EMAGE mouse gene expression spatial database

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EMAGE:17313

Slc40a1 solute carrier family 40 (iron-regulated transporter), member 1 ( MGI:1315204)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003910. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003910_01	euxassay_003910_02	euxassay_003910_03	euxassay_003910_04

euxassay_003910_05	euxassay_003910_06	euxassay_003910_07	euxassay_003910_08	euxassay_003910_09

euxassay_003910_10	euxassay_003910_11	euxassay_003910_12	euxassay_003910_13	euxassay_003910_14

euxassay_003910_15	euxassay_003910_16	euxassay_003910_17	euxassay_003910_18	euxassay_003910_19

euxassay_003910_20	euxassay_003910_21	euxassay_003910_22	euxassay_003910_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17313_wholemount_strong.wlz

17313_wholemount_moderate.wlz

17313_wholemount_weak.wlz

17313_wholemount_possible.wlz

17313_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17313_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 17 21 22 23 weak expression: see section 02 03 04 05 06 16 18 19 20

forearm mesenchyme

moderate

regional

moderate expression: see section 01

hand

moderate

regional

moderate expression: see section 01 02 03 04 21 22 23

foot

moderate

regional

moderate expression: see section 01 02 03 04 18 19 20 21 22 23

lower leg mesenchyme

moderate

regional

moderate expression: see section 01 02 03 18 19 20 21 22 23

brain

moderate

single cell

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

vibrissa

moderate

single cell

moderate expression: see section 06 07 08 21 22

spinal cord

moderate

single cell

moderate expression: see section 06 07 08 09 10 11 12 13 14

rectum

moderate

regional

moderate expression: see section 11

midgut

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17

liver

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

urinary system

moderate

regional

moderate expression: see section 11

bladder

moderate

regional

moderate expression: see section 11 12

metanephros

moderate

regional

moderate expression: see section 05 06 07 15 weak expression: see section 14 16

lung

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

axial skeleton cervical region

weak

regional

weak expression: see section 06 15

axial skeleton lumbar region

weak

regional

weak expression: see section 07 15

axial skeleton sacral region

weak

regional

weak expression: see section 07 08 15

axial skeleton thoracic region

weak

regional

weak expression: see section 06 07 08 09 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5708
Entity Detected:	Slc40a1, solute carrier family 40 (iron-regulated transporter), member 1 ( MGI:1315204)
Sequence:	sense strand is shown >T5708 GCGAGAGCAGGCTCGGGGTCTCCTGCGGCCGGTGGATCCTCCAACCCGCTCCCATAAGGCTTTGGCTTTC CAACTTCAGCTACAGTGTTAGCTAAGTTTGGAAAGAAGACAAAAAGAAGACCCCGTGACAGCTTTGCTGT TGTTGTTTGCCTTAGTTGTCCTTTGGGGTCTTTCGGCATAAGGCTGTTGTGCTTATAGTGGTGCTATCTT CGGTTCCTCTCACTCCTGTGAACAAGCTCCCGGGCAAGAGCAGCTAAAGCTACCAGCATCAGAACAAACA CGGGGAGAACGCCTGGTGTCATGACCAAGGCAAGAGATCAAACCCATCAGGAAGGATGCTGTGGATCCTT AGCAAACTACCTGACCTCAGCAAAATTCCTCCTCTACCTTGGCCACTCTCTCTCCACTTGGGGGGATCGG