EMAGE mouse gene expression spatial database

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EMAGE:17420

Nudt11 nudix (nucleoside diphosphate linked moiety X)-type motif 11 ( MGI:1930957)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003856. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003856_01	euxassay_003856_02	euxassay_003856_03	euxassay_003856_04

euxassay_003856_05	euxassay_003856_06	euxassay_003856_07	euxassay_003856_08	euxassay_003856_09

euxassay_003856_10	euxassay_003856_11	euxassay_003856_12	euxassay_003856_13	euxassay_003856_14

euxassay_003856_15	euxassay_003856_16	euxassay_003856_17	euxassay_003856_18	euxassay_003856_19

euxassay_003856_20	euxassay_003856_21	euxassay_003856_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17420_wholemount_strong.wlz

17420_wholemount_moderate.wlz

17420_wholemount_weak.wlz

17420_wholemount_possible.wlz

17420_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17420_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 16

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 15 16 17 18 19 20

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 04 05 06 15 16

spinal cord

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 06 12

cervical ganglion

moderate

regional

moderate expression: see section 05 06 13 14

thoracic ganglion

moderate

regional

moderate expression: see section 08 09 10

lower jaw incisor

moderate

regional

moderate expression: see section 11 12

lower jaw molar

moderate

regional

moderate expression: see section 05 06 18 19 weak expression: see section 07 17

upper jaw incisor

moderate

regional

moderate expression: see section 11 12

upper jaw molar

moderate

regional

moderate expression: see section 05 06 18 19 weak expression: see section 07 17

metanephros

moderate

spotted

moderate expression: see section 03 04 05 06 07 12 13 14 15 16 17

lung

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7807
Entity Detected:	Nudt11, nudix (nucleoside diphosphate linked moiety X)-type motif 11 ( MGI:1930957)
Sequence:	sense strand is shown >T7807 CCCGCCCGCCCGCCCCCGCCTCGTCTCCGCTGGCTCGGCACAGACTGCGCTCCGCTCCACTCCGCTCTCC TCTCCTCTCCTCCAGCCTCGCCGGGCCCTCTCCCTGCCAGCCTTTGCTCCTCGGCTGCGCGGCGGCAGCG GCCCGCTCGCTAGGCAGCTGCAAGCGACCTCAGAGGCGGCGGCGGCGGCGGCGGCGGCGTCGGGGAGGAT GAAGTGCAAGCCGAACCAGACGCGGACCTACGACCCCGAGGGCTTCAAGAAGCGCGCCGCGTGCCTGTGC TTCCGCAGCGAGCGCGAGGACGAGGTGCTGCTGGTGAGCAGCAGCCGCTACCCCGACCGCTGGATCGTGC CGGGAGGGGGCATGGAGCCCGAGGAGGAGCCGGACGGCGCGGCGGTGCGCGAGGTGTACGAGGAGGCGGG AGTCAAGGGGAAGTTGGGCCGCTTGCTGGGCGTCTTCGAGCAGAACCAGGACCGCAAGCACCGGACCTAC GTGTTCGTGCTCACCGTCACCGAGCTGCTGGAGGATTGGGAAGACTCGGTCAGCATCGGCAGGAAGCGCG AGTGGTTCAAGATCGAAGATGCCATCAAGGTCCTGCAGTGCCACAAGCCCGTGCACGCCGAGTACCTGGA GAAACTGAAGCTGGGCGGCTCCCCTACTAATGGGAACTCGGCCGCCCCGTCCCCGCCAGAGAGCGAGCCC TAATGAACCGAGGCATGCTCAAGATCACACTGAAATCATCATTGATGTGAACCCAGTATCCAGTGGTATT GTCAAGTTCACCGGAGCACCATTAAGGTTGCCAAGGAGACCGCTCATTCTGGTTTTCCTTCTACATCTTG GAACACTCTTCTGTCTATCTTACCAACGTTTTTTGCTCTGGTTGTTGTACTATTGTGTGTGAACGGACTT AATTCAGCACCTATAGCCTTTCGTTGTGCTTTTCTGATGTGTCTGCTTTCTTCATTACACCTTTGAGAGC AATGACTATTTTTCCTTACTCTGCATATTCTGCATCCAAGACACTACTTGAAATATGGTTGGCATCACTG GAGGTCTTTGATTCTATTAGTATTTTGTAATCTCTTTGTGGCTAAACATTTCCCTCCCAATCTGGTGCTA GTAGAGTATCCGCTGTCTAAGCACCACGTGTGTAGCTCATGTGTATTCGGTGTTGCAGAAACATTTCAGG ACAGTTTGAGATGTTCCAGAACAAGAATTAGTATTCATTTATATGTCATACCACACAATGGCTGGCATGG TTTTAATATTATGTCATCATTTAGGGTAACATTTTATAGAATTAGTCCTTCACCTAACAACTTTAGTGTT TTTGTACTGTTGCTAATTTGCTTAAAATTTTATTCAAAAGGTATCACTTGGTATAAAGGTAATTGTAACA CATTACAATGAAAACCTATTGTGAGTTTTTATAAAAAATAAATTTTAAATTAATATACAAAGAATAATAC ATTTAATTTTTTTTGCAGTGCTCTACTTCTGAAATGTCACCTTAACATTTCCTTGCAGAAAATTCTGGGG TTGTTTTTGTTGTTGTTGTTGTTGTTTACAGATTCAATATTCTGTATTATTTGATGATGAGCACGAAAAA AAAAAAACCTGCTACTGAAGCAGTTTTCAGAGCTAATTGTGCCATTTAGTTGCTTAAGTGTCTTTTGCCT CTCTCACTTTTTCAGTTGGCTGTGGTTCTCATCCCTTTCCTAGTACAGTGGTCTGGCTGGCTCCTCAGTA CCACAAGCTCTCTGCATGTTTATGCAGAGGAGAGTGAGTGCTAAATTGGTGTCCCTTAGAAAGGCTTCAG GGAAAGAGACTGCTGTAAAGGGGAATGCAAAATAAAGAGTTTAAATGAGGAAGCTCAGTTTGACTCTTTC CAGAAGAATAACTTTATGCCAAGGACAAAGACATTTCAGAGTTTTGTCACAGGGTGTGTGATGTCAACAC TCTTACTCAGCTCATCCTGAGGGTTCCAAAGAGGTTCTGATATGGCTGGTGGTTGTGTCTCGCTTTTTCA TTATAAACCATTAGATTTGGAAAGGCCTGAACTCCAATTTAGTGTTAACACCCTTCCTCATGGTACCAGT GAAATGTATTGTGTCCCCATATCTTACAGACGTTAAAATAAAACCACACATTCTAATTTGCTGGAGTTTC TATTTGTGACATGTACTATGTGGATGTGCTTTCTAAAGAAACCCTTATCTTTTAGAAAACTGTACTGAAA ATTTACAGAGATGCTACTGTTATTTGCTTCAATATAATCAGGGTGGTATACGTTTGTACATAGATGAAAC AAGACTGACTCTGACTTGATAATTGTTGAAGCTGTGTGATAGATGTTTGAGAATTCATTATTCTCTATAC TTTTGTATATGTTTGAAATTTTCCATAATAAAAATTGTAAAACTGTAAAAAAAAAAAAAAAAAAAAAAAA AAA
Notes:	The probe template was PCR amplified from IMAGE:6331160 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6331160 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17421 same embryo
	EMAGE:17418 same embryo
	EMAGE:17419 same embryo
	EurExpress:euxassay_003856 same experiment
	MGI:4826827 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source