EMAGE mouse gene expression spatial database

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EMAGE:17627

Dct dopachrome tautomerase ( MGI:102563)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005958. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005958_01	euxassay_005958_02	euxassay_005958_03	euxassay_005958_04

euxassay_005958_05	euxassay_005958_06	euxassay_005958_07	euxassay_005958_08	euxassay_005958_09

euxassay_005958_10	euxassay_005958_11	euxassay_005958_12	euxassay_005958_13	euxassay_005958_14

euxassay_005958_15	euxassay_005958_16	euxassay_005958_17	euxassay_005958_18	euxassay_005958_19

euxassay_005958_20	euxassay_005958_21	euxassay_005958_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17627_wholemount_strong.wlz

17627_wholemount_moderate.wlz

17627_wholemount_weak.wlz

17627_wholemount_possible.wlz

17627_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17627_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

weak

regional

weak expression: see section 01 02 03 04 05 06 08 13 15 16 17 18 19 20 21 22

humerus

moderate

regional

moderate expression: see section 22 weak expression: see section 01 02 03 18 19 20 21

fibula

weak

regional

weak expression: see section 01 22

tibia

weak

regional

weak expression: see section 01 22

femur

weak

regional

weak expression: see section 02 03 04 17 18 19 20 21 22

vibrissa

strong

regional

strong expression: see section 05 06 07 08 20 21 22

telencephalon ventricular layer

strong

regional

strong expression: see section 11 12 13 15 16 17 18

extrinsic ocular muscle

strong

spotted

strong expression: see section 04 05 06 07 19 20 21

heart valve

strong

regional

strong expression: see section 09 10 11 12

axial skeleton

weak

regional

weak expression: see section 06 07 08 09 10 11 12 13 14

basioccipital bone

weak

regional

weak expression: see section 08 10 12 13

basisphenoid bone

weak

regional

weak expression: see section 08 10 12 13

exoccipital bone

weak

regional

weak expression: see section 01 02 03 16

temporal bone

weak

regional

weak expression: see section 01 02 03 18 19 20 21

orbito-sphenoid

weak

regional

weak expression: see section 01 05 06 07 08 18 19 20

viscerocranium

weak

regional

Expression in the turbinate bone.

scapula

weak

regional

weak expression: see section 01 02 18 19

pelvic girdle skeleton

weak

regional

weak expression: see section 06 07 08 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9524
Entity Detected:	Dct, dopachrome tautomerase ( MGI:102563)
Sequence:	sense strand is shown >T9524 GAACTGAGGCCATGCTTCGGATGAAATTAAATCAGTGTTAGTTGTTTGAGTCACATAAAATTGCATGTGC GTGTACACATGTGCACACGTGTAGGCTCTGTGATTTAGGTGGGAATTTTGAGAGGAGAGGAAAGGGCTAG AACTAAACCCAAAGAAAAGGAAAGAAGAGAAGAGGAAAGGAAAGAAAAAAGAAAAGGCAATTTGAGTGAG TAAAGGTTCCAGAACTCAGGAGTGGAAGACAAGGAGTAAAGTCAGACAGAAACCAGGTGGGACGCCGGGC CAGGCCTCCCAATTAAGAAGGCATGGGCCTTGTGGGATGGGGGCTTCTGCTGGGTTGTCTGGGCTGCGGA ATTCTGCTCAGAGCTCGGGCTCAGTTTCCCCGAGTCTGCATGACCTTGGATGGCGTGCTGAACAAGGAAT GCTGCCCGCCTCTGGGTCCCGAGGCAACCAACATCTGTGGATTTCTAGAGGGCAGGGGGCAGTGCGCAGA GGTGCAAACAGACACCAGACCCTGGAGTGGCCCTTATATCCTTCGAAACCAGGATGACCGTGAGCAATGG CCGAGAAAATTCTTCAACCGGACATGCAAATGCACAGGAAACTTTGCTGGTTATAATTGTGGAGGCTGCA AGTTCGGCTGGACCGGCCCCGACTGTAATCGGAAGAAGCCGGCCATCCTAAGACGGAATATCCATTCCCT GACTGCCCAGGAGAGGGAGCAGTTCTTGGGCGCCTTAGACCTGGCCAAGAAGAGTATCCATCCAGACTAC GTGATCACCACGCAACACTGGCTGGGGCTGCTCGGACCCAACGGGACCCAGCCCCAGATCGCCAACTGCA GCGTGTATGACTTTTTTGTGTGGCTCCATTATTATTCTGTTCGAGACACATTATTAGGTCCAGGACGCCC CTATAAGGCCATTGATTTCTCTCACCAAGGGCCTGCCTTTGTCACGTGGCACAGGTACCATCTGTTGTGG CTGGAAAGAGAACTCCAGAGACTCACTGGCAATGAGTCCTTTGCGTTGCCCTACTGGAACTTTGCAACCG GGAAGAACGAGTGTGACGTGTGCACAGACGAGCTGCTTGGAGCAGCAAGACAAGATGACCCAACGCTGAT TAGTCGGAACTCGAGATTCTCTACCTGGGAGATTGTGTGCGACAGCTTGGATGACTACAACCGCCGGGTC ACACTGTGTAATGGAACCTATGAAGGTTTGCTGAGAAGAAACAAAGTAGGCAGAAATAATGAGAAACTGC CAACCTTAAAAAATGTGCAAGATTGCCTGTCTCTCCAGAAGTTTGACAGCCCTCCCTTCTTCCAGAACTC TACCTTCAGCTTCAGGAATGCACTGGAAGGGTTTGATAAAGCAGACGGAACACTGGACTCTCAAGTCATG AACCTTCATAACTTGGCTCACTCCTTCCTGAATGGGACCAATGCCTTGCCACACTCAGCAGCCAACGACC CTGTGTTTGTGGTCCTCCACTCTTTTACAGACGCCATCTTTGATGAGTGGCTGAAGAGAAACAACCCTTC CACAGATGCCTGGCCTCAGGAACTGGCACCCATTGGTCACAACCGAATGTATAACATGGTCCCCTTCTTC CCACCGGTGACTAATGAGGAGCTCTTCCTAACCGCAGAGCAACTTGGCTACAATTACGCCGTTGATCTGT CAGAGGAAGAAGCTCCAGTTTGGTCCACAACTCTCTCAGTGGTCATTGGAATCCTGGGAGCTTTCGTCTT GCTCTTGGGGTTGCTGGCTTTTCTTCAATACAGAAGGCTTCGCAAAGGCTATGCGCCCTTAATGGAGACA GGTCTCAGCAGCAAGAGATACACGGAGGAAGCCTAGCATGCTCCTACCTGGCCTGACCTGGGTAGTAACT AATTACACCGTCGCTCATCTTGAGACAGGTGGAACTCTTCAGCGTGTGCTCTTTAGTAGTGATGATGATG ATGCCTTAGCAATGACAATTATCTCTAGTTGCTGCTTTGCTTATTGTACACAGACAAAATGCTTGGGTCA TTCACCACGGTCAAAGTAAGGTGTGGCTAGTTATATGTGACCTTTGATTAAAAGTCCTTATATTGAAAAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30537997 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30537997 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17632 same embryo
	EMAGE:17631 same embryo
	EMAGE:17629 same embryo
	EMAGE:17630 same embryo
	EMAGE:17628 same embryo
	EurExpress:euxassay_005958 same experiment
	MGI:4824254 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source