EMAGE mouse gene expression spatial database

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EMAGE:17131

Noc3l nucleolar complex associated 3 homolog (S. cerevisiae) ( MGI:1932610)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001504. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001504_01	euxassay_001504_02	euxassay_001504_03	euxassay_001504_04

euxassay_001504_05	euxassay_001504_06	euxassay_001504_07	euxassay_001504_08	euxassay_001504_09

euxassay_001504_10	euxassay_001504_11	euxassay_001504_12	euxassay_001504_13	euxassay_001504_14

euxassay_001504_15	euxassay_001504_16	euxassay_001504_17	euxassay_001504_18	euxassay_001504_19

euxassay_001504_20	euxassay_001504_21	euxassay_001504_22	euxassay_001504_23	euxassay_001504_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17131_wholemount_strong.wlz

17131_wholemount_moderate.wlz

17131_wholemount_weak.wlz

17131_wholemount_possible.wlz

17131_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17131_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata alar plate mantle layer

moderate

regional

moderate expression: see section 11 12

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 08 09 15 16 not examined expression: see section 07

pons mantle layer

moderate

regional

moderate expression: see section 07 17

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 07 19 20 21 22

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 07 18

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

vagus x ganglion

strong

regional

strong expression: see section 07 08 17

ventral grey horn

moderate

regional

moderate expression: see section 09 10 11 12 13 14

cervico-thoracic ganglion

strong

regional

strong expression: see section 09 16

cervical ganglion

strong

regional

strong expression: see section 09 16

thoracic ganglion

strong

regional

strong expression: see section 11 12

dorsal root ganglion

strong

regional

strong expression: see section 06 07 08 13 14 15 16 17

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 11 12 13 16 17 18

urethra of male

moderate

regional

moderate expression: see section 14

nucleus pulposus

weak

regional

weak expression: see section 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4514
Entity Detected:	Noc3l, nucleolar complex associated 3 homolog (S. cerevisiae) ( MGI:1932610)
Sequence:	sense strand is shown >T4514 GAAAGGTTGCGCTCAGTGCACGTGTGTCTTTTCCACCCGGCTCCTGCGTGTTCCTCTGTCCGAGTGATCT TGTCTGTGGTACTCCTCGCAGGCCTAAGGATGAAGGCGAGAAGAAATAAAAAGCAGGTCCCAAGCTTTCG CAAGTTGATAAAAACTAGTAAAGTGAAACTTGAAAACAAATTGAAAAATAAGCAATTTAAACAACAAAGT ACCATCAAGAAGTACCGAAAAGAGCAGAGGAAACTAAGGCAAGCTGTAAAAGATGCTGTGTCTAAAAAAC CCATTCCGCTGGAGGACCCAAAGAGCAAACGGCCAGTTAAAAGGATGGAGAGGGAGGAGGACGAGGAGGA AGAAGCCCTGCCTTTGGATATGATGGACGAGGATGACCTACAGCTGATGAAGGATTTAGGACAAAAGGCA TCTTTTCTCACAAGAGATCTCTCTTCTAGTGAACCAGTCCATATCAAGAAACGAAAGCATGAGAGTGTGA TAGAGAAATATGAAAAAGTGCCAAGAACTCTGCAAACCGCACCGGAGAAGGAGTTGATTCACCTGCTCCC TATCAAGGACAAGAGTGGCATCATCCCTCAGGCTCGGGAGAAGCCGGTCACTGACTTGCAGCAAGAAGAG GAGGCTGAGGAAGAGCTGGAAGATGAGGAAGAGGTCATTGAAGACCCCAGGAAGGAGCTGACCATAGAAG AGCATGTGATCGAGAGAAAGAAGAAACTGCAGGACAAGAAAATACAGATTGCAGCCTTAGCATCTGCTAT TCTGTCAGATCCAGAAAGTCATATTAAAAAACTGAAAGAGTTGCGTTCCATGCTGATGGAGCAGGACCCT GATGTGGCTGTCACTGTCCGGAAGTTGGTTATCATCTCACTGATGGAGCTGTTTAAGGACATCACTCCCT CGTATAAAATCCGGCCTCTAACCGAAGCAGAAAAATCTACCAAGATTCGTAAAGAAACCCAGAAGTTAAG AGAATTTGAAGAAGGCCTGGTTAGCCAATACAAATTTTATTTGGAAAATCTGGAGCAAATAGTTAAAGAC TGGAAGCAAAGGAAGCTGAAGAAGAGCAACGTGGTGTCCTTAAAGGCCTACAAAGGGCTGGCTGAAGTGG CAGTGAAGAGCCTGTGTGAGCTGTTGGTGGCCCTGCCCCATTTTAACTTTCACAACAACATCATTGTACT GATTGTCCCTCTGATGAACGATGGGTCAAAACCGGTATCTGAAATGTGTTGCGAAGCAGTAAAGAAACTC TTTAAACAAGATAAATTAGGCCAAGCTTCTCTGGGTGTCATTAAAGTCATCTCTGGTTTTGTAAAGGGCA GAAATTACGAAGTTAGGCCAGAGATGTTAAAAACATTTTTATGCCTGCGGATCAAGGAGGTAGAAGTGAA AAAAGATACAGAGGACATTAACAAGCCCAAAAGGTTCATGACTTTCAAGGAGAAGAGAAAAACTCTATCA AGAATGCAAAGAAAGTGGAAGAAAGCAGAAGAGAAACTAGAGCGGGAACTTCGGGAGGCCGAAGCTTCGG AAAGCACTGAGAAAAAGCTGAAACTGCACACCGAGACTCTGAATATTGTGTTTGTGACCTACTTCAGAAT ACTGAAGAAGGCCCAGAAGTCACCTCTTCTGCCAGCGGTTCTAGAAGGCCTTGCCAAGTTTGCACATCTT ATAAATGTGGAGTTTTTTGATGATTTATTAGTGGTTCTTCATACCCTCATTGAGTCTGGGGAGCTAAGTT ATCAAGAAAGTCTCCACTGTGTTCAAACTGCTTTTCATATTCTTTCTGGACAAGGTGATGTTTTAAATAT TGACCCGATGAAATTCTATACACATCTCTACAAGACACTGTTCACATTACATGCAGGTGCCACCAACGAC GGCATTGAGATTGTGCTCCACTGCCTCGATGTCATGCTAAGCAAGCGCAGAAAGCAGGTTTCTCATCAGC GAGCTCTTGCCTTCATCAAACGCCTTTGTACACTTGCTCTGCAGGTTCTTCCAAATTCAAGCATTGGCCT TTTAGCAACTACCAGGATATTGATGCATACTTTTCCCAGAACAGATCTCTTGCTTGATAACGAGTCTCAG GGCAGCGGGGTGTTTCTACCTGAGCTGGAGGAGCCAGAGTACTGTAATGCACAGAACACCGCACTGTGGG AGCTGCACACGCTTCGGAGACATTACCATCCCATTGTGCGAAGGTTTGCAGCACATCTGCTTGCTGGGGC ACCATCCGAAGGCTCTGAGGCCCTCAAGCCCGAGCTGAGCCGAAGATCTGCAGTTGAACTTTTTGAGACT TACAGCATGGCAGCAATGACATTCAATCCGCCTGTTGAATCTTCACACTCCAAAAGGAAGGATAAATTTT TACCAGGAGACTCATTTTTGAATGAAGATTTAAATCAACTAATCAAAAGATACTGTAATGAAGCTGCTCC TGAGACACCGCTGGATTTCGCCAAGTGTTTGGAAAGCTCATCCCGGCAGTACAGAGTGAATGGACTGTCT TAGATATCTGCATGTGCATGGCAGAGACACAAGGATATGTGAGGGCCTGTTTCTGTTTGTACAAGGAAGA CTTCCTAAGAATGATTGAGAACTGGAGCAGGGCCACAGAAGACCCTGCTCATTGGCTTGCTTTTCATGCC TTGCTCGGCCTGCCTTTTTATACAGTCCACACCAACTTGTCCAGGAACGGCACTGCCCATCAGTCATGAA ATCTACAAATGCCCTGCAGACATGCCATAGTCTAGTCTGTGGAGGCAGTCCTTCAGTTGAGATTTCCTCT GCTCAGGTTACTCCAGTTAAGTAGACAAAACCTAAACAAGACAGCTATTAAATTCTCTTTATAACTAAAA AAATATTGTTATTGCAGCACCATAGTTGAAAGCAAATAAAATATTTTGTGGGTTTTTTTTTAAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4954951 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4954951 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17130 same embryo
	EMAGE:17129 same embryo
	EMAGE:17128 same embryo
	EMAGE:17127 same embryo
	EurExpress:euxassay_001504 same experiment
	MGI:4826740 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source