EMAGE mouse gene expression spatial database

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EMAGE:17410

Cep120 centrosomal protein 120 ( MGI:2147298)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003847. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003847_01	euxassay_003847_02	euxassay_003847_03	euxassay_003847_04

euxassay_003847_05	euxassay_003847_06	euxassay_003847_07	euxassay_003847_08	euxassay_003847_09

euxassay_003847_10	euxassay_003847_11	euxassay_003847_12	euxassay_003847_13	euxassay_003847_14

euxassay_003847_15	euxassay_003847_16	euxassay_003847_17	euxassay_003847_18	euxassay_003847_19

euxassay_003847_20	euxassay_003847_21	euxassay_003847_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17410_wholemount_strong.wlz

17410_wholemount_moderate.wlz

17410_wholemount_weak.wlz

17410_wholemount_possible.wlz

17410_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17410_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 15 16

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 15 16 17 18 19 20

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 16

spinal cord

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 08 14

cervical ganglion

moderate

regional

moderate expression: see section 06 07 14 15

thoracic ganglion

moderate

regional

moderate expression: see section 11 12 13 14

dorsal root ganglion

moderate

regional

moderate expression: see section 05 06 07 08 09 10 14 15

retina

moderate

regional

moderate expression: see section 01 02 03 20 21 22

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 09 10 11 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7799
Entity Detected:	Cep120, centrosomal protein 120 ( MGI:2147298)
Sequence:	sense strand is shown >T7799 CGGCGGCGTCCGCTGCGGCTGCCCGGGCGTTTGAATAGCGGCTTAGCAGGCGGCAAAAAGACCCTGCCGC CGCGACCATGGTCCCCAAGTCCGACCAACTGCTCATCGTCGTATCCATCCTCGAAGGTCGCCATTTCCCC AAACGTCCAAAGCATCTGCTCGTGGTGGAAGCAAAGTTTGATGGGGAGCAGCTGGCTACTGATCCTGTCG ACCATACTGACCAGCCAGAATTTGCTACTGAGCTAGCCTGGGAGATTGACAGGAAAGTCCTTCATCAGCA CCGGTTACAGCGCACGCCGATCAAACTCCAGTGCTTTGCCTTGGACCCGCAGACTTCTGCCAAGGAAACC GTAGGCTACATTGTTTTGGACTTAAGAACAGCTCAAGAAACAAAGCAGGCTCCCAAATGGTACCAGCTGC TGAGTAACAAATACACCAAGTTCAAGGCCGAGGTGCAGATCAGCCTCACTTTGGAGACAGATACAAAGGC