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EMAGE:15500

Bspry B-box and SPRY domain containing ( MGI:2177191)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15500 EMAGE:15500 EMAGE:15500 EMAGE:15500 EMAGE:15500
"Pseudo-wholemount" of euxassay_010677. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010677_01 euxassay_010677_02 euxassay_010677_03 euxassay_010677_04
EMAGE:15500 EMAGE:15500 EMAGE:15500 EMAGE:15500 EMAGE:15500
euxassay_010677_05 euxassay_010677_06 euxassay_010677_07 euxassay_010677_08 euxassay_010677_09
EMAGE:15500 EMAGE:15500 EMAGE:15500 EMAGE:15500 EMAGE:15500
euxassay_010677_10 euxassay_010677_11 euxassay_010677_12 euxassay_010677_13 euxassay_010677_14
EMAGE:15500 EMAGE:15500 EMAGE:15500 EMAGE:15500 EMAGE:15500
euxassay_010677_15 euxassay_010677_16 euxassay_010677_17 euxassay_010677_18 euxassay_010677_19
EMAGE:15500 EMAGE:15500 EMAGE:15500 EMAGE:15500 EMAGE:15500
euxassay_010677_20 euxassay_010677_21 euxassay_010677_22 euxassay_010677_23 euxassay_010677_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15500Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15500_wholemount_strong.wlz
15500_wholemount_moderate.wlz
15500_wholemount_weak.wlz
15500_wholemount_possible.wlz
15500_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15500_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
thymus primordium
moderate moderate
regionalmoderate expression: see section 11 weak expression: see section 10 12 13 14
sublingual gland primordium
weak weak
regionalweak expression: see section 10 16
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 07 08 weak expression: see section 09 17 18 19
pituitary gland
weak weak
regionalweak expression: see section 10 11 12 13 14 15
cornea epithelium
weak weak
regionalweak expression: see section 01 02 03 04 05 23 24
naris
weak weak
regionalweak expression: see section 13 14 16 17
nasal cavity olfactory epithelium
weak weak
regionalweak expression: see section 09 10 11 12 13 15 16 17 18 19
vomeronasal organ
weak weak
regionalweak expression: see section 13 16
stomach
weak weak
regionalweak expression: see section 05 06 07 08 09 10
hindgut
weak weak
regionalweak expression: see section 15 16
midgut
weak weak
regionalweak expression: see section 10 11 12 13 14 15 16 17 18 19
bladder
weak weak
regionalExpression in the fundus region.
metanephros
weak weak
regionalweak expression: see section 08 09 10 11 17 18 19 20
male reproductive system
weak weak
regionalweak expression: see section 14 15
urethra of male
weak weak
regionalweak expression: see section 14 15
lung
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31207
Entity Detected:Bspry, B-box and SPRY domain containing ( MGI:2177191)
Sequence:sense strand is shown

>T31207
GCACCAACATGGTGGACATGATCACCCACCTGGATGACCTCCAGCTGATTCAGAGGGAACAGGAGATTTT
TGAGAGGGCCGAGGAAGCAGAGGGCATTTTGGATCCCCAGGATTCGGAGAAATTAAGCTTTAATGAGAAG
TGTGCTTGGAGCCCACTACTGACACAACTGTGGGCAGCATCGGTTCTCGGGTCTCTCTCAGGCGTGGAGG
AGGTGCTGATTGATGAAAGGACTGTCAGCCCCTTACTGCATCTGTCTGAAGATCGAAGGACCCTGACCTT
CATTGCCAAGAAGTCTAAGGTCTGCTCAGATGAACCAGAGCGCTTTGACCACTGGCCCAATGCCCTGGCT
GCCACTGCCTTCCAGACTGGGCTCCATGCCTGGATAGTGAACGTTAAACACAGTTGTGCTTATAAAGTGG
GTGTGGCCTCAGATCAGCTGCCCCGAAAGGGCTCTGGCAATGACTGCCGCCTGGGCCACAATGCCTTCTC
CTGGGTCTTCTCCCGCTATGATCAAGAGTTCTGTTTCTCACACAATGGGTATCATGAGCCTCTGCCACTA
CTTCGGTGCCCAGCACAGCTGGGCATGCTGCTGGACCTGCAGGCAGGAGAGCTGGTCTTCTACGAGCCTG
CCTCAGGGACGGTGCTTCATATCCACCGTGCGTCCTTCCCTCAGGTGCTCTTCCCAGTCTTTGCTGTGGC
TGACCAGCTCATCTCCATCGTTCGCGGACCTCTGGCCACAGTGAGACTAGATTCACCTCCCCATCACCCT
TGCAGGTCAGGCCATGCTTCCAGGTAGTGTTCTTTTCCTCAATGACATGAGTGTTCCCCAGGGCAACTTC
TGGATAGCAGCTTCAGACTCAGACATAACCTTTCAATGTTTCATCTGTGGGGATCCTGTTTCTTTTCTGT
TGGCCTTAGTCTCCCAAACTCACCATGCTTGTGCCCTTCCA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3492107), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 13927. Forward Primer - name:013927_F_IRAV04-07_E14_Bspry, sequence:GCACCAACATGGTGGACA; Reverse Primer - name:013927_R_SP6_IRAV04-07_E14_Bspry, sequence:TTGGAAGGGCACAAGCAT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15505 same embryo
 EMAGE:15502 same embryo
 EMAGE:15504 same embryo
 EMAGE:15501 same embryo
 EMAGE:15503 same embryo
 EurExpress:euxassay_010677 same experiment
 MGI:4823511 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS