EMAGE mouse gene expression spatial database

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EMAGE:17127

Mmgt1 membrane magnesium transporter 1 ( MGI:2384305)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001526. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001526_01	euxassay_001526_02	euxassay_001526_03	euxassay_001526_04

euxassay_001526_05	euxassay_001526_06	euxassay_001526_07	euxassay_001526_08	euxassay_001526_09

euxassay_001526_10	euxassay_001526_11	euxassay_001526_12	euxassay_001526_13	euxassay_001526_14

euxassay_001526_15	euxassay_001526_16	euxassay_001526_17	euxassay_001526_18	euxassay_001526_19

euxassay_001526_20	euxassay_001526_21	euxassay_001526_22	euxassay_001526_23	euxassay_001526_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17127_wholemount_strong.wlz

17127_wholemount_moderate.wlz

17127_wholemount_weak.wlz

17127_wholemount_possible.wlz

17127_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17127_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

moderate

regional

moderate expression: see section 04 05

axial muscle

moderate

regional

moderate expression: see section 20 21

thymus primordium

weak

regional

weak expression: see section 11 12 13 14

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 17 18 19 20

brain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vibrissa

moderate

regional

moderate expression: see section 07 08 09 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 07 17

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 07 17 18

spinal cord

moderate

homogeneous

moderate expression: see section 06 07 08 09 10 11 12 13 14

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 09 15 16

cervical ganglion

moderate

regional

moderate expression: see section 08 09 16

thoracic ganglion

moderate

regional

moderate expression: see section 09 10 11 12

neural retina

moderate

regional

moderate expression: see section 01 02 03 04 05 23 24

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 13 15 16 17 18 19

lower jaw incisor

moderate

regional

moderate expression: see section 12 13 16 17

upper jaw incisor

moderate

regional

moderate expression: see section 12 13 16 17

renal cortex

moderate

regional

moderate expression: see section 05 06 07 08 14 15 16 17 18

lung

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 11 12 14 15 16 17 18 19 20 21 22 23

tail dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4515
Entity Detected:	Mmgt1, membrane magnesium transporter 1 ( MGI:2384305)
Sequence:	sense strand is shown >T4515 CCACGCGTCCGCCGCCGGTGAAAGGAGGAGTACCTCTGCCGCCCTTCGTCTCCTTTTGTTGGGCTGCCGC TCCTTCTGAATCATGGCGCCGTCGCTGTGGAAGGGGCTTGTAGGTGTCGGGCTTTTTGCCCTAGCCCACG CTGCCTTTTCAGCTGCGCAGCATCGTTCTTATATGCGACTAACAGAAAAGGAAGATGAATCATTACCAAT AGATATAGTTCTTCAGACACTTCTGGCCTTTGCAGTTACCTGTTATGGCATAGTTCATATCGCAGGGGAG TTCAAAGACATGGATGCCACTTCAGAATTAAAGAATAAGACATTTGATACCTTAAGGAATCACCCATCTT TTTATGTGTTTAACCATCGTGGTCGAGTGCTGTTCCGGCCTTCAGATGCAACAAATTCTTCAAACCTAGA TGCATTGTCCTCTAATACATCGTTGAAGTTACGAAAGTTTGACTCACTGCGCCGTTAAGCTTTTTACAAA TTAAATAACAGGACAGACACAGAATTGAGTATTGGAGTTTGGGGTGTAAAACACTCCCCCCAAGATCAGT ATTTATATTGCTATTTCAGAGCTAGTTAAACTAATCTATGGGCCCTTGTTTATATATTATCGATCCGATC AAATGGAGTATGAATTATCTGTGAAATGAGTCTTTGAACTTTCGTATAGAGTTCTTTTGTTGATTTGAAA CAATTCCCATGTTTTGTAAAAGTCACTGTTTCAAACATAATTCCCTGAAAAAATGTTCGGATTTTTGTGA TTATTTATTTTCATAGATTTCTTTTCTTTTGCACTACAGTTTTTAGGAATCCTGGAAATACCATATACCA TCTGTGTTTTGCAGCACAAGAATCAAATGGTCTTCAGTTCTTATCAGACCAACTTCTTTAAGAAGACCAA AATGGTGACTTTACAGTTTTTCTTTGGTCCCCTAAAAAGTGACTCTACTTTTACAGATACTTGCCAATTT TTACTTTATTAATGGTTTCTCTCCGGACTCCACTCCCTGATACTGTCTAACATAAGCTGTCTTGTTTCAT CATTTCTAGGATTCTGTGTGCAGTGAGCAATTTTTGTGTCAGAAATTCTATCATAACATATATATTTAAC AAGCTCATCTTGCTGTAAAACTACTCATGTTCTTTTGTTGTAAAATTTTCACTGATTGTATAATGTAAGA CTAGTTGAAAGTAGACCAAAAAGACAACTTGTGACTTCATTATCTTGCCTGAGTAGAATAGTATTGATTG AGGAAAACTGATCGTCATTAGCCAGTCTCACTCATTATAAGTCTGTTGGTTTATGTTGTACTAAACTAGT GAATTAGTGAAAGTAAAGGAAATGGCTCATTTTATCTAAAGGCACATGTGCAAAGAAACTGGGCCAAATA TGTTCTAAATATTTGGAGTTCATCTATTTGAATTCTCTTAGAAAATTCAGGTCATTTAGGAAGTTGGTTC TCAGCTTCTGACTGAGAGCTGGCCTGGAGCTTCCCATCTTCACTCTGCGGTGCTGAGGGGGAGGATGACA TGCCATTGGATGCTTTTCAGAAACAGTAGTTTACTCTGACAACATTGGAGTAGCTTTTTCAGCTAGAAAG CTAAGAAGCTCAACATTACTGTAGAGATTATTGCTTCCCACGAGCATGAACACATTACTTGTGTTAAACT CTATAGGTGTAAAGGGAATCACTGCGGATTTTGATAGGAACCCCGCCTGATGCTTTTTGGTTTGTTTCAG GCAGGGTCTTCATATCGAGAAGCTCTTTTACTTGGTAGCATTTTTTGATTAGGAATGAACCTCAGCCTTT CTGCCCAGTCATACAGAGCCTCAAAGGCCAAGGTTATTTTGTCATTTCTTTGTTGGTTTTGGTTTTTGTT TGTAAACAGGATTCCGTATAACCTGAGCTAGCCTCAAATGTACTATGTAGCCGAGGATGATCCTCATGCC TCCACCGTGTCAGTGCTAAGATTATAGGTGCTGTCACTACACCTGGTTTTTTGAGATCCTGGGCACTGAA CCCAGGGCCTCATGCATGCTAGGCAAGCACTATACCAGCTGAACTACATCCCAGGCCCTTGACATTGAAT TATGATTCTTGAGCTCTGTTTTGCTAACTAAGTCTATGGAGCATTGACTGTTCTCTAATGAGAACAAACT TGGAAATCTGCTGATTACCTACAATAATTTAGACATAGGCTAAAAGTGTGAAGTCAAGCCAGGTGTGGTG GCATGTGCTTGTAATCCTAGCACTTACAAGGCTGAAACAGGATCACAAGTTTAGGGCAGAACTGGGCTAT CTTAATGAACCCCTGTCTTAACCCCAACCCCCAAAAAGCGAACCCAAGGACTAATGCGATAGCTAAATCA AAATGGCGTGCAGGACGCATGCTGTACATCAGGCTTTTCTGCCATACTCCTCGACTATTACTTGACTGAA GAGCCTCTTGTTTTCTTCTCCTTGGAGACTGTTTTCCCTTCTTAATGTGTTTTCATAACAATGTTACTGA GAATCTGCGTGTTTTGTACTTCCATTCTTTAAAGGATGTATAATTGCCAGATGTGGCTCATGCCTATAAT ACTAACACTCAGGAGGCTAAGGCAGGAAAGTTTTCACACAGGTTCAAGACCAGAATTAGATACACAGTGA GACCTTATCTTTAAAGAAGTTATACAAATTTTAAAGGGTACAATTAAGTAACTTTAAATATACTCACAAA ATTATGCAACCACTTGATAAATCTAGGCCATTTCACTCCTCTCCAAGAAGCCCTGTAACACCAATAAACC CATTGTTGAATATATTTTCTCTCTTTAAATGACCTTGCCTTGTCCAATAAATAAGCGATTGTCAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4954726 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4954726 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17131 same embryo
	EMAGE:17130 same embryo
	EMAGE:17129 same embryo
	EMAGE:17128 same embryo
	EurExpress:euxassay_001526 same experiment
	MGI:4826402 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source