CCAGGTGGATAGTTATAAAGCAAAAGCAGCCCCGCCTCGGGATGGAAAAGTTCTTGCTAGCCTGGCAGGA GTTGACCCGAAGGACATTGTTGCAGTATTGAATGAGGAAGGAGGCTACCATCAGATTGGACCAGCGGAGC ACTGTACTGACCCCTTTATTTTGTCGGTGACGATTGCATTTGCCACCCAGTTGGAACAGTTAATTCCATG TACCATGAAGCTTCCAGAAAGGCAGCCTGAGTTTTTCTTTTACTATTCTTTACTGGGAAACGATGTTACA AACGAGCCTTTCAGTGATTTGATCAACCCAAACTTTGAGCCCGAGAGGGCATCTGTTCGTATACGCAGCA GTGTGGAGATTCTGCGTGTGTACCTGGCTCTTCACTCTAAACTGCAGATCCATCTCTGCTGTGGAGACCA GTCACTTGGCAGTACAGAAATACCTTTAAATGGATTACTGAAGAAGGGCAGTACAGAGATTAACCAGCAT CCGGTCACAGTAGAAGGTGCTTTTACCCTTGACCCTCCAAACCGAGCCAAGCAGAAGCTAGCTCCAGTCC CTCTGGACCTGGCCCCAACTGTGGGCGTGTCCGTGGCTCTGCAGAGAGAAGGCATCGATTCCCAGTCTTT AATTGAATTAAAGACCCAGAATGGACATGAGGCAGAACATTCACAGAAGAGAGTTTTAACCCCCATAAAG GAGAAGACACTCACTGGGCCAAAATCACCAAGGGAGTCCCCTGCCCCGCCCCCACCTCCAAACCAGACGC CTCCAACCAAGGATGATGCAACAGAAAGTGAAGTGGAGAGCCTGCAGTATGACAAGGATCCAAAGCCCAC TGTGAAGGGCATCGGTTCTGTACCTGCTTCGCTGGCCCAGCCAGAGGCCACCTGTGGTGCGTCAGAAGTG GTGACATCAGGACAGAAGATTGCTGTTCCAGCGGCGTCACACCACTTCTGCTTCTCAGTAGACTTAAGAA GCGTCCATGACTTGGAACTCAGTTTTCCAGTCAACTGCATATTGAGGTACTCCTACCCATTCTTCGGCAG TGCAGCTCCTATTATGACTAACCCTCCCGTAGAAGTTCGGAAAAACATGGAGGTTTTTCTTCCTCAGTCT TACTGTGCGTTTGATTTTGCAACTATGCCTCACCAGCTCCAAGACACCTTCCTACGGATTCCTCTGCTGG TGGAGTTATGGCACAAGGATAAAATGAGTAAAGATTTACTCCTTGGGGTCGCAAGAATCCAGCTTTCTAA TATCTTGTCTTCAGAAAAGACCCGGTTTTTAGGTGCTAATGGTGAGCAGTGCTGGCGACAGACGTACAGT GAGAGCGTGCCTGTGATAGCAGCCCAGGGGTCAAATAACAGGATATTAGACCTTTCTTATACAATGACAC TGGAAGACTATGGCCTAGTGAAAATGCGTGAGATCTTTGTATCGGAGTCATCTCAGGGTGTGCCTGCTGT GGATCAGAAGCCATCTTCGCCTCCTCCCGCTCCCTGCCCTTCTGAGATCCAGATGGAACCCAGAGAGACC TTAGAGTACAAGGCTGCCCTCGAGCTGGAGATGTGGAAAGAGATGCAGGAAGACATATTTGAAAGTCAGC TGAAGCAAAAAGAACTGGCTCACATGCAAGCTCTTGCAGAAGAGTGGAAAAAAAGGGACCGGGAAAGAGA GTCACTAGTGAAGAAAAAGGTAGCTGAATACAGTATTCTAGAAGGCAAACTTCAGAAGGCCCTCACGGAG CTAGAGACCCGTGAGCAGCAGCTGGCCAGTGCAGAAGCAGAGCTTCAGAGAGAGAGAAAGGAGCTGCAGT TAGAACGGGAGCGGAACCTTCAAGAGCTGCAGGACTCTGTCCGAAGGGCCAGAGACGACTGTGTGTACCA AGTGGAGCTGGAGAGGCTGAAGCTGAAGCAGCTGGAAGAGGACAAACAGCGGCTTCAGCAGCAGCTTAAT GATGCTGGCAATAAGTATAAGACTTTGGAAAAAGAGTTTCAGCAGTTCAAGGATCAACAGAACAACAAAC CAGAAATCCGGCTGCAGTCAGAAATAAACCTCCTCACCTTGGAGAAGGTTGAACTTGAAAGAAAGTTGGA ATCTGCAACCAAGTCTAAACTGCATTACAAGCAGCAGTGGGGCCGAGCTTTGAAAGAACTTGCCAGACTA