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EMAGE:15395

Rab25 RAB25, member RAS oncogene family ( MGI:1858203)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15395 EMAGE:15395 EMAGE:15395 EMAGE:15395 EMAGE:15395
"Pseudo-wholemount" of euxassay_010599. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010599_01 euxassay_010599_02 euxassay_010599_03 euxassay_010599_04
EMAGE:15395 EMAGE:15395 EMAGE:15395 EMAGE:15395 EMAGE:15395
euxassay_010599_05 euxassay_010599_06 euxassay_010599_07 euxassay_010599_08 euxassay_010599_09
EMAGE:15395 EMAGE:15395 EMAGE:15395 EMAGE:15395 EMAGE:15395
euxassay_010599_10 euxassay_010599_11 euxassay_010599_12 euxassay_010599_13 euxassay_010599_14
EMAGE:15395 EMAGE:15395 EMAGE:15395 EMAGE:15395 EMAGE:15395
euxassay_010599_15 euxassay_010599_16 euxassay_010599_17 euxassay_010599_18 euxassay_010599_19
EMAGE:15395 EMAGE:15395
euxassay_010599_20 euxassay_010599_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15395Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15395_wholemount_strong.wlz
15395_wholemount_moderate.wlz
15395_wholemount_weak.wlz
15395_wholemount_possible.wlz
15395_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15395_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
submandibular gland primordium
weak weak
regionalweak expression: see section 06 07 08 16
pancreas
weak weak
regionalweak expression: see section 08 09 10
epidermis
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
vibrissa
moderate moderate
regionalmoderate expression: see section 21 weak expression: see section 06 07 20
nasal cavity olfactory epithelium
weak weak
regionalweak expression: see section 08 09 10 11 12 13 14 15 16 17 18
nasal cavity respiratory epithelium
moderate moderate
regionalmoderate expression: see section 11 12 16 17 weak expression: see section 13
esophagus epithelium
weak weak
regionalweak expression: see section 08
pharynx epithelium
weak weak
regionalweak expression: see section 08 09 10
stomach
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08
rectum
weak weak
regionalweak expression: see section 11 12
midgut
weak weak
regionalweak expression: see section 09 10 11 12 13 14 15 16
urinary system
weak weak
regionalweak expression: see section 12
bladder
weak weak
regionalweak expression: see section 11 12
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30801
Entity Detected:Rab25, RAB25, member RAS oncogene family ( MGI:1858203)
Sequence:sense strand is shown

>T30801
CGCTGGCTAAAGGAGCTGTATGACCATGCCGAAGCCACGATTGTTGTCATGCTCGTGGGGAACAAAAGTG
ACCTCAGCCAGGCCCGAGAGGTCCCTACTGAGGAGGCCTGCATGTTTGCTGAAAACAATGGCCTGCTGTT
CCTGGAGACGTCAGCCCTCGACTCCACCAATGTTGAGCTAGCCTTCCAGACTGTCCTCAAAGAGATCTTT
GCAAAGGTGTCCAAGCAGAAGCAGAACAGCACCCGGACCAGTGCCATCACCCTGGGCAATGCCCAAGCTG
GACAGGATCCTGGCCCTGGGGAAAAGAGGGCCTGTTGCATCAGCCTCTGACTTCAGCTATGGCCAGCCCT
GTGTCCTGCCTTCAGCTTTCAGATATCTCCACCTCCCTTACTGCAGGACTTCTCCCTACCCTGGAGTCCC
TGATCACTAGCACGCAGGGTCATCATGCCCTACCATGTGATCTGCCCACCATATTCCTCTCCATCACCTG
GACCCCTTACAGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3968810), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 15555. Forward Primer - name:015555_F_IRAV16-19_I10_Rab25, sequence:CGCTGGCTAAAGGAGCTG; Reverse Primer - name:015555_R_SP6_IRAV16-19_I10_Rab25, sequence:GCCTGTAAGGGGTCCAGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15394 same embryo
 EMAGE:15399 same embryo
 EMAGE:15397 same embryo
 EMAGE:15398 same embryo
 EMAGE:15396 same embryo
 EurExpress:euxassay_010599 same experiment
 MGI:4827569 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS