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EMAGE:15512

Tbx3 T-box 3 ( MGI:98495)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15512 EMAGE:15512 EMAGE:15512 EMAGE:15512 EMAGE:15512
"Pseudo-wholemount" of euxassay_010709. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010709_01 euxassay_010709_02 euxassay_010709_03 euxassay_010709_04
EMAGE:15512 EMAGE:15512 EMAGE:15512 EMAGE:15512 EMAGE:15512
euxassay_010709_05 euxassay_010709_06 euxassay_010709_07 euxassay_010709_08 euxassay_010709_09
EMAGE:15512 EMAGE:15512 EMAGE:15512 EMAGE:15512 EMAGE:15512
euxassay_010709_10 euxassay_010709_11 euxassay_010709_12 euxassay_010709_13 euxassay_010709_14
EMAGE:15512 EMAGE:15512 EMAGE:15512 EMAGE:15512 EMAGE:15512
euxassay_010709_15 euxassay_010709_16 euxassay_010709_17 euxassay_010709_18 euxassay_010709_19
EMAGE:15512 EMAGE:15512 EMAGE:15512 EMAGE:15512
euxassay_010709_20 euxassay_010709_21 euxassay_010709_22 euxassay_010709_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15512Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15512_wholemount_strong.wlz
15512_wholemount_moderate.wlz
15512_wholemount_weak.wlz
15512_wholemount_possible.wlz
15512_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15512_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
hand skin
moderate moderate
regionalmoderate expression: see section 04 05 20 21
foot skin
moderate moderate
regionalmoderate expression: see section 06 07 08 19 20
adrenal medulla
moderate moderate
regionalmoderate expression: see section 07 08 09 16 17 18
vibrissa
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 20 21 22
hypothalamus
strong strong
regionalstrong expression: see section 11 12 13 moderate expression: see section 14
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 16 17 18 19 20 21
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 06 07 16
endolymphatic duct
moderate moderate
regionalmoderate expression: see section 01 02 03 21 22 23
cochlea
moderate moderate
regionalmoderate expression: see section 07 08 18 19
utricle
moderate moderate
regionalmoderate expression: see section 01 02 03 04 19 20 21 22 23
tongue epithelium
strong strong
regionalstrong expression: see section 09 10 11 12 moderate expression: see section 13 14
stomach
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08
midgut
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14 15 16 17
bladder
moderate moderate
regionalmoderate expression: see section 12 13
kidney calyx
moderate moderate
regionalmoderate expression: see section 07 08 09 10 16 17 18 19
genital tubercle of male
moderate moderate
regionalmoderate expression: see section 11 12 13 14
left lung
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 10 11
right lung
moderate moderate
regionalmoderate expression: see section 12 weak expression: see section 13 14 15 16 17 18 19 20 21 22
trachea
moderate moderate
regionalmoderate expression: see section 11 12
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38116
Entity Detected:Tbx3, T-box 3 ( MGI:98495)
Sequence:sense strand is shown

>T38116
TACTGAAACCGACTTCCAGGAGCGGCTTTTCCAACACACTCCACGCACCAGGACAGCCCCTGCAGCGGCT
ATGTCTCCAGATCTGCTGCCGCTTTGTCCTCTTTAAAAGCGGCGTTCTCTTCCCCCCAAAGACACTTTCC
CCCTCCCTTTCAAACGCTTTATTTGTAATTTGCCCTCTTTTTTATTTTAAACAAAACTATGCGTCGCTGT
TGAATTTTTTAATTATTATTTTTGAAAAGCAACAACAAAAGCGGAGCCAAGCCAGCAGCTGCGGACTGGT
TCCCTGTCTGGAGTGGATGAGCCTCTCCATGAGAGATCCGGTTATCCCTGGGACAAGCATGGCCTATCAT
CCCTTCCTACCTCACCGGGCGCCGGACTTCGCCATGAGCGCGGTACTGGGCCACCAGCCGCCTTTCTTCC
CCGCGCTAACGCTGCCTCCCAACGGCGCCGCGGCGCTCTCGCTCCCCGGAGCTCTGGCCAAGCCCATCAT
GGATCAGTTAGTGGGGGCTGCTGAGACCGGCATCCCTTTCTCATCCCTGGGACCCCAGGCACATCTGAGG
CCTCTGAAGACCATGGAACCCGAAGAAGACGTAGAAGATGACCCTAAGGTGCACCTGGAGGCCAAGGAAC
TTTGGGACCAGTTTCACAAGCGGGGTACAGAGATGGTCATCACGAAGTCAGGAAGGCGAATGTTCCCTCC
GTTTAAAGTGAGGTGCTCTGGACTGGATAAAAAGGCCAAGTATATTTTATTGATGGACATTATAGCTGCT
GACGACTGTCGATATAAATTTCACAACTCTCGGTGGATGGTGGCCGGTAAGGCAGACCCCGAAATGCCAA
AGAGAATGT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 104327. Forward Primer - name:104327_F_cDNA_Tbx3, sequence:TACTGAAACCGACTTCCAGGAG; Reverse Primer - name:104327_N_SP6_cDNA_Tbx3, sequence:ACATTCTCTTTGGCATTTCGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15517 same embryo
 EMAGE:15514 same embryo
 EMAGE:15513 same embryo
 EMAGE:15515 same embryo
 EMAGE:15516 same embryo
 EurExpress:euxassay_010709 same experiment
 MGI:4828625 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS