EMAGE mouse gene expression spatial database

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EMAGE:15810

Hpn hepsin ( MGI:1196620)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013178. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013178_01	euxassay_013178_02	euxassay_013178_03	euxassay_013178_04

euxassay_013178_05	euxassay_013178_06	euxassay_013178_07	euxassay_013178_08	euxassay_013178_09

euxassay_013178_10	euxassay_013178_11	euxassay_013178_12	euxassay_013178_13	euxassay_013178_14

euxassay_013178_15	euxassay_013178_16	euxassay_013178_17	euxassay_013178_18	euxassay_013178_19

euxassay_013178_20	euxassay_013178_21	euxassay_013178_22	euxassay_013178_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15810_wholemount_strong.wlz

15810_wholemount_moderate.wlz

15810_wholemount_weak.wlz

15810_wholemount_possible.wlz

15810_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15810_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 10 11 12 14

submandibular gland primordium

strong

regional

strong expression: see section 06 07 moderate expression: see section 08 09 16 17 18 19

pancreas

moderate

regional

moderate expression: see section 10 11 12 15 16

thyroid gland

weak

regional

weak expression: see section 09 10 11 15

anterior naris epithelium

weak

regional

weak expression: see section 12 15 16

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 11 12 15 16

stomach

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11

rectum

moderate

regional

moderate expression: see section 14

midgut

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18

liver

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

kidney calyx

moderate

regional

moderate expression: see section 08 09 10 11 12 18 19 20

kidney pelvis

moderate

regional

moderate expression: see section 10

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39055
Entity Detected:	Hpn, hepsin ( MGI:1196620)
Sequence:	sense strand is shown >T39055 CGAGTATTTGCTGGTGCTGTAGCCCGGACCTCACCCCATGCTGTGCAACTGGGGGTTCAGGCTGTGATCT ATCATGGGGGCTACCTTCCCTTTCGAGACCCTACTATTGACGAAAACAGCAATGACATTGCCTTGGTCCA CCTCTCTAGCTCCCTGCCTCTCACAGAATACATCCAGCCAGTGTGTCTCCCTGCTGCGGGACAGGCCCTG GTGGATGGCAAGGTCTGTACTGTGACCGGCTGGGGTAACACACAGTTCTATGGCCAACAGGCTATGGTGC TCCAAGAGGCCCGGGTTCCCATCATAAGCAACGAAGTTTGCAACAGCCCCGACTTCTACGGGAATCAGAT CAAGCCCAAGATGTTCTGTGCTGGCTATCCTGAGGGTGGCATTGATGCGTGCCAGGGCGACAGTGGAGGC CCCTTTGTGTGTGAAGACAGCATCTCTGGGACATCAAGGTGGCGGCTATGTGGCATTGTAAGCTGGGGTA CCGGCTGTGCTTTGGCCCGGAAGCCAGGAGTGTACACCAAAGTCACTGACTTCCGGGAGTGGATCTTCAA GGCCATAAAGACTCACTCCGAAGCCAGTGGCATGGTGACTCAGCCCTGATCCCGCCTCATCTCGCTGCTC CGTGCTGCACTAGCATCCAGAGTCAGAGTTGGTCTGGTGGCTCCAGCCCCACGTGGTAGGCTCCACACTG GGCCTCACATGGAATGGTTTCCTGCTCAGATCCAGTCCAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 97880. Forward Primer - name:097880_F_cDNA_Hpn, sequence:CGAGTATTTGCTGGTGCTGTAG; Reverse Primer - name:097880_N_SP6_cDNA_Hpn, sequence:GTGGACTGGATCTGAGCAGGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15813 same embryo
	EMAGE:15812 same embryo
	EMAGE:15809 same embryo
	EMAGE:15814 same embryo
	EMAGE:15811 same embryo
	EurExpress:euxassay_013178 same experiment
	MGI:4825445 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source