EMAGE mouse gene expression spatial database

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EMAGE:15984

Iqsec3 IQ motif and Sec7 domain 3 ( MGI:2677208)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013432. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013432_01	euxassay_013432_02	euxassay_013432_03	euxassay_013432_04

euxassay_013432_05	euxassay_013432_06	euxassay_013432_07	euxassay_013432_08	euxassay_013432_09

euxassay_013432_10	euxassay_013432_11	euxassay_013432_12	euxassay_013432_13	euxassay_013432_14

euxassay_013432_15	euxassay_013432_16	euxassay_013432_17	euxassay_013432_18	euxassay_013432_19

euxassay_013432_20	euxassay_013432_21	euxassay_013432_22	euxassay_013432_23	euxassay_013432_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15984_wholemount_strong.wlz

15984_wholemount_moderate.wlz

15984_wholemount_weak.wlz

15984_wholemount_possible.wlz

15984_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15984_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 08 09 weak expression: see section 10 11 12 13 14 15 16 17 18 19

telencephalon mantle layer

moderate

regional

moderate expression: see section 07 08 09 weak expression: see section 03 04 05 06 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

hindbrain

moderate

regional

moderate expression: see section 07 08 09 weak expression: see section 04 05 06 10 11 12 13 14 15 16 17 18 19 20 21

midbrain

moderate

regional

moderate expression: see section 07 08 09 weak expression: see section 06 10 11 12 13 14 15 16 17 18 19 20

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 07 08 18 19

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 09 19 20 21 22 23

spinal cord

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17

cervico-thoracic ganglion

weak

regional

weak expression: see section 10 17

cervical ganglion

weak

regional

weak expression: see section 09 17

thoracic ganglion

weak

regional

weak expression: see section 14 15

dorsal root ganglion

weak

regional

weak expression: see section 07 09 10 11 12 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T45316
Entity Detected:	Iqsec3, IQ motif and Sec7 domain 3 ( MGI:2677208)
Sequence:	sense strand is shown >T45316 CTGGCCACTGGTCACTATTGTAGAAAGAGAATGCCTTGGCAAGATGGTTTGCTTGACCCAACCATCCTTC ACTAACTGATTTGAGTACCTTGCCACCAATGTGTAACCACCTTCTCACTCTTCTAGGGGGTCCCACATGG CCAGATGGACCTTCTCCTCTGTGTTCACCATGCTCCCCTTATTAGATTGTAGATTGAAGGAAACTGAAGC CAAGGGGCCTGTGGGCTGATGGGCTTGCAATCTTGGTCTATCAGTGTCCATGTCTTGGGCCAAGAACAAA AGGCTGAACCTGCCAGCAGCACCCTCCAGGACAGGGGCCGGCTACCCCAGCATAAGGTGTATCTATGCCC CCAGCTGAATCAAGGAAAGCTATCCCTGGCTCCCTGGCAGGACCTAGGCGTAGTAGAAGGGAGCTCTGTC TACTGCGTCTGTTATCCAAACCTATAGTCAGGAAACTGGTTCTTAGCTCCCCAGGGATGTTGGCTCCCTG AACCATGTCCACTCTCCTGCCTTCCCCAGACCCAGGAAAGACCCAAGAAAGAGAAGGGTCCAGGGTATTC AGTTCTGTCTGGGTCAGTTCAACGTGGCCCTGCGACGGTGAGGTCATGTCTGCAAAGTGCACTTTTAAAG AACATTTCTTGGTTCTCTAAGCCTTAAACGCTCGGCCAGTACCGAGTGTACACAGGCAGTTATCCGTAAA CACATG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 202402. Forward Primer - name:202402_F_exon_AK122446, sequence:CTGGCCACTGGTCACTATTGTA; Reverse Primer - name:202402_N_SP6_exon_AK122446, sequence:CATGTGTTTACGGATAACTGCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15988 same embryo
	EMAGE:15987 same embryo
	EMAGE:15989 same embryo
	EMAGE:15985 same embryo
	EMAGE:15986 same embryo
	EurExpress:euxassay_013432 same experiment
	MGI:4825616 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source