EMAGE mouse gene expression spatial database

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EMAGE:17188

Rnf144b ring finger protein 144B ( MGI:2384986)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001593. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001593_01	euxassay_001593_02	euxassay_001593_03	euxassay_001593_04

euxassay_001593_05	euxassay_001593_06	euxassay_001593_07	euxassay_001593_08	euxassay_001593_09

euxassay_001593_10	euxassay_001593_11	euxassay_001593_12	euxassay_001593_13	euxassay_001593_14

euxassay_001593_15	euxassay_001593_16	euxassay_001593_17	euxassay_001593_18	euxassay_001593_19

euxassay_001593_20	euxassay_001593_21	euxassay_001593_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17188_wholemount_strong.wlz

17188_wholemount_moderate.wlz

17188_wholemount_weak.wlz

17188_wholemount_possible.wlz

17188_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17188_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 02 03 04 05 06 16 17 18 19 20 21

vibrissa

weak

regional

weak expression: see section 06 19

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 10

pons ventricular layer

moderate

regional

moderate expression: see section 10 11

otic capsule

moderate

regional

moderate expression: see section 01 02 03 04 05 06 15 16 17 18 19 20 21

neural retina

strong

regional

strong expression: see section 02 03 04 05 21 22

tongue

weak

regional

weak expression: see section 09 10 11 12 13 14 15

meckel's cartilage

moderate

regional

moderate expression: see section 04 05 06 07 08 09 14 15 16 17 18 19 20

lower jaw incisor

moderate

regional

moderate expression: see section 10 11 12 13

lower jaw molar

moderate

regional

moderate expression: see section 06 07 08 09 15 16 17

oral epithelium

moderate

regional

moderate expression: see section 07 08 09 10 16 17 18

upper jaw incisor

moderate

regional

moderate expression: see section 10 13

upper jaw molar

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 14 15 16 17

bladder

moderate

regional

moderate expression: see section 11 12

basisphenoid bone

strong

regional

strong expression: see section 01 02 10 11

exoccipital bone

strong

regional

strong expression: see section 06 07 22 moderate expression: see section 19

vault of skull

strong

regional

strong expression: see section 01

frontal bone primordium

strong

regional

strong expression: see section 02

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 05 06 07 20 21 22 moderate expression: see section 18 19

viscerocranium

strong

regional

Expression in the turbinate bone.

