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EMAGE:15358

Rap1gap Rap1 GTPase-activating protein ( MGI:109338)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15358 EMAGE:15358 EMAGE:15358 EMAGE:15358 EMAGE:15358
"Pseudo-wholemount" of euxassay_010532. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010532_01 euxassay_010532_02 euxassay_010532_03 euxassay_010532_04
EMAGE:15358 EMAGE:15358 EMAGE:15358 EMAGE:15358 EMAGE:15358
euxassay_010532_05 euxassay_010532_06 euxassay_010532_07 euxassay_010532_08 euxassay_010532_09
EMAGE:15358 EMAGE:15358 EMAGE:15358 EMAGE:15358 EMAGE:15358
euxassay_010532_10 euxassay_010532_11 euxassay_010532_12 euxassay_010532_13 euxassay_010532_14
EMAGE:15358 EMAGE:15358 EMAGE:15358 EMAGE:15358 EMAGE:15358
euxassay_010532_15 euxassay_010532_16 euxassay_010532_17 euxassay_010532_18 euxassay_010532_19
EMAGE:15358 EMAGE:15358 EMAGE:15358 EMAGE:15358
euxassay_010532_20 euxassay_010532_21 euxassay_010532_22 euxassay_010532_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15358Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15358_wholemount_strong.wlz
15358_wholemount_moderate.wlz
15358_wholemount_weak.wlz
15358_wholemount_possible.wlz
15358_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15358_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09 16 17 18 19 20 21 22 23
radius
weak weak
regionalweak expression: see section 23
ulna
weak weak
regionalweak expression: see section 23
humerus
weak weak
regionalweak expression: see section 02 03 19 20 21 22 23
fibula
weak weak
regionalweak expression: see section 02 03 04 05
tibia
weak weak
regionalweak expression: see section 02 03 04
femur
weak weak
regionalweak expression: see section 04 05 06 07 08 09 19 20 21 22 23
pancreas
moderate moderate
regionalmoderate expression: see section 09 10 11 14 weak expression: see section 15 16
brain
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 weak expression: see section 02 03 04 05 06 07 14 15 16 17 18 19 20 21 22 23
facial vii ganglion
strong strong
regionalstrong expression: see section 05 18 19 20 moderate expression: see section 03 04 06
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 16 moderate expression: see section 05 06
trigeminal v ganglion
strong strong
regionalstrong expression: see section 05 16 17 18 19 20 21 moderate expression: see section 02 03 04 06 07
vagus x ganglion
strong strong
regionalstrong expression: see section 16 moderate expression: see section 06
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 05
spinal cord
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 weak expression: see section 07 14 15 16 17
dorsal root ganglion
strong strong
regionalstrong expression: see section 05 06 07 moderate expression: see section 08 09 10 11 12 13 14 15 16
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 10 11 14 15 weak expression: see section 09 16
viscerocranium
weak weak
regionalExpression in the turbinate bone.
midgut
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 13 weak expression: see section 11 12 14 15
mandible
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17
maxilla
weak weak
regionalweak expression: see section 07 08 09 13 15 16 17 18
kidney calyx
moderate moderate
regionalmoderate expression: see section 09 10 11 16
kidney pelvis
moderate moderate
regionalmoderate expression: see section 08
clavicle
weak weak
regionalweak expression: see section 04 05 06 14 15 16 17 18
scapula
weak weak
regionalweak expression: see section 02 03 19 20
pelvic girdle skeleton
weak weak
regionalweak expression: see section 10 11 17 18
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30570
Entity Detected:Rap1gap, Rap1 GTPase-activating protein ( MGI:109338)
Sequence:sense strand is shown

>T30570
TGCATGCCTATGTGGTGGTGCAGGCAGAGGGGGGTGGCCCCGATGGCCCCCTCTACAAGGTCTCTGTCAC
TGCTAGAGACGATGTGCCCTTTTTTGGGCCTCCCCTCCCGGATCCTGCTGTGTTTAGGAAGGGGCCTGAG
TTCCAGGAATTTCTGCTGACGAAGTTGATCAATGCCGAGTATGCGTGTTACAAAGCAGAGAAGTTTGCCA
AACTGGAGGAGCGGACGCGTGCCGCCCTTCTTGAGACGCTCTACGAGGAGCTCCATATTCACAGCCAGTC
CATGATGGGCCTCGGCGGCGACGACGACAAGATGGAGAACGGCAGCGGTGGGGGTGGCTTCTTCGAGTCT
TTCAAGAGGGTCATCCGGAGCCGGAGTCAGTCCATGGATGCCATGGGACTGAGCAACAAGAAACCCAACA
CCGTGTCAACTAGCCACAGTGGGAGCTTCACCCCCAACAACCCTGACCTGGCCAAGGCAGCCGGCATATC
CCTGATTGTCCCTGGAAAAAGCCCCACAAGGAAGAAGTCTGGTCCGTTTGGCTCACGCCGCAGCAGTGCC
ATTGGCATCGAGAACATCCAGGAGGTTCAGGAGAAGAGGGAGAGCCCTCCAGCTGGCCAGAAGACCCCAG
ACAGCGGGCATGTCTCACAAGAGCCCAAGTCAGAGAACTCATCCACTCAGAGCTCCCCAGAGATGCCCAC
GACCAAGAACAGAGTGGAGTCGGCTGCCCAGAGAACGGAAGTGCTGCAGGGCTTCTCCAGATCTTCCTCC
AGCGCCAGCAGCTTCACCAGCGTGGTGGAGGAGACGGAGGGCGTAGACGGGGATGATACAGGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5708181), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59114. Forward Primer - name:059114_F_IRAV112_e04_Rap1ga1, sequence:TGCATGCCTATGTGGTGG; Reverse Primer - name:059114_R_SP6_IRAV112_e04_Rap1ga1, sequence:GGCCTGTATCATCCCCGT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15360 same embryo
 EMAGE:15355 same embryo
 EMAGE:15357 same embryo
 EMAGE:15356 same embryo
 EMAGE:15359 same embryo
 EurExpress:euxassay_010532 same experiment
 MGI:4827607 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS