EMAGE mouse gene expression spatial database

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EMAGE:17130

Atp1a2 ATPase, Na+/K+ transporting, alpha 2 polypeptide ( MGI:88106)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001505. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001505_01	euxassay_001505_02	euxassay_001505_03	euxassay_001505_04

euxassay_001505_05	euxassay_001505_06	euxassay_001505_07	euxassay_001505_08	euxassay_001505_09

euxassay_001505_10	euxassay_001505_11	euxassay_001505_12	euxassay_001505_13	euxassay_001505_14

euxassay_001505_15	euxassay_001505_16	euxassay_001505_17	euxassay_001505_18	euxassay_001505_19

euxassay_001505_20	euxassay_001505_21	euxassay_001505_22	euxassay_001505_23	euxassay_001505_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17130_wholemount_strong.wlz

17130_wholemount_moderate.wlz

17130_wholemount_weak.wlz

17130_wholemount_possible.wlz

17130_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17130_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forelimb

moderate

regional

moderate expression: see section 01 02 03 04 05 06 22 23 24

hindlimb

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 17 18 19 20 21 22 23 24

vertebral axis musculature

moderate

regional

moderate expression: see section 02 04 05 06 07 08 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

brain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 06 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 08 09 17

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 17 18 19 20

spinal cord

moderate

homogeneous

moderate expression: see section 07 08 09 10 11 12 13 14 15

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 09 16

cervical ganglion

moderate

regional

moderate expression: see section 09 16

thoracic ganglion

moderate

regional

moderate expression: see section 11 12

cochlea

strong

regional

strong expression: see section 01 02 03 22 23 24

not examined

regional

not examined expression: see section 02 03 04 05 24

neural retina

strong

regional

strong expression: see section 02 03 04 05 24

naris

moderate

regional

moderate expression: see section 13 14 16 17 18 19

viscerocranium

moderate

regional

Expression in the turbinate bone.

tongue

weak

regional

weak expression: see section 12 13 14 15 16 17 18

tail mesenchyme

moderate

regional

moderate expression: see section 11 12 13 14 15 16

tail dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 13 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4513
Entity Detected:	Atp1a2, ATPase, Na+/K+ transporting, alpha 2 polypeptide ( MGI:88106)
Sequence:	sense strand is shown >T4513 CCCACGCGTCCGAGCTGCCCCAGACAGGCGGTGTGGTCTTGGGATCCTCCTGGTGACCTTTTCCAGCCTA GGTCCCCTCAGCCACTCTGCCCCAAGATGGGTCGTGGGGCAGGGCGTGAGTACTCGCCTGCTGCCACCAC TGCGGAAAATGGGGGTGGCAAGAAGAAACAGAAAGAGAAGGAGCTCGATGAGCTGAAGAAGGAGGTTGCC ATGGATGACCACAAGCTGTCCTTGGATGAGCTGGGCCGAAAATACCAAGTGGATCTGTCTAAGGGCCTCA CCAATCAGCGAGCTCAGGACATTCTGGCTAGAGATGGACCCAACGCCCTCACTCCGCCCCCCACAACTCC TGAGTGGGTCAAGTTCTGTCGTCAGCTTTTTGGGGGCTTCTCTATCCTGCTGTGGATTGGGGCACTCCTC TGCTTCTTAGCCTACGGTATCCTGGCCGCCATGGAAGACGAACCATCCAATGATAATTTATATCTAGGTA TCGTGCTAGCGGCTGTAGTTATCGTCACCGGCTGCTTCTCCTACTACCAGGAAGCCAAGAGCTCCAAGAT CATGGACTCCTTCAAGAACATGGTGCCTCAGCAAGCTCTGGTCATCCGAGAGGGAGAAAAGATGCAGATC AATGCAGAGGAGGTGGTGGTGGGAGACCTGGTGGAGGTGAAGGGTGGAGACCGTGTCCCTGCTGACCTCC GGATCATCTCTTCCCACGGTTGCAAGGTGGATAACTCATCCCTAACAGGGGAGTCGGAGCCCCAGACCCG TTCTCCTGAGTTCACCCACGAGAACCCCTTGGAGACCCGCAATATCTGTTTCTTCTCTACCAACTGCGTG GAAGGCACTGCCAGGGGCATTGTGATTGCCACAGGTGACCGGACAGTGATGGGCCGCATAGCCACTCTTG CCTCTGGCCTAGAGGTGGGACAGACACCCATAGCCATGGAGATCGAGCATTTCATCCAGCTGATCACAGG GGTGGCTGTGTTCCTGGGGGTCTCCTTCTTCGTTCTGTCCCTCATCCTGGGCTACAGCTGGCTGGAGGCA GTCATCTTCCTCATCGGCATCATCGTAGCCAATGTGCCCGAAGGGTTGTTGGCCACCGTGACTGTGTGCC TGACGCTGACAGCCAAGCGCATGGCTCGCAAAAACTGCCTGGTGAAGAACCTGGAGGCCGTGGAGACGCT GGGCTCCACGTCCACCATCTGCTCAGACAAGACGGGCACCCTCACCCAGAACCGCATGACAGTGGCTCAC ATGTGGTTTGACAACCAGATCCATGAGGCTGACACCACAGAGGACCAGTCTGGGGCCACTTTTGACAAAC GGTCCCCCACATGGACAGCCCTGTCTCGGATCGCTGGTCTCTGCAATCGTGCTGTCTTCAAGGCTGGACA AGAGAACATCTCCGTGTCTAAGCGGGACACAGCTGGAGATGCTTCTGAGTCAGCTCTGCTCAAGTGCATT GAGCTGTCCTGTGGCTCAGTGAGGAAGATGAGGGACAGGAACCCTAAGGTGGCAGAAATCCCCTTCAACT CCACCAACAAATATCAGCTCTCCATCCATGAGAGGGAAGACAGTCCCCAGAGCCATGTGCTGGTGATGAA AGGTGCCCCAGAGCGCATCCTGGACCGATGCTCCACCATCCTGGTACAGGGCAAGGAGATCCCTCTTGAC AAGGAGATGCAAGATGCTTTTCAAAACGCCTACATGGAGCTGGGAGGACTTGGGGAACGAGTGCTGGGCT TCTGTCAGCTGAACCTGCCTTCTGGAAAGTTTCCTCGGGGCTTCAAATTTGATACAGATGAGCTGAACTT TCCCACAGAGAAGCTCTGTTTTGTGGGGCTCATGTCTATGATTGATCCCCCCAGAGCAGCCGTGCCGGAT GCCGTGGGCAAGTGCCGAAGCGCGGGCATCAAGGTGATCATGGTGACCGGGGATCACCCTATCACAGCTA AGGCCATTGCCAAAGGTGTGGGCATTATATCAGAGGGTAACGAGACTGTGGAAGACATTGCAGCCAGGCT CAACATTCCTGTGAGTCAAGTCAATCCCAGAGAAGCCAAGGCGTGTGTAGTGCACGGCTCAGACCTGAAG GACATGACGTCAGAGCAGCTGGATGAGATCCTCAGGGACCACACGGAGATTGTCTTTGCCCGGACCTCCC CTCAGCAGAAGCTTATCATTGTGGAAGGGTGTCAGAGGCAGGGAGCCATTGTGGCAGTGACTGGTGATGG GGTGAACGACTCCCCCGCGCTGAAGAAGGCTGACATTGGCATTGCCATGGGCATCTCCGGCTCCGATGTC TCTAAGCAGGCGGCTGACATGATCCTTCTCGACGACAACTTTGCCTCCATTGTGACAGGCGTGGAGGAGG GCCGCCTGATCTTTGACAACTTGAAGAAGTCCATCGCATACACCCTGACCAGCAACATCCCTGAGATCAC CCCCTTCCTGCTGTTCATCATTGCCAACATCCCCCTTCCACTGGGCACTGTGACCATCCTCTGCATCGAC CTGGGAACAGATATGGTTCCTGCTATCTCATTAGCATACGAAGCAGCTGAGAGTGACATCATGAAGAGGC AGCCACGAAACTCCCAGACAGACAAGCTGGTGAACGAGAGGCTTATCAGCATGGCTTACGGACAGATTGG CATGATCCAGGCTCTGGGTGGCTTCTTCACCTACTTTGTGATACTGGCAGAGAATGGGTTTCTACCATCG CGGCTGCTAGGGATCCGCCTGGACTGGGATGATCGGACTACCAACGACTTGGAGGACAGCTATGGACAAG AGTGGACCTACGAGCAGCGGAAGGTGGTGGAGTTCACATGCCACACGGCCTTCTTTGCCAGCATCGTGGT TGTGCAGTGGGCTGACCTCATCATCTGCAAGACCCGTCGCAACTCAGTGTTCCAGCAGGGCATGAAGAAC AAGATCCTGATTTTTGGGCTGCTAGAAGAGACGGCTTTGGCTGCCTTTCTGTCTTACTGCCCGGGCATGG GGGTTGCCCTCCGGATGTACCCACTCAAGGTCACGTGGTGGTTCTGTGCCTTCCCCTACAGTCTCCTCAT CTTCATCTATGATGAAGTTCGGAAGCTCATCCTACGGCGGTACCCTGGGGGCTGGGTGGAGAAGGAGACG TACTACTGAGCTCAGCGGCAAAGGAAGAACGAGGGGCAGGGGGAGATGGGGTGCTCCGGAGAGGTTGCTG GGGATTGTGGTGAAGGGAGGGACTGGGGAGTCAGAAGAAGGCACCTGAGGGAACTAAGATGGCAGCCTTG GGCAAATAAACTTGAGGAGACTGCTCCAGCTGCTCCACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:4955003 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4955003 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17131 same embryo
	EMAGE:17129 same embryo
	EMAGE:17128 same embryo
	EMAGE:17127 same embryo
	EurExpress:euxassay_001505 same experiment
	MGI:4823305 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source