EMAGE mouse gene expression spatial database

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EMAGE:17952

Thbs4 thrombospondin 4 ( MGI:1101779)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011724. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011724_01	euxassay_011724_02	euxassay_011724_03	euxassay_011724_04

euxassay_011724_06	euxassay_011724_07	euxassay_011724_08	euxassay_011724_09	euxassay_011724_10

euxassay_011724_11	euxassay_011724_12	euxassay_011724_13	euxassay_011724_14	euxassay_011724_15

euxassay_011724_16	euxassay_011724_17	euxassay_011724_18	euxassay_011724_19	euxassay_011724_20

euxassay_011724_21	euxassay_011724_22	euxassay_011724_23	euxassay_011724_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17952_wholemount_strong.wlz

17952_wholemount_moderate.wlz

17952_wholemount_weak.wlz

17952_wholemount_possible.wlz

17952_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17952_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

strong

regional

strong expression: see section 01 02 03 04 05 20 21 22 23 24

upper leg muscle

strong

regional

strong expression: see section 02 03 04 05 06 07 08 19 20 21 22 23 24

forearm rest of mesenchyme

strong

regional

strong expression: see section 01 02

lower leg rest of mesenchyme

strong

regional

strong expression: see section 01 02 03 04 22 23 24

axial muscle

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

cranial muscle

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

pectoral girdle and thoracic body wall muscle

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

diencephalon meninges

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18 weak expression: see section 07 08 09

telencephalon meninges

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18 19 20 weak expression: see section 08 09

midbrain meninges

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18 weak expression: see section 04 05 06 07 08 09

endocardial tissue

weak

regional

weak expression: see section 07 08 09 10 11 12 13 14 15 16

esophagus

strong

regional

strong expression: see section 11 12

tongue muscle

strong

regional

strong expression: see section 10 11 12 13 14 15 16

midgut

moderate

regional

moderate expression: see section 13 14 15 16 17

tail paraxial mesenchyme

strong

regional

strong expression: see section 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37135
Entity Detected:	Thbs4, thrombospondin 4 ( MGI:1101779)
Sequence:	sense strand is shown >T37135 GTTACCCTGATGAAGAACTGCCATGCTCTGCCAGGAACTGCAAGAAGGATAACTGCAAGTATGTGCCAAA CTCTGGCCAGGAAGATGCAGACAGAGATGGCATCGGAGACGCGTGTGATGAGGATGCAGATGGAGATGGG ATCTTGAATGAACAGGACAACTGTGTCCTGACTCACAATATAGACCAAAGGAACAGTGATAAAGATATCT TTGGGGATGCCTGTGACAACTGCCGGATGGTCCTGAATAATGACCAGAAGGATACTGACGGGGATGGGAG AGGAGACGCCTGTGATGACGACATGGATGGAGATGGAATAAAAAACATTTTGGACAACTGTCCCAGAGTT CCCAACCGTGACCAGCAGGATCGGGATGGTGATGATGTCGGAGACGCTTGTGACAGCTGTCCTGATGTCA GTAACCCTAACCAGTCGGATGTGGATAACGATCTGGTTGGGGACTCCTGTGACACCAACCAAGACAGTGA CGGGGATGGCCACCAGGACAGCACAGACAACTGCCCCACAGTCATTAACAGCTCCCAGCTGGACACTGAC AAAGATGGGATCGGCGATGAATGTGATGACGATGACGATAACGATGGCATCCCAGACCTGGTGCCTCCTG GCCCAGACAACTGCAGGCTCGTCCCCAACCCAGCCCAGGAAGATAGCAACAAT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 64255. Forward Primer - name:064255_F_cDNA_Thbs4, sequence:GTTACCCTGATGAAGAACTGCC; Reverse Primer - name:064255_N_SP6_cDNA_Thbs4, sequence:ATTGTTGCTATCTTCCTGGGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17950 same embryo
	EMAGE:17951 same embryo
	EMAGE:17948 same embryo
	EMAGE:17949 same embryo
	EurExpress:euxassay_011724 same experiment
	MGI:4828695 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source