EMAGE mouse gene expression spatial database

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EMAGE:17774

Kbtbd5 kelch repeat and BTB (POZ) domain containing 5 ( MGI:1919580)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008935. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008935_01	euxassay_008935_02	euxassay_008935_03	euxassay_008935_04

euxassay_008935_05	euxassay_008935_06	euxassay_008935_07	euxassay_008935_08	euxassay_008935_09

euxassay_008935_10	euxassay_008935_11	euxassay_008935_12	euxassay_008935_13	euxassay_008935_14

euxassay_008935_15	euxassay_008935_16	euxassay_008935_17	euxassay_008935_18	euxassay_008935_19

euxassay_008935_20	euxassay_008935_21	euxassay_008935_22	euxassay_008935_23	euxassay_008935_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17774_wholemount_strong.wlz

17774_wholemount_moderate.wlz

17774_wholemount_weak.wlz

17774_wholemount_possible.wlz

17774_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17774_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

moderate

regional

moderate expression: see section 01 02 03 04 05 06 20 21 22 23 24

upper leg muscle

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 19 20 21 22

diaphragm

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

forearm rest of mesenchyme

moderate

regional

moderate expression: see section 01 24

hand

moderate

regional

moderate expression: see section 01 02 03 04

foot

moderate

regional

moderate expression: see section 07 08

lower leg rest of mesenchyme

moderate

regional

moderate expression: see section 01 03 04 22 23 24 weak expression: see section 02

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

eye skeletal muscle

moderate

regional

moderate expression: see section 01 02 03 04 05 21 22 23 24

tongue muscle

strong

regional

strong expression: see section 13 14 15 moderate expression: see section 10 11 12

tail paraxial mesenchyme

moderate

regional

moderate expression: see section 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35475
Entity Detected:	Kbtbd5, kelch repeat and BTB (POZ) domain containing 5 ( MGI:1919580)
Sequence:	sense strand is shown >T35475 ACACAGACTTGCTGAGTTCCAGGGAGCCGCACCATGACGCTGGGCTTGGAGCAGGCGGAGGAACAGCGTC TGTACCAGCAGACGCTCCTGCAAGACGGCCTCAAGGACATGCTGGACCACGGCAAGTTCCTAGACTGTGT AGTGCGCGTGGGTGAGCGCGAGTTCCCGTGCCACCGCCTGGTGCTAGCCGCCTGTAGCCCCTACTTCCGC GCGCGCTTCCTGGCTGAGCCAGATAGCGCGGGAGAGGTACGCCTGGAGGAGGTGTCGCCAGACGTAGTGT CCCAGGTGCTGCACTACCTGTACACATCAGAGATCGCGCTGGATGAGGCAAGCGTGCAAGACCTGTTTGC CGCGGCGCATCGATTCCAGATCCCGTCCATCTTCACCATCTGCGTGTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 82321. Forward Primer - name:082321_F_cDNA_Kbtbd5, sequence:ACACAGACTTGCTGAGTTCCAG; Reverse Primer - name:082321_N_SP6_cDNA_Kbtbd5, sequence:GACACGCAGATGGTGAAGATG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17776 same embryo
	EMAGE:17773 same embryo
	EMAGE:17772 same embryo
	EMAGE:17775 same embryo
	EurExpress:euxassay_008935 same experiment
	MGI:4825694 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source