EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:17237

Aldh1a3 aldehyde dehydrogenase family 1, subfamily A3 ( MGI:1861722)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001618. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001618_01	euxassay_001618_02	euxassay_001618_03	euxassay_001618_04

euxassay_001618_05	euxassay_001618_06	euxassay_001618_07	euxassay_001618_08	euxassay_001618_09

euxassay_001618_10	euxassay_001618_11	euxassay_001618_12	euxassay_001618_13	euxassay_001618_14

euxassay_001618_15	euxassay_001618_16	euxassay_001618_17	euxassay_001618_18	euxassay_001618_19

euxassay_001618_20	euxassay_001618_21	euxassay_001618_22	euxassay_001618_23	euxassay_001618_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17237_wholemount_strong.wlz

17237_wholemount_moderate.wlz

17237_wholemount_weak.wlz

17237_wholemount_possible.wlz

17237_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17237_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

elbow mesenchyme

strong

regional

strong expression: see section 24

forearm mesenchyme

strong

regional

strong expression: see section 24

shoulder mesenchyme

strong

regional

strong expression: see section 23 24

upper arm mesenchyme

strong

regional

strong expression: see section 23 24

hand mesenchyme

strong

regional

strong expression: see section 01 02 24 not examined expression: see section 04 05 19 20 21

foot mesenchyme

strong

regional

strong expression: see section 02 04 05 19 20 21

upper leg

strong

regional

strong expression: see section 01 02 03 04 05 20 21 22 23 24

head mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 17 18 19 20 21 22 23 24

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

telencephalon mantle layer

strong

regional

strong expression: see section 07 08 09 10 16 17 18 19 moderate expression: see section 11 12 20

cornea epithelium

strong

regional

strong expression: see section 01 02 03 21 22 23 24

neural retina

strong

regional

strong expression: see section 01 02 03 21 22 23 24

viscerocranium

strong

regional

Expression in the turbinate bone.

tongue muscle

strong

regional

strong expression: see section 08 09 10 11 12 13

stomach

strong

regional

strong expression: see section 02 03 04 05 moderate expression: see section 06 07 09 weak expression: see section 08

hindgut

moderate

regional

moderate expression: see section 13 14 15

midgut

strong

regional

strong expression: see section 16 moderate expression: see section 09 10 11 12 13 14 15 17

meckel's cartilage

strong

regional

strong expression: see section 02 03 04 05 06 07 08 12 13 14 15 16 17 18 19 20 21

