EMAGE mouse gene expression spatial database

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EMAGE:15264

Nde1 nuclear distribution gene E homolog 1 (A nidulans) ( MGI:1914453)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010375. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010375_01	euxassay_010375_02	euxassay_010375_03	euxassay_010375_04

euxassay_010375_05	euxassay_010375_06	euxassay_010375_07	euxassay_010375_08	euxassay_010375_09

euxassay_010375_10	euxassay_010375_11	euxassay_010375_12	euxassay_010375_13	euxassay_010375_14

euxassay_010375_15	euxassay_010375_16	euxassay_010375_17	euxassay_010375_18	euxassay_010375_19

euxassay_010375_20	euxassay_010375_21	euxassay_010375_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15264_wholemount_strong.wlz

15264_wholemount_moderate.wlz

15264_wholemount_weak.wlz

15264_wholemount_possible.wlz

15264_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15264_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

body cavity or lining

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

limb

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

gland

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

visceral organ system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

integumental system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 10 11 12 13

telencephalon ventricular layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 moderate expression: see section 12 13 14 15 16 17 18 19 20

midbrain ventricular layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 moderate expression: see section 12

sensory organ system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

cardiovascular system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

skeleton

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

tail

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30230
Entity Detected:	Nde1, nuclear distribution gene E homolog 1 (A nidulans) ( MGI:1914453)
Sequence:	sense strand is shown >T30230 AGGAACCGGGACCTCTTGTCAGAGAATAACCGCCTTCGCATGGAGCTGGAGTCTGTGAAGGAGAAGTTTG AGATGCAGCACTCAGAGGGTTACCGGCAGATCTCAGCCTTGGAGGATGACCTGGCGCAGACGAAAGCCAT TAAAGACCAACTGCAGAAATACATTAGGGAACTGGAACAAGCCAATGATGACCTGGAAAGAGCCAAACGA GCCACAATCATGTCCCTGGAAGACTTTGAGCAGCGTTTGAATCAAGCCATTGAAAGAAATGCCTTCCTAG AGAGTGAGCTGGATGAGAAGGAGAATCTTCTAGAATCTGTGCAGAGGCTGAAGGATGAAGCCCGAGATCT GCGGCAGGAATTGGCTGTGCAACAGAAGCAAGACAAGCCCCGGACACCCATGCCAGGCTCAGGGCAAGCC AAGAGGACAGACATGGCTGTGCAGGCCACAGGCTCTGTACCGTCTACTCCAGTAGCTCACCGAGGACCTA GCTCTGGTTTGAACACACCAGGAATGTTCAGACGTGGTCTGGACAGCTCCACTAGTGGGACGCCACTCAC ACCTGCAGCCCGGATATCAGCCCTAAACATCGTTGGGGACCTGCTTCGGAAAGTTGGGGCCCTGGAGTCC AAGCTAGCATCATGCAGGAACTTCATGTATGATCAGTCCCCAAGCCGGACAAGTGGGCCAGCCTCAGGAC GAGGAACCAAAAACAGAGATGGTGTTGACAGAAGGCCAGGCAGTACCAGTGTGGGCGATAAAGGGTCAGG AAAACGCTTGGAGTTTGGGAAGCCAGCCTCAGAGCCAGCATCACCAGCGCTAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3672565), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 9584. Forward Primer - name:009584_F_IRAV59-62_G19_Nde1, sequence:AGGAACCGGGACCTCTTG; Reverse Primer - name:009584_R_SP6_IRAV59-62_G19_Nde1, sequence:GGTAGCGCTGGTGATGCT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15267 same embryo
	EMAGE:15265 same embryo
	EMAGE:15268 same embryo
	EMAGE:15266 same embryo
	EMAGE:15269 same embryo
	EurExpress:euxassay_010375 same experiment
	MGI:4826626 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source