EMAGE mouse gene expression spatial database

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EMAGE:17352

Zbtb7b zinc finger and BTB domain containing 7B ( MGI:102755)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003805. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003805_01	euxassay_003805_02	euxassay_003805_03	euxassay_003805_04

euxassay_003805_05	euxassay_003805_06	euxassay_003805_07	euxassay_003805_08	euxassay_003805_09

euxassay_003805_10	euxassay_003805_11	euxassay_003805_12	euxassay_003805_13	euxassay_003805_14

euxassay_003805_15	euxassay_003805_16	euxassay_003805_17	euxassay_003805_18	euxassay_003805_19

euxassay_003805_20	euxassay_003805_21	euxassay_003805_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17352_wholemount_strong.wlz

17352_wholemount_moderate.wlz

17352_wholemount_weak.wlz

17352_wholemount_possible.wlz

17352_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17352_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 19 20 21 22

forelimb

moderate

regional

moderate expression: see section 01 02 03 04 05 21 22

hindlimb

moderate

regional

moderate expression: see section 01 02 03 04 05 06 19 20 21 22

adrenal gland

moderate

regional

moderate expression: see section 07 08 09 10 16 17

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 16 17

pancreas

moderate

spotted

moderate expression: see section 08 09 10 11

adenohypophysis

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15

physiological umbilical hernia epidermis

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

vibrissa

strong

regional

strong expression: see section 05 06 07 19 20 21 moderate expression: see section 04 22 weak expression: see section 03

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 02 21 22 not examined expression: see section 05 06 07

cornea

moderate

regional

moderate expression: see section 02 03 21 22

naris

strong

regional

strong expression: see section 10 11 16 17

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 11 12 14 15 16 17

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 11 12 15 16 moderate expression: see section 14

not examined

regional

not examined expression: see section 07

pharynx

moderate

regional

moderate expression: see section 11 12 13 14

esophagus

moderate

regional

moderate expression: see section 12 13

tongue epithelium

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17

stomach

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 not examined expression: see section 10 11

rectum

strong

regional

strong expression: see section 12

midgut

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17

lower jaw incisor

weak

regional

weak expression: see section 10 11 12

lower jaw molar

moderate

regional

moderate expression: see section 07 08 09 16 17 not examined expression: see section 14

oral epithelium

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

upper jaw molar

moderate

regional

moderate expression: see section 07 08 09 16 17 not examined expression: see section 14

metanephros

moderate

regional

moderate expression: see section 07 08 09 10 16 17 18

urethra of male

moderate

regional

moderate expression: see section 12 13

lung

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7727
Entity Detected:	Zbtb7b, zinc finger and BTB domain containing 7B ( MGI:102755)
Sequence:	sense strand is shown >T7727 CAAAGGGTGGCGGAGGAGGAGCCCCCCAGCAACGAGCAGCGAGCCACTGACCGCGGCCTTCTGACCAGGA CCGGAGCAGAGCCCCAAGCCCCCCAGCCTGGCGGGGGACGCGCTTCTTCCTACACCCTGTGCCTCTGCAG CTCCAGCGAACGGACCCAACGGCTGAAAGGAGAAGATGGGGAGCCCCGAGGATGACCTGATTGGAATCCC ATTCCCAGACCACAGCAGCGAGCTCCTGAGCTGCCTGAACGAGCAGCGCCAGCTGGGCCATCTGTGCGAC CTCACCATCAGGACGCAGGGTCTTGAGTACCGCACCCACAGGGCTGTGCTGGCTGCCTGTAGCCACTACT TCAAGAAGCTCTTTACGGAGGGAGGAGGGGGAACCGTGATGGGAACAGGGGGTGGTGGGACGGCTTCTGG GGGAGCAGGGGCCGGTGTGTGTGAGCTGGACTTTGTAGGGCCAGAGGCCCTAGGTGCCCTGCTCGAGTTT GCTTACACAGCCACACTGACCACCAGCAGCGCCAATATGCCGGCTGTACTCCAAGCTGCTCGGCTACTGG AAATCCCGTGTGTCATCGCTGCTTGCATGGAGATTCTACAAGGCAGTGGGCTGGAAGCCCCCAGCCCCGA TGAGGATGACTGTGAGCGAGCCCGACAGTACCTGGAGGCTTTTGCCACTGCCACCACCACAGCCTCAACC TCAGGAATGCCCAACGGTGAAGACAGCCCTCCACAGGTGCCCCTCCTACCGCCGCCGCCACCGCCACCTC GACCTGTTGCCCGTCGCAGCCGCAAACCCCGGAAAGCTTTTCTTCAAACCAAAGGGGCCCGAGCAAACCA TCTGGTCCCTGAGGCACCCACAGTACTCACCCATCCCTTGACCTACGAAGAAGAAGAGATGGTTGGTAGA TTGGGTAACAGTGGGGGCAGTGGGCTCGGAGATAGCTATAGTCCTCCTACAGGTGCCGCCTCACCTGCCG AGGGGCCCCTGAACTATGAAGTCTTTGAAGGTGAGGAAGAAGAGGAGGAGATGGCATACCCCCCAGGCTA CGGGCTAGCCCAGAGTAATGAGCCCTCGCTATCACCAGAGGAGCTGGGCTCAGATGAAGATCCCATCGAC CCCGACCTGATGGCTTACCTAAGTTCGCTGCACCAGGACGCCCTGACACCAGGCCTGGATGGCCAAGACA AGCTGGTGCGTAAACGCCGTTCACAGATGCCCCAAGAGTGCCCAGTCTGTCACAAGATAATCCACGGGGC AGGCAAACTGCCTCGGCACATGAGGACCCACACTGGTGAGAAGCCCTTTGCCTGTGAGGTCTGCGGCGTC CGCTTCACCAGGAATGACAAGCTGAAGATCCACATGCGGAAGCACACAGGAGAACGCCCCTACTCGTGCC CCCACTGCCCAGCCCGCTTCCTGCATAGCTACGACCTCAAGAACCACATGCACCTGCACACCGGGGACCG GCCCTATGAGTGCCACCTGTGCCACAAGGCCTTCGCCAAGGAGGACCACCTGCAGCGCCATCTCAAGGGT CAGAACTGCCTGGAGGTGCGCACCCGAAGGCGCCGCAAGGATGACGTGGCAGCCCCTCACTACCCACCGC CCTCGACCACCACCTCATCCCCTGCTGGCCTCGACCTTTCCAATGGCCACCTGGACACCTTCCACCTCTC TCTGGCTCGATTCTGGGAGCAGTCGGCCACCACGGGACCCCCAGTCACTACACAAGGGCCCCCTGAGGAG GAGGAAGAGGAAGGGACACCCACCACACCACAGGCCGAAGGTGCCATGGAGTCCTCTTAAAAGAGGGATG AATGGGCAGGCTGGAACAGCCTGGGACACCACGCCAAGCAACGGGGAGCACATAAAACAAGTGGTATCGG GGTCGGGGGTGCTGAACCTTGTTGGTATCAGAATCGAACGCCTCTTCCCCCCAGAGCCTTCATTCCAATT ACAAGCTGAGGTTTTGGGGCACAAGCTCTCTAGATTGGGGTGATCACCCAAGGAGTTAAACATGAAATAT ATGTCTGTTTGTCTGTCTGTGTGTGTATATATATATGTGTGTGTGTGTGCACGCGCGTGTGTGTGTTACA CACACTCTCTGCACACACACACATATATATACACACACAATAAAGGTGGGTCCCTGTCCCCAGCCACTAC TGGGGAATTGAAGCAATAATCTATCTCTGATTTGTGGGGTCCCACTTCGGCTATGCGGGTTTCTAGGAGG CTGGGGCTTAGGACCAAATCCTTGCTCTACTCCCATGCCCCCTGGGTTTTGGCTGTGAAGGGGTGGACTG CCCCTCCCTTCCGAGACCCCTCCTTCCTGGTTTCTGTTCCTTTTTCCTGGCCGTAAATTATGCAAAGGGG GGTGGCAAAGGAAGGGTAAGAGGGGGGAAACAAGGTAGAGGCTTGAAAGACTGGGGTGGCGGCGGTGGGG ACTGGGCCTGTAAGGACGGAGCCATCCCAGTCCCCCTCCACCCAGTTCCAACGCTGAGTCATGGGGTCCT GGAGAAGAGGAGGGCAGGGTGGCCTGATTGGCTCGCCCGCCCCTGGGGGTAGCAGAAGGTCTCCGCCCAG CTAAGGGAGCTCTCCACCTCTTCCCTCCCGCGTCCCGGGGCAGGGAATGTCTTGTTCCTGGCGCTCCCTC CCTGGGGCCAGAGGGCAGGGCGGGCCGGGCGTCTTACCCTCTTCTCCTCCCTACCTCCCCGCCCAGGTGC AAGCGGGAGCCGCCTCTGCTGCTTGCTGCCCCTGACTCACGCCGCCCCCGGGCTGGCGGGCGCTGGGTGT GGGATGGGGTGAGGGGTTGAGGTAGCCTTGTAGGGAGCGGACAAGCTAGCGCCATCTGGTGGCCATGCCC TGTAAGGGCGAGCGCTACCCCCCAACCCCACCCCCCAGCGACCACTTTTCATGAGCTAGGGTCACACCCA GCCATCTAGGCCTGTGAACGGGTGCCCCCTTGCCAACATGGTGGCTAGGCAAGGCAACTCACTTGCCCCT GGGAAAAGTGAGCTGCGGAGCAGACCCAGAGGTAAGGGAACCTTTCCTCAGACCTTGCCTGGCACTCTTA GGACTGAGTCCACCCCCGTTCTGTACATAGCCTCTTCTGATGGTCTTGTTGAAATCCAATTTCACATTTT TAACTATCAGTTTTCCTGGGGTGGGGAAGAGTCTTCACCCCACCCCTTCCTCGCTGGGTGCTGGACCCCC ATTGCGGCCTGGGCTCTGCCTTGCACTATTTACCCCTTTGGCCTAGTGGCCCTTCCCCCTCCTGGAAAGG GGCAGGTTCAGGGGCCCGGTGCTCTCCTCTCCCCATGCACCTCCATGCCCATTTGCACAGCTGCCCAGGT ACCCCCAACAGTGGGGAAGGGCCACACAGGGAGGGGTGGCGGGACCAGTCCCTGTATCTATTTAAAAAGT GATGATGTAATATATTAGGAGGGAGGTCAGGTACCCTGGGCCTCATCTTAGCATTTCAGGTGATAGGGGA GCCCAGGGCTGGAGAAACCTGGGGCTTAGTCCCAGAGAGTGGAGACAGTGGCCTCCACTCTCTCTCCTTG TGGCTTCCCCACTCAGTGCCTTAGTGGGGGAACGCATTCCCCTCCCCCATTCCCTTCATCTACCCCTGCC CCTGCCTCGGGTGTAAGTGACAGGAAGAAATAATAATAATTTAAGATTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6309645 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6309645 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17353 same embryo
	EMAGE:17354 same embryo
	EurExpress:euxassay_003805 same experiment
	MGI:4829264 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source