lower jaw incisor

strong

regional

strong expression: see section 07 08 09 11 12 13 14

upper jaw incisor

strong

regional

strong expression: see section 08 09 10 11 12 13 14

kidney calyx

strong

regional

strong expression: see section 06 07 08 09 10 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5648
Entity Detected:	Aldh1a3, aldehyde dehydrogenase family 1, subfamily A3 ( MGI:1861722)
Sequence:	sense strand is shown >T5648 GAACCAGTTATGGCTACCACCAACGGGGCTGTGGAAAACGGACAGCCGGATGGGAAACCGCCTGCCTTGC CGCGCCCCATCCGCAACTTGGAGGTCAAGTTCACCAAGATATTTATCAACAACGACTGGCACGAATCCAA GAGTGGAAGAAAGTTTGCCACATATAACCCTTCAACACTAGAGAAAATATGTGAGGTGGAAGAAGGAGAT AAGCCCGATGTGGACAAGGCTGTGGAGGCCGCTCAAGCTGCCTTCCAGCGGGGATCCCCGTGGCGCCGGC TGGATGCACTGAGCAGAGGCCAGTTGCTGCATCAGCTGGCTGACCTTGTAGAAAGGGACCGAGCGATCCT GGCTACTCTGGAGACCATGGACACCGGCAAGCCATTCCTTCATGCCTTTTTCGTCGACCTGGAAGGCTGT ATTAAGACCTTCAGATATTTTGCCGGGTGGGCAGACAAAATCCAGGGCAGGACCATCCCCACAGATGACA ACGTTGTGTGCTTCACCAGGCATGAGCCCATCGGGGTGTGTGGGGCCATTACACCATGGAACTTCCCCCT GCTGATGCTGGCCTGGAAACTGGCTCCTGCCCTGTGCTGTGGGAACACCGTGGTCCTGAAGCCAGCTGAG CAGACCCCTCTCACGGCTCTGTACCTAGCCTCTCTCATCAAAGAGGTCGGGTTCCCTCCGGGTGTGGTGA ACATTGTACCAGGCTTTGGGCCCACTGTGGGAGCAGCAATTTCCTCCCATCCGCAGATCAACAAGATAGC CTTCACCGGCTCCACAGAGGTTGGAAAGCTGGTCAGAGAAGCCGCCTCCCGGAGCAACCTGAAGAGGGTC ACACTGGAGCTAGGAGGCAAGAACCCGTGCATCGTGTGTGCAGATGCTGACTTGGACTTGGCCGTCGAGT GTGCTCACCAGGGAGTGTTCTTCAACCAAGGCCAGTGCTGTACAGCGGCCTCCAGGGTGTTCGTGGAAGA GCAGGTCTACGGGGAGTTTGTGAGGAGGAGTGTGGAGTTCGCCAAGAAGAGGCCAGTTGGAGACCCCTTC GATGCCAAAACGGAGCAGGGGCCTCAGATCGACCAAAAGCAGTTTGACAAAATCCTCGAGCTGATTGAGA GTGGGAAGAAGGAAGGGGCCAAGCTAGAATGTGGGGGGTCAGCCATGGAGGACAGAGGGCTGTTCATCAA ACCCACGGTCTTCTCAGATGTTACGGACAACATGAGGATTGCCAAAGAGGAGATTTTCGGACCAGTGCAG CCGATCCTGAAGTTCAAAAACCTGGAGGAGGTGATCAAGAGAGCGAATAGCACCGACTATGGACTCACAG CAGCAGTGTTCACCAAAAACCTGGACAAAGCACTGAAGCTGGCTGCTGCGCTCGAGTCGGGGACAGTCTG GATCAACTGCTACAATGCATTTTATGCACAGGCTCCATTTGGTGGCTTCAAAATGTCTGGGAATGGCAGA GAACTAGGAGAATATGCTCTGGCTGAATATACAGAAGTGAAAACTGTCACCATCAAACTCGAGGAGAAGA ACCCCTGAGGAACAGGCCGGCTTCTTCCTCAAAAGCTGGACAGCTGACCGTGGCAACTCCAGCCTGCCAC GAGCAGAAAGCAGGATGACAGTGCTCAGCTTCCCAGGACCCTCTCTTCTGGACACCCGATCTACTGGAGT TCAATGGTTTTGACTTTCCTCTCACTCACACGCATGCTTTATGGACCAAACTGTGGGCAGACGGATAGGA TATGGGAAACTGGAGGGCTGCCTGGAGAGGAACTCTTACCCAGCTCTGACTTTGCCTTTAGACTAAGTGC AGGACACCGCAAAGACAGCCAGTGCTCTTAACTATGAGACAGTAGTCACTGGAGTGTCCCACGGTTAATT TAAATGCTTTTTGACCCCAGGAGGAATATAGAATGAGCTCACTAAATGTTCTGTGAGCAAATCTGGTCTT TGGGGTCTCTTTGGGGTCATCTGTGCACACAAGTCCCTCCTTGTTCTGAGGGGGAAAGTGGGATTATTCA CAACAAAGGATTAGTGTTGAGGTGAAGGGACAATTTCAGGGACCCAGTGTGGGGAGCTTAGCGAAGCTGC CATGGAAAGGCCCCAGAAGCCTTTGGTGTGCAGGGGTGCGGGTCCTAAGGGCTGCATATGGGCACTGACC ATGGGGTTCACCTTGAAAACAATCCTGACTTTGGAATGACCAAATCAACATCCCAAAGAGTTTGCCCTAT AGTTATGAGATGGTCTTCCACGACATAGGCCCATCTAAGGCAGACAACGTGTCACCTTTGCCACTGAGGG CTCATGTTAGAACTACCATGGCAGAGCAAACCCTGGGCAATGGAGCCTCACATTTGACCCTCAGAGCCAC CATTCACTTTTTCAAATACCCGTTTCTACCACATTCAATAACGGGCACGCCCACTTCCTTTTTCTCTTCT CCAGCTCCATTACCTGTTTTAGATTAGTGAAGTTCATGCCAGCAAGAAATAGCTGCCACTGCATAGTTCT GTAGTCTTCAAAATCCCGTCTGGGCTTTCATTTATGATTGGCTAACAAGTAACACCTGGATCCCACAAGG GACCACCAAGTGACCTGGGTACCTCAGTCATGTTAAAACATGTTTTCCTTTCCTCTTTGATGTATGCATA TGATGCGCAATTTCATGTTTGAACTTGGAAAACTAAAACGATCCCCATAAAGCTTTAAGGGTAGAACCAC ACTCTTTGGGTTGACTGCTGTTTGGGTAACTTTCACTGTCAGTAAACATGCACCTGCCTAATTAGATGTG AGGCTTACATTTGAGGATAAAGTTGGGCTGAGCAAGCGTGAGAATTAAGTCTTCTATCTGAACCAGTGTG AGGTAGAGAATGTGGGGCGTGAATCGGGGCTCTCAGCCCTGTCGGAGACAAGCAATATTCCATCCACCTT CTTTCCTCCCTCAACACTTATACTACCATAACTAGTTAAGTCAGGATTTGGCTTCGGACACTGAATTTTG GGTATCTCAATAACTCTAGGCACTACCCTTCACCATAGTACACTAGAGGGTTGCTATTCTTCCATTGTAC AATAATGCCTTTAATATGAAATGTTACATTATTTATAATTGGTATCATTAATGTATCTTTTTATAGCTGT AAGTACACGGAGGTAGTGTATTTAACCTTCTGTAATATACTGTACTTAGAAATGAAAATCTATATAATGT TAGATCTCACTTCTTTTAAGGTTTACCCCTGTGGTATGGTTTTTAAATCAATTGGATTGGGGATTTTGAT CTTAAGTTCAGATTGTGTGTTCAGCAATGAAAAAATAAAGTTGGACACAGAGAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6515355 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6515355 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17234 same embryo
	EMAGE:17236 same embryo
	EMAGE:17232 same embryo
	EMAGE:17235 same embryo
	EMAGE:17233 same embryo
	EurExpress:euxassay_001618 same experiment
	MGI:4823080 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source