pectoral girdle and thoracic body wall skeleton

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4786
Entity Detected:	Rnf144b, ring finger protein 144B ( MGI:2384986)
Sequence:	sense strand is shown >T4786 GAGAGAGCCCTTCCTCCGCGGTGTGCCCGCTGCCCCGGCTGGCCCAGGCTGGACCTGCTAGAGAGACGCC AAGTAGCCTGAGGACTTCAGCTCCAGCCAGGGATAGAGAAGGCTGAAGACAGGCTGATGGACTCCGTCGA TGGGCTCCAGTGTCTGACCATGACTGCGGAAAACCCACCCTCTGGAGACCTGATTCCCGCCCCGCTCGTC ACTTGCAAGCTCTGCCTGTGTGAACAGTCTCTGGACAAGATGACCATGCTCCAAGAGTGCCAGTGTATCT TTTGTACACCCTGCCTGAAACAGTATATGGTGCTGTCAATCCGAGAAGGCTGTGGATCTCCTATCACTTG TCCTGACATGGTGTGCCTAAACCACGGGACCCTGCAGGAAACCGAGATTGCCTGTTTGGTGCCTCTGGAT GAGTTTCAGCTGTATCAGCGGCTCAAGTTTGAACGAGAGGTTCACATGGACCCCCTCCGGACATGGTGTC CTGTTGCAGACTGCCAGACCGTGTGCCATATTTCAGCCGGAGACCCAGGCCAGCCAGTGTTGGTGGAGTG CCCTTTGCCACCTGAAATTCTGCTCATGTTGCAAGGATGCTTGGCACGAGGAGTCTTCCTGTAGAGACAG CCAGTCGGCGATGCCAGAGCATGGGGCTCTCTTTGGAACGGATGCGGACGCCCCCATCAAGCAGTGCCCG GTTTGCCGCATTTACATTGAGCGCAACGAAGGCTGCGCCCAGATGATGTGCAAGAACTGCAAGCACACAT TTTGCTGGTACTGTCTGCAGAACCTGGACAATGACATATTCCTCAGGCACTATGACAAAGGGCCATGCAG GAATAAGCTCGGCCACTCGAGGGCATCGGTAATGTGGAACCGAACACAGGTAGTGGGGATCCTTGTGGGA TTAGGTGTCATTGCCTTGGTGACCTCACCCTTGCTGCTCCTGGCCTCTCCCTGTATAATCTGCTGTGTCT GCAAGTCCTGTCGCGGCAAGAAGAAAAAGCATGATCCATCCACAACCTAAAGTCACCTGCGCAGACATCC GGGTCACATGAGACAGCACATTGGTAAAAGCCCCACTTAGCAGCCTTGCCTCCTTCTCCTTGCCAAACTT TGGAAGTGCCTCTGCCCAGACTTTGAACCTACCTGAAAGCATTCGGTGTTGGAAAAGGCCACGCACCGGG GCATGAGTGTGCCTGTGTATCAAGAAGCAGCCCCACAGTCCGGACTGTGTCTGTGGAGCAGGTTGGGAAC TTTGCTTTAGCCTGGGAGACTCTAAAACATCACCATCTTACCCTCCAAAGGATCCCACCAGGCTGCTCAT CTTCCATCCAAACCAAGGAGCCTGGGTGAATACTCACTGTAGTCATGGCGGTATTGCCGCACTTGGCAGC ATTGCAAACTGAGCAAACGTCTGTGTTGGTTCCACTGCCACAAGACACATGGCAGAGGCCCAGAGCCGGA GGGAAGGATGATACTGCATAATAACACGTTCTGTGAGCAGTGGAGCCGGCTGCTTATCTCAGGAGCTGAC ACACTGACTGGCATGCCTATGCTGGCTACCGCGTGTGCACTTCTTAGCGTTTCCACAGTGACTTGGCAGA AACATAGCAGCTCTCTCCCCGTGTGATCTCATCTCCAGGCAGGAGAAACTGCCGTTCAGAGGGAGTTGTC CCTTCCCAGGAAGAGGTTGTAACTTGTAAGGCAACATGGCCTCATTCAGTGTCTGTTGTTTGGCAGATGT TATTCTTCTAAGGTGAACCAGTGTTCACCACTCACAGTCAGCCAGCAGCTGTGGTCTCTAAAAAGCGTTT TCTACGGGATGAAAGGGGAAAGCTCCAAAGCTTTCCTTGTAGACAGCAGGGTCCTTTCTTGGGCTCTGTG GTCATATTCCCAACAGATCAGGAGTTGGACCAGACAGTGTGCATCAACAGTAGACACTGTAGTCCATTCA CACATATAGAGCCCATGTGACTCCAGCTGGGCACATGTGTAGATATTTAATGGATAACACAAAATAAGAT TATTTTAAAAGACCTTTTCCCTTTCTAGGACTAAAATGATATATTTATCAGCATAAGAGGGGTTTTTTTT GGGGGGGGTTGGTTTTATTTACTTAGTACACATGTGAATTTTTGTTATAAAGTTCCTAACTTACTCAAGA GGGTAGACTCAAAAGTCTTTTACAACAACCCCTAGTGCGGTTTTATGCCTCTGTAAAGTGATATTCAGGT TATTTACATACTTTCAGACACAATGTAGAGCAGCTCTTCACTCAGCCTAATTTAGTCTTGGTCTCCCAGG AGCTGTCCAGACAATCTGAGTACTTTCTGTGCTCTCAAGAATTTCGTGTGACAACAAGCAATCGGATGGA AACAGTTCAGGTGTCTTCAGTTGCTGGGATTTGTGTCACCTCAGTGTGATACATCCTGCCTGTTATACAA TTAATCAGCAAATAGGTTTTGAAAAGATGAGCATATTTTTCCTCTAGCAGTATACAGTTGCCATTAGCAA AGCAGCCCGGTGCTTCATAAGAACTGTGTGTGTTCCACGTTAACAGAGACCTCTCCTGGAAGCCGGGGAG AGTATTCAGGGCCTGTTCTGTTTCCCCCGGTAACTAGGTGACTAGATTGTTAAACTGCTGCTATTTTAGA TCTGTTATCAAAGACCTGTTATCATCAGCCTTGCCTCTGCAGTGCAATGTATCCCTAAGTGGAGATGTCT ATATGCAAGGGATCACATGTGCGGTGGAATGTATCCTAAGTGGGCATGTATGTATTCAGGGGATCACATG TGCGGTGGAATGTATCCTAAGTGGGCATGTATATATTCAGGGGATCACATGTGTACATTAGCATCTGATG TCTGTACAGAACATTCCCTGGCCACGTGCTTTTTTTAGTCACAGTTTGTATAAAACATAGAACTCTAGAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5050848 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5050848 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17190 same embryo
	EMAGE:17189 same embryo
	EurExpress:euxassay_001593 same experiment
	MGI:4827764 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source