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EMAGE:17132

Adhfe1 alcohol dehydrogenase, iron containing, 1 ( MGI:1923437)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17132 EMAGE:17132 EMAGE:17132 EMAGE:17132 EMAGE:17132
"Pseudo-wholemount" of euxassay_001519. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_001519_01 euxassay_001519_02 euxassay_001519_03 euxassay_001519_04
EMAGE:17132 EMAGE:17132 EMAGE:17132 EMAGE:17132 EMAGE:17132
euxassay_001519_05 euxassay_001519_06 euxassay_001519_07 euxassay_001519_08 euxassay_001519_09
EMAGE:17132 EMAGE:17132 EMAGE:17132 EMAGE:17132 EMAGE:17132
euxassay_001519_10 euxassay_001519_11 euxassay_001519_12 euxassay_001519_13 euxassay_001519_14
EMAGE:17132 EMAGE:17132 EMAGE:17132 EMAGE:17132 EMAGE:17132
euxassay_001519_15 euxassay_001519_16 euxassay_001519_17 euxassay_001519_18 euxassay_001519_19
EMAGE:17132 EMAGE:17132
euxassay_001519_20 euxassay_001519_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17132Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17132_wholemount_strong.wlz
17132_wholemount_moderate.wlz
17132_wholemount_weak.wlz
17132_wholemount_possible.wlz
17132_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17132_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
rib
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 19 20 21
anterior abdominal wall muscle
weak weak
regionalweak expression: see section 01 02 03 04 18 19 20 21
hypothalamus ventricular layer
moderate moderate
regionalmoderate expression: see section 12 13
diencephalon lateral wall ventricular layer
moderate moderate
regionalmoderate expression: see section 11 12
diencephalon meninges
moderate moderate
regionalmoderate expression: see section 13
olfactory cortex ventricular layer
moderate moderate
regionalmoderate expression: see section 09 10 14 15
telencephalon ventricular layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 14 15 16 17 18 19 20 weak expression: see section 04 05 21
medulla oblongata alar plate ventricular layer
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14
medulla oblongata basal plate ventricular layer
moderate moderate
regionalmoderate expression: see section 11 12 13
pons ventricular layer
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 weak expression: see section 05 06
midbrain ventricular layer
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14 15 16
ventral grey horn
moderate moderate
regionalmoderate expression: see section 11 12 13 weak expression: see section 10
spinal cord ventricular layer
moderate moderate
regionalmoderate expression: see section 11 12 13
otic capsule
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 15 16 17 18 19 20 21
viscerocranium
moderate moderate
regionalExpression in the turbinate bone.
kidney calyx
moderate moderate
regionalmoderate expression: see section 06 16 17 weak expression: see section 07 08 15
nucleus pulposus
weak weak
regionalweak expression: see section 08 09 10 11 12 13 14 15 16 17 18