AAGCAGAGGGAGCAAGAAAGTCAAATGGCCCGCCTTAAAAAACAGCAGGAGGAGTTGGAGCAGATGCGGC TGCGTTACCTGGCGGCTGAGGAGAAAGAAACAGTGAGAACTGAGCAGCAGGAACTGCTGGATATAAGGAA CGAGCTGAACAGGTTAAGGCAGCAAGAACAGAACCAGTACCAGGACTGCAAAGAGATTGCAAGTGGGAAG CTGGGCAGCCCGAGGGGCTCTGGGCTGGAAGAAGGCTTGGATGACTACCTGACACGCCTGATAGAGGAAA GGGACACTCTGATGAGAACGGGTGTCTATAACCATGAGGACCGCATAATAAGTGAACTCGACCGGCAGAT CAGAGAGGTTTTGACCAAAAACAGTGCCAGTTAAAAACCACCGGGGAAACGTTTGCAGATACGCTAGGTC TGCAGTTTGATATTTTGTAAAATCCTCAAAAGCAAGGGAAGTGGCTACTTTGGAATGCTCTTTATAATTT TTATAAGCTGAATTTTATATGTTACTGTATATAGTATTTATTTGATTTTACTTACGCTACCTCTCCAATG CTGGCTTCATTTTAATTGTGTTTTATATGCTCATTTAAAATGACTTTTCAGTGTTCTTCAGTTTAAAATC TGTTGTTTGACTTTAAAAACAGCCTTTCACAACTTGTCCCAGGAGAACTGACCACAGTAGTTCCCACTGT ATAATTTTTATGTTGAGCCAAGAGAGAATATGTTGCACTTTAAACACGCTGTGTCCTGCTCCCTTTGAGT GTAGATATCTTCAAGTTGAAGCCAATTATAAGTGTAGCCATTTTTAAAAAGCATTTATATGACCAGTCTT TTTCCCCTCAACCTGTGGTATTTGCAGATGACAACTTTGAAAATAAAGCAGTCTCGGGCAGAGACGCTGT GTGACATGTTGGCTGACAAATTAGTCATTGCTTAAACACAGTTCATGCAGATTAAACACAAATCAAGTTC CAAATTTCATTGGACTCCACTTGAAGACCATGAGAAAATAGCTTGGTCTGACCACAATCTCCTGCTCATG ACTCTCTGAGGCTTCTTGTTAAAATGGTTGCCTTTGTTTCTCAGTGATAGCGATACAGCTCAGGAATGGA CTATGGAAACTGATACCTAGCGACATTAGCTGAGAACACCACGGAGCTGCTTCCCTCCCTCTGCAGTCAG TCTGTTTTCAGAGGCACCCCAGCGAGCCCAGTGCAAGCCCGTAACCCTCCAGAGCTCCTTCACACTGCGC TCTCCAAACCACCCCATCCTCACAGCGAGCTTATCCCTACCTCTGCGTGACACTGAGCAGGCAGATTAGC AATCTCCCAGGTCATGCAGCATCTTGAAACAAAGTAAGAATTCTGATTCCCAAAAATAACAAATTTAAGT GTATACTTTAGCAGGTGTGTACTAATTACCAGTAACTTCAACACAAGCAAAGCAGGTTACATGGTAATGT TCATTCAGTATATTTCTTGTTTGTGAAATTGAAACCTCTATAGGCATCATATGCTTCTTGTCTTTAACAA AGTCTTTGTCTTCTAAGAGCAACCCCGCAGTAAACAACAGAGGGGTTGCTGGACTTGAGGGTGACGAGAC AGATGATCATAGCAGGGATTTGAAATATGAATTATGGTTACATGGTCCTGATTTTTTTTTTTTGGTGACA TGAATGGAACAGAAGTATTTTGTTTTAGAAGGTCCTGGCCCAGCGTTGGTTCTTCCTCCTGTATCCTGTG AGGCCTGGAGCTTTTCAAACACTAGAGGGGGCCTTAGAGTAAGTGGAAAGGACTTCTAAAATGTGTGTGT GCTTTGCCTTCCAGAAAGCCATCTTTGGGCTACTGTAATATGACAAATAAAATCCTCACTGTGTGCATCC TTGAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6328829 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6328829 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17412 same embryo
	EMAGE:17411 same embryo
	EMAGE:17413 same embryo
	EurExpress:euxassay_003847 same experiment
	MGI:4823812 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source