EMAGE mouse gene expression spatial database

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EMAGE:17436

Dpt dermatopontin ( MGI:1928392)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003882. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003882_01	euxassay_003882_02	euxassay_003882_03	euxassay_003882_04

euxassay_003882_05	euxassay_003882_06	euxassay_003882_07	euxassay_003882_08	euxassay_003882_09

euxassay_003882_10	euxassay_003882_11	euxassay_003882_12	euxassay_003882_13	euxassay_003882_14

euxassay_003882_15	euxassay_003882_16	euxassay_003882_17	euxassay_003882_18	euxassay_003882_19

euxassay_003882_20	euxassay_003882_21	euxassay_003882_22	euxassay_003882_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17436_wholemount_strong.wlz

17436_wholemount_moderate.wlz

17436_wholemount_weak.wlz

17436_wholemount_possible.wlz

17436_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17436_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

weak

regional

weak expression: see section 01 02 03 05 06 16 17 18 19 20 21

cervical intervertebral disc

strong

regional

strong expression: see section 08 09 10 11 12 14

lumbar intervertebral disc

strong

regional

strong expression: see section 09 10 11 12

tail vertebral pre-cartilage condensation

strong

regional

strong expression: see section 12 moderate expression: see section 13

thoracic intervertebral disc

strong

regional

strong expression: see section 07 08 09 10 11

humerus

moderate

regional

moderate expression: see section 01 02 03 18 19 20 21

body-wall mesenchyme

strong

regional

strong expression: see section 01 06 07 14 15 16 17 18 19 20 21 22 23 moderate expression: see section 02 03 04 05

anterior abdominal wall muscle

strong

regional

strong expression: see section 06 07 14 15 16 17 18 19 20 21 22 23 moderate expression: see section 01 02 03 04 05

not examined

regional

not examined expression: see section 02 03 04

pectoral girdle and thoracic body wall muscle

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 moderate expression: see section 01 02 03 04 05

lower lip

strong

regional

strong expression: see section 03 04 05 06 07 16 17 18 19 20

upper lip

strong

regional

strong expression: see section 03 04 05 19 20

sacral vertebral cartilage condensation

strong

regional

strong expression: see section 11

not examined

regional

not examined expression: see section 07

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7277
Entity Detected:	Dpt, dermatopontin ( MGI:1928392)
Sequence:	sense strand is shown >T7277 CCCACGCGTCCGAGAAGCCGGGCAGTATGGACCTCACTCTTCTGTGGGTTCTTCTGCCACTGGTGACCAC AGCCTGGGGCCAGTATGGTGGCTACGGGTACCCATACCAGCAGTACCAGGACTACGGTGATGATGGATGG GTGAATCTTAACCGCCAAGGCTTCAGCTACCAGTGTCCCCACGGGCAAGTGGTGGTAGCTGTGAGGAGCA TCTTTAGCAAGAAGGAAGGCTCTGACAGACAATGGAACTATGCCTGCATGCCTACACCCCAAAGCCTGGG GGAGCCCACTGAGTGCTGGTGGGAGGAGATCAACAGGGCCGGCATGGAATGGTACCAGAAGTGCTCCAAC AATGGATTGGTGGCGGGGTTCCAGAGCCGCTACTTCGAATCAGTGCTGGATCGTGAGTGGCAATTTTACT GCTGCCGCTATAGCAAGAGGTGTCCATATTCCTGCTGGATGACAACAGAGTACCCAAGCCACTATGGGGA AGACATGGACATGATTTCCTATGACTACGATTTCTATATGCGAGGAGCAACAACCACTTTCTCTGCAGTG GAAAGGGATCGCCAGTGGAAGTTCATCATGTGCCGGATGACTGACTATGACTGCGAATTCGAAAACGTTT AGACTCGCCGCGTACAGAAGTCCGGGTGAAGGGTCAGGGCCCAGGTGTCCTGAGGGTGTCATATCACATA TGTTAATATTGATTAGAACATCTACACTTCTTTCTTTTGCTCTCTTTCCTGGGATGGGAGCTATGGTGTC GGCTGCCTAGGCCTTTCTCACCACGATCACACATATAAATAAACTCACAATTAAGTCATGTTTCTCCCTT TCAACACATTCCAAGACAATCATATCTAGTTGCTGATGGCAGCTTCCTTGCACAGCACATGTACAGAGCC TGCATGCTTACAGCCTGCCACCCGGAGCACCTGTTGCAGCCTTGCTGTGATTCGCACTATAACTTGAGCT TCAGATTCAGCTTCGTGAACTTGGAGAGAAAGAAGGAGCTTGAGAGAGGTGGGATAAAGGTTCTCTGTAG CCAAGTCTGTTTGAGTGACAGCACCTAGAGACCTCACCTGGGGTGGTGGGGGGTCACTCTCTGTCTTGAG GGAACACGTCTACTCTCTCCTTCCACAAAAGCCACATAGACTTAGAAGTTCAAGTAAAGATCTATGTGCT TCAGAATAGAGGGGACTTGGAGGTGAAAGGGGAAGGTGGGAGGGTTTGAGGACTGAGCTGAAAGACTTTA GGTTGAAAGAACGTCCTCTATTCAAAGATATACACCAGCAGACCCAACAGTGAGAATGAATATGAGGGCC AGGAAAACCTGTGCGAATCAGTCTCAAGACAGAAACAATGAAAGAAAGATAAGTGAATGAGATTCACATT GGGAACCTGGGGAGACAAGAGTGGTCAGAGGAGAAGGGATCTTGGGTTATCAGCAGCTTGAGACACACCC AGTATTCATATGAAGACTGTAATATCCCTAGAAACCATAGGGATGCTGCTTGCATGTCTCTGGATTGGTC CTGAAGGATAATTCTCCCTTTCCCATGGCACTTGGAAGTGTATTCTGCAGAGATGACACTGTAATATCTC TGTCTCTAGCCATTTGTACCTTCTTCTTTAAAAACAAATAAAAGTATCAATATTCACTGTGCCATAAAAA AAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4165966 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4165966 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17437 same embryo
	EurExpress:euxassay_003882 same experiment
	MGI:4824388 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source