basisphenoid bone
moderate moderate
regionalmoderate expression: see section 13 weak expression: see section 01 11 12
exoccipital bone
weak weak
regionalweak expression: see section 01 03 04 05 18 19 20
supraoccipital cartilage condensation
weak weak
regionalweak expression: see section 04 05
orbito-sphenoid
weak weak
regionalweak expression: see section 01 06 07 10 11 12 16 17 18 19
pectoral girdle and thoracic body wall skeleton
weak weak
regionalweak expression: see section 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4526
Entity Detected:Adhfe1, alcohol dehydrogenase, iron containing, 1 ( MGI:1923437)
Sequence:sense strand is shown

>T4526
CTGCCATGGCTGCAGCTGCACGCGCTCGAGTCACACACTTGCTGAGGCACCTACAAAGCACAGCATGCCA
GTGCCCAACTCATTCTCATACTTACTCCCAAGCCCCTGGACCTTCGGGAAAAACAGCAGATTACGCCTTT
GAGATGGCTGTCTCAAATATTAGATATGGAGCAGGGGTTACGAAGGAAGTAGGCATGGATCTACAGAATA
TGGGAGCTAAGAATGTTTGCTTGATGACAGACAAGAACCTCTCCCAGCTCCCTCCTGTACAAATAGTTAT
GGATTCCCTATCGAAGAATGGCATCAGTTTTCAGGTTTATGATGATGTAAGGGTGGAACCTACAGATGGA
AGCTTCATGGATGCAATTGAATTTGCCAAAAAGGGAGCGTTTGATGCCTATGTTGCTGTGGGTGGGGGCT
CCACCATGGACACCTGTAAAGCCGCCAATCTGTATGCATCCAGCCCTCACTCTGAGTTCCTTGATTATGT
CAATGCCCCCATTGGGAAGGGGAAGCCAGTAACTGTGCCTCTTAAACCTCTGATTGCAGTTCCAACTACC
TCAGGGACTGGCAGTGAGACTACCGGGGTTGCCATTTTTGACTATGAGCATTTGAAAGTAAAAACTGGCA
TTGCTTCACGAGCCATCAAACCCACCCTGGGACTGGTTGATCCCCTGCACACTCTCCACATGCCTTGCCA
GGTGGTTGCCAACAGTGGCTTCGATGTCCTTTGCCATGCTCTGGAGTCATACACCGCCATTCCCTACAGC
ATGCGGAGCCCCTGCCCTTCCAACCCCATCCAACGACCAGCATACCAGGGTAGCAACCCAATCAGCGACA
TCTGGGCAGTCCATGCACTGCAGATTGTTGCCAAATATCTGAAGAGGGCTGTCAGAAACCCAGATGATCT
GGAAGCAAGGTCTAAAATGCACTTGGCAAGTGCTTTTGCTGGCATTGGCTTCGGAAATGCTGGTGTTCAT
CTGTGCCATGGCATGTCTTACCCAATTTCAGGTTTAGTGAAGACATATAAAGCCAAGGAATATAATGTGG
ATCACCCTCTTGTGCCCCATGGCCTTTCTGTGGTGCTCACCTCTCCTGCAGTGTTCACCTTCACAGCCCA
GATGTTTCCAGAGCGGCACCTGGAGACGGCAGGAATATTAGGAGCCAACATCCGCACTGCCAGGATCCAA
GATGCTGGGCTTGTGTTGGCAGATGCCCTCCGAAAATTCCTATTTGACCTAAATGTTGATGACGGTCTGG
CTGCTCTTGGTTATTCCAAGGATGACATTCCTTCATTGGTGAAAGGAACACTGCCCCAGGAAAGGGTCAC
GAAGCTCGCACCTCGTGCCCAGTCAGAAGAGGATTTGTCTGCTTTGTTTGAAGCATCCATGAAACTGTAC
TAATTAACATATTAGTGAAAAGAATCCCCATTGGCCTTTGCGACAGAAGTTACAAATAGCAGAAGTTGGC
CAGAGCTGATGTTGCCGTCTTATCTCCACACTTAACTCACCTATTGCTGGTTTGAATGTGATGTGATTTT
ATCCTTTGGAAGATTCCTTGGTGCTCATTACATGGAACCCATGGGCCATACCCCAGGGGTCAGTATTCCT
GTACCAAACCCCATCAAATACATAAAAATGCCTATCTCCAAACACTGCATGTCTAGACATTTACCCTGAA
GAAATACTAGCATTAGTGTGCAAAAAAAAAACAATGCATACTAGGTTGCACACAAGGATGAGTCTGACAT
ATTTTTAGATAGTAAAAATTAAAACAATCTAAATATTCAATACTGAATGAATCCTAATATAGTAAATGAT
AGAATACTGTGGTATTATTAATAAAAATGAAGTAAAACTCTTGAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4972323 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4972323 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17136 same embryo
 EMAGE:17133 same embryo
 EMAGE:17135 same embryo
 EMAGE:17134 same embryo
 EurExpress:euxassay_001519 same experiment
 MGI:4823007 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS