EMAGE mouse gene expression spatial database

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EMAGE:17458

Cd47 CD47 antigen (Rh-related antigen, integrin-associated signal transducer) ( MGI:96617)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003895. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003895_01	euxassay_003895_02	euxassay_003895_03	euxassay_003895_04

euxassay_003895_05	euxassay_003895_06	euxassay_003895_07	euxassay_003895_08	euxassay_003895_09

euxassay_003895_10	euxassay_003895_11	euxassay_003895_12	euxassay_003895_13	euxassay_003895_14

euxassay_003895_15	euxassay_003895_16	euxassay_003895_17	euxassay_003895_18	euxassay_003895_19

euxassay_003895_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17458_wholemount_strong.wlz

17458_wholemount_moderate.wlz

17458_wholemount_weak.wlz

17458_wholemount_possible.wlz

17458_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17458_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 08 09 11 12 weak expression: see section 07

thalamus

strong

regional

strong expression: see section 16

thalamus mantle layer

strong

regional

strong expression: see section 07 08 09 10 13 14 15

telencephalon marginal layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 14 15 16 17 18 19 20 weak expression: see section 09 10 11 12 13

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 15 16 weak expression: see section 11 12

rest of cerebellum mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 10 11 12 13 14 15 weak expression: see section 16 17 18

rest of cerebellum marginal layer

moderate

regional

moderate expression: see section 01 02 weak expression: see section 17 18

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 04 05 14 15

trigeminal v ganglion

weak

regional

weak expression: see section 02 03 04 05 06 07 15 16 17 18 19 20

spinal cord floor plate

strong

regional

strong expression: see section 09

ventral grey horn

weak

regional

weak expression: see section 05 06 07 08 09 10 11

dorsal root ganglion

moderate

regional

moderate expression: see section 05 weak expression: see section 06 07 08 12 13 14

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 12 14 15 16 17

lower lip

weak

regional

weak expression: see section 15 16 17 18 19

upper lip

weak

regional

weak expression: see section 15 16 17 18 19 20

liver left lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 not examined expression: see section 12 13 14 15

liver right lobe

strong

regional

strong expression: see section 12 13 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7310
Entity Detected:	Cd47, CD47 antigen (Rh-related antigen, integrin-associated signal transducer) ( MGI:96617)
Sequence:	sense strand is shown >T7310 CCACGCGTCCGGCGGAGGCGGCGGCTGCTCCAGACACCTGCGGCGGCGACCCCCCGGCGGCGCGGAGATG TGGCCCTTGGCGGCGGCGCTGTTGCTGGGCTCCTGCTGCTGCGGTTCAGCTCAACTACTGTTTAGTAACG TCAACTCCATAGAGTTCACTTCATGCAATGAAACTGTGGTCATCCCTTGCATCGTCCGTAATGTGGAGGC GCAAAGCACCGAAGAAATGTTTGTGAAGTGGAAGTTGAACAAATCGTATATTTTCATCTATGATGGAAAT AAAAATAGCACTACTACAGATCAAAACTTTACCAGTGCAAAAATCTCAGTCTCAGACTTAATCAATGGCA TTGCCTCTTTGAAAATGGATAAGCGCGATGCCATGGTGGGAAACTACACTTGCGAAGTGACAGAGTTATC CAGAGAAGGCAAAACAGTTATAGAGCTGAAAAACCGCACGGCCTTCAACACTGACCAAGGATCAGCCTGT TCTTACGAGGAGGAGAAAGGAGGTTGCAAATTAGTTTCGTGGTTTTCTCCAAATGAAAAGATCCTCATTG TTATTTTCCCAATTTTGGCTATACTCCTGTTCTGGGGAAAGTTTGGTATTTTAACACTCAAATATAAATC CAGCCATACGAATAAGAGAATCATTCTGCTGCTCGTTGCCGGGCTGGTGCTCACAGTCATCGTGGTTGTT GGAGCCATCCTTCTCATCCCAGGAGAAAAGCCCGTGAAGAATGCTTCTGGACTTGGCCTCATTGTGATCT CTACGGGGATATTAATACTACTTCAGTACAATGTGTTTATGACAGCTTTTGGAATGACCTCTTTCACCAT TGCCATATTGATCACTCAAGTGCTGGGCTACGTCCTTGCTTTGGTCGGGCTGTGTCTCTGCATCATGGCA TGTGAGCCAGTGCACGGCCCCCTTTTGATTTCAGGTTTGGGGATCATAGCTCTAGCAGAACTACTTGGAT TAGTTTATATGAAGTTTGTCGCTTCCAACCAGAGGACTATCCAACCTCCTAGGAATAGGTGAAGGGAAGT GACAGACTGTAACTTGGAAGTCAGAAATGGAAGAATACAGTTGTCTAAGCACCAGGTCTTCACGACTCAC AGCTGGAAGGAACAGACAACAGTAACTGACTTCCATCCAGGAAAACATGTCACATAAATGATTACTAAGT TTATATTCAAAGCAGCTGTACTTTACATAATAAAAAAAATATGATGTGCTGTGTAACCAATTGGAATCCC ATTTTTCTATTGTTTCTACTCAACTAGGGGCAAACGTTTCAGGGGCAACTTCCAAGAATGATGCTTGTTA GATCCTAGAGTCTCTGAACACTGAGTTTAAATTGATTCCGAGTGAGACTCGCCAAGCACTAACCTGAGGG TTAGTTACCCAGAGATACCTATGAAAAACAGTGGTATCCAGCAAGCCTTAGTAAACTCAGGTTGCCAGCA GCTTTGCCACTTCCGCTGCTAGCTGAATAACAAGACTGCCACTTCTGGGTCATAGTGATAGAGACTGAAG TAGAAAAACGAATGTGGTTGGGCAAATCCCGTGTGGCCCCTCTGTGTGCTATGATATTGATGGCACTGGT GTCTTCATTCTTGGGGGTTGCCATCATTCACACACACCCCTTTGACATACAGTGCACCCCAGTTTTGAAT ACATTTTTTTTGCACCCTGTCCCGTTCTGCTACTTTGATTTGCGTTATGATATATATATATATATATAAT ACCTTTTCTCCTCTTTAAACATGGTCCTGTGACACAATAGTCAGTTGCAGAAAGGAGCCAGACTTATTCG CAAAGCACTGTGCTCAAACTCTTCAGAAAAAAAGGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4187965 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4187965 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17461 same embryo
	EMAGE:17460 same embryo
	EMAGE:17457 same embryo
	EMAGE:17459 same embryo
	EurExpress:euxassay_003895 same experiment
	MGI:4823734 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source