EMAGE mouse gene expression spatial database

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EMAGE:17250

Inhba inhibin beta-A ( MGI:96570)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003294. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003294_01	euxassay_003294_02	euxassay_003294_03	euxassay_003294_04

euxassay_003294_05	euxassay_003294_06	euxassay_003294_07	euxassay_003294_08	euxassay_003294_09

euxassay_003294_10	euxassay_003294_11	euxassay_003294_12	euxassay_003294_13	euxassay_003294_14

euxassay_003294_15	euxassay_003294_16	euxassay_003294_17	euxassay_003294_18	euxassay_003294_19

euxassay_003294_20	euxassay_003294_21	euxassay_003294_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17250_wholemount_strong.wlz

17250_wholemount_moderate.wlz

17250_wholemount_weak.wlz

17250_wholemount_possible.wlz

17250_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17250_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 10 11 12 13 14 15 16 17 18 19 20 21 22 weak expression: see section 09

forelimb

moderate

regional

moderate expression: see section 01 02 03 04 05 06 22

hindlimb

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 19 20 21 22

vibrissa

strong

regional

strong expression: see section 05 06 07 08 09 10 18 19 20 21 weak expression: see section 04

cerebral cortex mantle layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 17 18 19 20 21 22 weak expression: see section 10 11 12 13 14 15 16

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 02 22

naris

strong

regional

strong expression: see section 11 12 13 14 15 16 moderate expression: see section 09 10 17 18

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 12 13 14 15 16 17 18

lower jaw incisor

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18

lower jaw molar

strong

regional

strong expression: see section 07 08 09 10 17 18

upper jaw incisor

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17

upper jaw molar

strong

regional

strong expression: see section 07 08 09 10 17 18

genital tubercle of male

strong

regional

strong expression: see section 10 11 12 13 14 15 16

trachea

moderate

regional

moderate expression: see section 12 13

nasopharynx

strong

regional

strong expression: see section 12 13 14 moderate expression: see section 15 16

axial skeleton

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14

cranium

moderate

regional

moderate expression: see section 01

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 19 20 21 22

tail skeleton

moderate

regional

moderate expression: see section 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5655
Entity Detected:	Inhba, inhibin beta-A ( MGI:96570)
Sequence:	sense strand is shown >T5655 CTGACCTCATGAGACAAGAGCCGGCTGACAAAACAGAAGGGACCCGAAAGAGAATTTGCTGAAGAGGAGA AGGAAAAAAAAAAGTCCAAAAAAACCTGTGCGTGAGGGGTGGGGAGGAAAAGCAGGGCCTTTAAAGAAGG CAACCACACGACTTTTGCTGCCAGGATGCCCTTGCTTTGGCTGAGAGGATTTCTGTTGGCAAGTTGCTGG ATTATAGTGAGGAGTTCCCCCACCCCAGGATCCGAGGGGCACGGCTCAGCCCCGGACTGCCCGTCCTGTG CGCTGGCCACCCTTCCGAAGGATGGACCTAACTCTCAGCCAGAGATGGTAGAGGCTGTCAAGAAGCACAT CTTAAACATGCTGCACTTGAAGAAGAGACCCGATGTCACCCAGCCGGTGCCCAAGGCGGCGCTTCTCAAC GCGATCAGAAAGCTTCATGTGGGTAAAGTGGGGGAGAACGGGTATGTGGAGATAGAGGACGACATTGGCA GGAGGGCCGAAATGAATGAACTCATGGAGCAGACCTCGGAGATCATCACCTTTGCCGAGTCAGGCACAGC CAGGAAGACACTGCACTTTGAGATTTCCAAGGAAGGCAGTGACCTGTCAGTAGTGGAGCGTGCAGAAGTG TGGCTCTTCCTGAAAGTCCCCAAGGCTAACAGAACCAGGACCAAAGTCACCATCCGTCTATTTCAGCAGC AGAAGCACCCACAGGGCAGCTTGGACACGGGGGATGAGGCCGAGGAAATGGGCTTAAAGGGGGAGAGGAG TGAACTGTTGCTATCAGAGAAAGTAGTTGATGCTCGGAAGAGTACCTGGCACATCTTTCCAGTGTCCAGC AGCATCCAGCGCCTGCTGGACCAGGGAAAGAGTTCCCTGGACGTGCGGATTGCTTGTGAGCAGTGCCAGG AGAGTGGTGCCAGTCTAGTGCTTCTGGGCAAGAAGAAGAAGAAAGAGGTGGATGGAGATGGGAAGAAGAA AGATGGGAGTGACGGAGGGCTGGAAGAGGAAAAGGAACAGTCACATAGACCTTTCCTCATGCTGCAGGCT AGGCAGTCCGAAGACCACCCTCATCGCAGGCGTAGGCGGGGCTTGGAGTGCGACGGCAAGGTCAACATTT GCTGTAAGAAACAGTTCTTTGTCAGCTTCAAGGACATTGGCTGGAATGACTGGATCATTGCTCCCTCTGG CTATCACGCCAATTATTGTGAGGGGGAGTGCCCAAGCCACATAGCAGGCACCTCTGGGTCCTCGCTCTCC TTCCACTCAACAGTCATTAACCACTACCGCATGAGGGGTCACAGCCCCTTTGCCAACCTTAAGTCATGCT GTGTGCCCACCAAGCTGAGACCCATGTCCATGCTGTATTACGATGATGGTCAAAACATCATCAAAAAGGA CATTCAAAACATGATTGTGGAGGAGTGTGGCTGCTCCTAGAGTCGCCAGGTCCCAGAGAAAATGGATCTA GAGAGTCCAGAGAAGACAGTGGCAAAATGAAGAAAAAAATATAAGATTTATGAACTAAACAAAACAACCA GAAAAATAGAAATAATAATAATAAAAAACCCACAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6528822 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6528822 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17249 same embryo
	EMAGE:17248 same embryo
	EurExpress:euxassay_003294 same experiment
	MGI:4825592 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source