ATGTGGCACTTTGCAGTGTCTGTGTTTCTGGTGGAACTCTATGGAAACAGCCTTCTCTTGACAGCTGTCT ATGGACTGGTGGTGGCAGGCTCTGTTCTGGTCCTGGGAGCCATCATTGGTGACTGGGTGGATAAGAATGC CAGACTTAAAGTGGCCCAGACGTCACTAGTGGTTCAGAATGTGTCCGTCATCCTCTGCGGAATCATCCTG ATGATGGTTTTCCTACACAAGAATGAGCTCCTGACCATGTACCATGGATGGGTCCTTACTGTCTGCTACA TCCTGATCATCACTATTGCAAACATTGCAAATTTGGCCAGTACTGCCACTGCGATCACAATCCAAAGGGA CTGGATTGTTGTTGTGGCAGGAGAAAACAGGAGCAGATTAGCAGACATGAATGCTACCATTAGAAGGATT GACCAGCTAACCAACATCCTGGCCCCCATGGCTGTCGGCCAGATTATGACATTTGGTTCTCCAGTCATTG GCTGTGGTTTCATTTCCGGTTGGAATTTGGTGTCCATGTGTGTGGAGTACTTCTTGCTCTGGAAGGTTTA CCAGAAGACCCCTGCTCTGGCTGTAAAAGCTGCTCTCAAGGTAGAGGAGTCAGAACTGAAGCAGCTGACC TCACCTAAAGATACTGAGCCAAAACCTTTGGAGGGAACTCATCTAATGGGTGAGAAAGACTCCAACATCC GTGAACTTGAATGTGAACAAGAGCCCACCTGTGCCTCCCAGATGGCAGAGCCCTTCCGCACTTTCCGAGA TGGATGGGTCTCCTACTATAACCAGCCAGTGTTTCTGGCTGGCATGGGCCTGGCTTTCCTCTATATGACA GTCCTGGGCTTTGACTGTATCACTACAGGGTACGCCTACACTCAGGGGCTGAGTGGATCCATCCTTAGTA TTTTGATGGGAGCATCAGCAATAACTGGAATAATGGGAACTGTGGCCTTCACCTGGCTACGTCGAAAATG TGGCCTTGTTCGGACTGGTCTATTCTCAGGACTAGCCCAGCTTTCCTGTTTAATCTTGTGTGTGATCTCC GTATTCATGCCTGGAAGCCCCTTGGACCTGTCTGTTTCTCCATTTGAAGATATCCGTTCTAGGTTTGTGA ATGTGGAGCCAGTGTCCCCAACTACCAAAATACCTGAGACCGTCTTTACAACAGAAATGCATATGTCCAA CATGTCTAATGTCCATGAGATGAGTACTAAACCCATCCCCATAGTCTCTGTCAGCCTGCTGTTTGCAGGA GTCATTGCTGCTAGAATCGGTCTTTGGTCCTTTGATTTGACGGTGACACAGTTGCTGCAAGAAAATGTAA TTGAATCTGAAAGAGGCATTATCAATGGTGTGCAGAACTCCATGAACTACCTTCTTGACCTTCTGCATTT CATCATGGTCATCTTGGCCCCAAATCCTGAAGCTTTTGGCTTGCTGGTATTGATTTCAGTCTCCTTTGTG GCAATGGGACATCTTATGTATTTCCGATTTGCCCAGAAGACTCTGGGCAACCAGATTTTTGTTTGTGGTC CTGATGAAAAAGAAGTTACAGATGAAAATCAACCGAATACATCTGTTGTATAAAAATAGTTTAGCTGTGG CCCCTGTTACTAGATTGTGGAGAGCATGTGTGCTTATTTTGTACTGCAGAATCCCAATAAATGCCTGCAT TTCTCTCTGGTTTTACCACCTCTGTGCCTTAAGGGCTAGGAGCACTAACTAACCATCGCCAGCAAAGATG GTTATTTCTCCTTACATGTAAACATGGGAAAATAGGAAGAGGGAGAGGCAGTCAGCGTGTGTATAGAAAG GGCTTAATGGTAAAATGAACTTCCCTTACTCTTATAAGTATATTAAATTTTGAAGAGTGGTTGTCAGGTG AGTAAAATTATTCCTTAGCAGATATTTATTATCTACTAGAATAATAAATCAATTCACCCTAAAACTCACT CTTGTAAAAGTCTGCCCCACTTCCTTTTTTAAGATCAGTGATATTTTTCTCAGTTACATTACAGAAACAA GTTTTTCCTCCATGTATGAAGGTTATAGTTAGATAATGAGTGTTATCCTTATTAATCCCATTGAACTTAA GGAATTTATTGTATGTGGAGGATGAAATGTGTAACTAGACTTGTCCAAAAGGTTCATGGAATAACTTTTG CATTTTGACCATCAGAAAGGGCAAAATATTACAGTAATGTGTGTATGGTGGCTCTTCATAAAATGACTCT CAAAATCTGCAGAAAGAAGAAAAGTTGTAGGTTTCTAGTGTGGTACAGAAGGACTTAAGGCTTGTTATTA GTGTGATTGTAGAAACTGGAAGCTAAGGGTGAGGACCTCATGTAACCAGAGTCACTGTCATCAGCCATCA CTATCTATCTTGGTAAATGAGAGAGCTGACCTGGCACCTTACAGGCAGAAAAGCGGCCCACACTAAGAAA GGACTGCAAATCAGTGAGTCTGTATAGAACATTTTACCTTCACTGACTTGGGCCGATGTGTTTGTCATCT CTGTGTTTTTCTAGAGCAACAATAGTCACAAAAGTTCCCCTTTCAATATCCCTGAGAAGAATAAAGAAAA AACTGTTTGTAAATATTTTTTTAAAACTGTTCTGCAAGTCTTTTTGTAACATGCAGTACCAACATGGGAC ATTGCCTTAACTTTTGATGCACTTTCATGGAGACTGACTGCCATGCATTGCTGTGAGCACTTTCTTTGTC GTTCAGTTTAATCTTTTCCTTCACCTTTATTACAGTATGACATAATGATCTACTATGTTGTCAAAGCTTC ATAAAATATTTCTATATAAAATGTTTGTAGAATCTTCAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2647365 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2647365 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17314 same embryo
	EMAGE:17316 same embryo
	EMAGE:17315 same embryo
	EurExpress:euxassay_003910 same experiment
	MGI:4828228 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source