAGCTCCTGAAGACCCAGGGGGCAAGCTCCCCACGCTCTTCCC CCAGCCACACCATCAGCAGGCCGATCCCCATGCCCATCCGTTCAGCATCTGCCTGTCCCA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4505583), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 9344. Forward Primer - name:009344_F_IRAV51-54_K15_Dtna, sequence:CATGTTCCCAGACCAGCC; Reverse Primer - name:009344_R_SP6_IRAV51-54_K15_Dtna, sequence:GTGGGACAGGCAGATGCT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15254 same embryo
	EMAGE:15257 same embryo
	EMAGE:15256 same embryo
	EMAGE:15253 same embryo
	EMAGE:15255 same embryo
	EurExpress:euxassay_010455 same experiment
	MGI:4824407 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source