EMAGE mouse gene expression spatial database

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EMAGE:15257

Grin1 glutamate receptor, ionotropic, NMDA1 (zeta 1) ( MGI:95819)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010443. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010443_01	euxassay_010443_02	euxassay_010443_03	euxassay_010443_04

euxassay_010443_05	euxassay_010443_06	euxassay_010443_07	euxassay_010443_08	euxassay_010443_09

euxassay_010443_10	euxassay_010443_11	euxassay_010443_12	euxassay_010443_13	euxassay_010443_14

euxassay_010443_15	euxassay_010443_16	euxassay_010443_17	euxassay_010443_18	euxassay_010443_19

euxassay_010443_20	euxassay_010443_21	euxassay_010443_22	euxassay_010443_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15257_wholemount_strong.wlz

15257_wholemount_moderate.wlz

15257_wholemount_weak.wlz

15257_wholemount_possible.wlz

15257_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15257_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

moderate

regional

moderate expression: see section 08 09 10 11 14 15 16 17

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17

telencephalon mantle layer

moderate

regional

moderate expression: see section 02 03 04 09 10 11 14 15 18 20 21 22 23 weak expression: see section 05 06 07 08 19

olfactory cortex mantle layer

moderate

regional

moderate expression: see section 09 10 11 14 15 16 17

medulla oblongata alar plate mantle layer

moderate

regional

moderate expression: see section 07 08 10 11 12 13 14 15 16

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16

rest of cerebellum mantle layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 13 14 15 16 17 18 weak expression: see section 19

pons mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

midbrain mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 13 14 15 16 17 18

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 06 18 19 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 16 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 16 17 18 19 20 21

spinal cord mantle layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14

dorsal root ganglion

weak

regional

weak expression: see section 07 08 09 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30203
Entity Detected:	Grin1, glutamate receptor, ionotropic, NMDA1 (zeta 1) ( MGI:95819)
Sequence:	sense strand is shown >T30203 ACGGGGCCTAATGACACATCCCCAGGAAGCCCACGTCACACAGTGCCCCAGTGCTGTTATGGCTTCTGCG TTGACCTGCTCATCAAGCTGGCACGGACCATGAATTTTACCTACGAGGTGCACCTTGTGGCAGATGGCAA GTTTGGCACACAGGAGCGGGTAAACAACAGCAACAAAAAGGAGTGGAACGGAATGATGGGAGAGCTGCTC AGTGGTCAAGCAGACATGATCGTGGCTCCACTGACCATTAACAATGAGCGTGCGCAGTACATAGAGTTCT CCAAGCCCTTCAAGTACCAGGGCCTGACCATTCTGGTCAAGAAGGAGATCCCTCGGAGCACACTGGACTC ATTCATGCAGCCCTTTCAGAGCACACTGTGGCTGCTGGTGGGGCTGTCAGTTCATGTGGTGGCCGTGATG CTGTACCTGCTGGACCGCTTCAGTCCCTTTGGCCGATTTAAGGTGAACAGCGAGGAGGAGGAGGAGGATG CACTGACCCTGTCCTCTGCCATGTGGTTTTCCTGGGGCGTCCTGCTCAACTCTGGCATTGGGGAAGGTGC CCCCCGGAGTTTCTCTGCTCGTATCCTAGGCATGGTGTGGGCTGGTTTTGCCATGATCATCGTGGCTTCC TACACTGCCAACCTGGCAGCCTTCCTGGTGCTGGATAGGCCTGAGGAGCGCATCACAGGCATCAATGACC CCAGGCTCAGAAACCCCTCAGACAAGTTCATCTATGCAACTGTAAAACAGAGCTCTGTGGATATCTACTT CCGGAGGCAGGTGGAGTTGAGCACCATGTACCGGCACATGGAGAAGCACAATTATGAGAGTGCAGCTGAG GCCA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4507986), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 20135. Forward Primer - name:020135_F_IRAV51-54_E01_Grin1, sequence:ACGGGGCCTAATGACACA; Reverse Primer - name:020135_R_SP6_IRAV51-54_E01_Grin1, sequence:ATGGCCTCAGCTGCACTC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15254 same embryo
	EMAGE:15256 same embryo
	EMAGE:15253 same embryo
	EMAGE:15255 same embryo
	EMAGE:15252 same embryo
	EurExpress:euxassay_010443 same experiment
	MGI:4825232 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source