EMAGE mouse gene expression spatial database

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EMAGE:15905

Hs3st4 heparan sulfate (glucosamine) 3-O-sulfotransferase 4 ( MGI:1333792)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013327. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013327_01	euxassay_013327_02	euxassay_013327_03	euxassay_013327_04

euxassay_013327_05	euxassay_013327_06	euxassay_013327_07	euxassay_013327_08	euxassay_013327_09

euxassay_013327_10	euxassay_013327_11	euxassay_013327_12	euxassay_013327_13	euxassay_013327_14

euxassay_013327_15	euxassay_013327_16	euxassay_013327_17	euxassay_013327_18	euxassay_013327_19

euxassay_013327_20	euxassay_013327_21	euxassay_013327_22	euxassay_013327_23	euxassay_013327_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15905_wholemount_strong.wlz

15905_wholemount_moderate.wlz

15905_wholemount_weak.wlz

15905_wholemount_possible.wlz

15905_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15905_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 07 08 09 10 16 18 moderate expression: see section 17

thalamus mantle layer

strong

regional

strong expression: see section 08 09 10 16 moderate expression: see section 11 17

cerebral cortex mantle layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 15 16 18 19 20 22 23 24 moderate expression: see section 12 13 14 21

telencephalon mantle layer

strong

regional

strong expression: see section 02 03 04 05 15 16 22 23 24 moderate expression: see section 06 07 12 13 14 20 21

medulla oblongata alar plate mantle layer

moderate

regional

moderate expression: see section 09 10 11 12 13

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 07 08 moderate expression: see section 09 10 11 12 13

pons mantle layer

strong

regional

strong expression: see section 07 08 15 16 17 18 19 moderate expression: see section 05 06 09 10 11 12 13

midbrain mantle layer

strong

regional

strong expression: see section 05 06 07 08 16 17 18 moderate expression: see section 09 10 11 12 13 14 15

spinal cord mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39879
Entity Detected:	Hs3st4, heparan sulfate (glucosamine) 3-O-sulfotransferase 4 ( MGI:1333792)
Sequence:	sense strand is shown >T39879 GCAGATAACCATGGAGAAGACCCCCAGTTATTTTGTGACGAATGAGGCTCCCAAGCGCATCCACTCTATG GCCAAGGATATCAAACTCATTGTGGTGGTACGAAACCCAGTGACCAGGGCCATTTCTGACTATACCCAGA CACTGTCAAAAAAGCCAGAGATCCCCACCTTTGAGGTTCTGGCCTTCAAAAACCGGACCCTGGGGCTGAT TGATGCCTCTTGGAGCGCCATTCGCATTGGGATCTATGCTTTGCACCTGGAGAACTGGCTGCAGTACTTT CCCCTTTCTCAGATCCTGTTTGTCAGTGGTGAGCGACTCATAGTGGACCCTGCAGGGGAGATGGCCAAGG TCCAGGATTTCCTAGGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 149451. Forward Primer - name:149451_F_cDNA_LOC384670, sequence:GCAGATAACCATGGAGAAGACC; Reverse Primer - name:149451_N_SP6_cDNA_LOC384670, sequence:GCCTAGGAAATCCTGGACCTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15902 same embryo
	EMAGE:15901 same embryo
	EMAGE:15906 same embryo
	EMAGE:15903 same embryo
	EMAGE:15904 same embryo
	EurExpress:euxassay_013327 same experiment
	MGI:4825455 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source