EMAGE mouse gene expression spatial database

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EMAGE:17934

Dlg2 discs, large homolog 2 (Drosophila) ( MGI:1344351)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011686. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011686_01	euxassay_011686_02	euxassay_011686_03	euxassay_011686_04

euxassay_011686_05	euxassay_011686_06	euxassay_011686_07	euxassay_011686_08	euxassay_011686_09

euxassay_011686_10	euxassay_011686_11	euxassay_011686_12	euxassay_011686_13	euxassay_011686_14

euxassay_011686_15	euxassay_011686_16	euxassay_011686_17	euxassay_011686_18	euxassay_011686_19

euxassay_011686_20	euxassay_011686_21	euxassay_011686_22	euxassay_011686_23	euxassay_011686_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17934_wholemount_strong.wlz

17934_wholemount_moderate.wlz

17934_wholemount_weak.wlz

17934_wholemount_possible.wlz

17934_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17934_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

moderate

single cell

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

olfactory cortex marginal layer

moderate

single cell

moderate expression: see section 10 11 12 13

medulla oblongata basal plate mantle layer

moderate

single cell

moderate expression: see section 08 09 10 11 12 14 15 16 17

medulla oblongata basal plate marginal layer

moderate

single cell

moderate expression: see section 13

pons mantle layer

moderate

single cell

moderate expression: see section 08 09 10 14 15 16 17 18 19

midbrain mantle layer

moderate

single cell

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

trigeminal v nerve

strong

regional

strong expression: see section 10 17 moderate expression: see section 09 weak expression: see section 16

ventral grey horn

moderate

single cell

moderate expression: see section 08 09 10 11 12 13 14

cervico-thoracic ganglion

weak

regional

weak expression: see section 08 09 14

cervical ganglion

weak

regional

weak expression: see section 08 15

thoracic ganglion

weak

regional

weak expression: see section 12 13

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15

mandible

weak

regional

weak expression: see section 05 06 07 08 19 20 21

maxilla

weak

regional

weak expression: see section 07 08 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37676
Entity Detected:	Dlg2, discs, large homolog 2 (Drosophila) ( MGI:1344351)
Sequence:	sense strand is shown >T37676 TTGCAAGTAGGAGACAGACTGCTAATGGTAAATAACTATAGTTTAGAAGAAGTTACACACGAAGAGGCTG TAGCGATATTGAAGAACACATCTGATGTTGTTTATCTAAAAGTTGGCAAACCCACCACTATTTATATGAC TGATCCTTATGGGCCACCGGATATTACTCACTCTTATTCTCCACCGACGGAAAATCATCTACTGTCTGGC AACAATGGCACATTAGAATACAAGACTTCCCTGCCGCCCATCCCTCCAGGAAGGTACTCACCAATTCCCA AGCACATGCTGGGTGAAGATGACTACACCAGGCCGCCGGAACCTGTTTACAGCACTGTGAATAAACTGTG TGATAAGCCTGCTTCTCCCAGGCACTATTCCCCTGTTGAGTGTGACAAAAGCTTCCT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 90521. Forward Primer - name:090521_F_cDNA_Dlgh2, sequence:TTGCAAGTAGGAGACAGACTGC; Reverse Primer - name:090521_N_SP6_cDNA_Dlgh2, sequence:AGGAAGCTTTTGTCACACTCA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17933 same embryo
	EMAGE:17936 same embryo
	EMAGE:17932 same embryo
	EMAGE:17935 same embryo
	EurExpress:euxassay_011686 same experiment
	MGI:4824321 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source