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EMAGE:18137

Ets2 E26 avian leukemia oncogene 2, 3' domain ( MGI:95456)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18137 EMAGE:18137 EMAGE:18137 EMAGE:18137 EMAGE:18137
"Pseudo-wholemount" of euxassay_011879. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011879_01 euxassay_011879_02 euxassay_011879_03 euxassay_011879_04
EMAGE:18137 EMAGE:18137 EMAGE:18137 EMAGE:18137 EMAGE:18137
euxassay_011879_05 euxassay_011879_06 euxassay_011879_07 euxassay_011879_08 euxassay_011879_09
EMAGE:18137 EMAGE:18137 EMAGE:18137 EMAGE:18137 EMAGE:18137
euxassay_011879_10 euxassay_011879_11 euxassay_011879_12 euxassay_011879_13 euxassay_011879_14
EMAGE:18137 EMAGE:18137 EMAGE:18137 EMAGE:18137 EMAGE:18137
euxassay_011879_15 euxassay_011879_16 euxassay_011879_17 euxassay_011879_18 euxassay_011879_19
EMAGE:18137
euxassay_011879_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18137Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18137_wholemount_strong.wlz
18137_wholemount_moderate.wlz
18137_wholemount_weak.wlz
18137_wholemount_possible.wlz
18137_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18137_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
rib
weak weak
regionalweak expression: see section 06 13 14 15 16 17 18 19
humerus
moderate moderate
regionalmoderate expression: see section 17 weak expression: see section 01 02 03 04 16 18 19 20
hip
weak weak
regionalweak expression: see section 07 12
fibula
weak weak
regionalweak expression: see section 01 20
tibia
weak weak
regionalweak expression: see section 01 02 20
femur
moderate moderate
regionalmoderate expression: see section 04 15 17 weak expression: see section 02 03 05 14
otic capsule
weak weak
regionalweak expression: see section 06 07 12 13
nasal septum
weak weak
regionalweak expression: see section 07 08 09 10
meckel's cartilage
weak weak
regionalweak expression: see section 06 09 10 14
axial skeleton
weak weak
regionalweak expression: see section 06 07 08 09 10 11 13
basioccipital bone
weak weak
regionalweak expression: see section 11
basisphenoid bone
weak weak
regionalweak expression: see section 08 09 11
exoccipital bone
moderate moderate
regionalmoderate expression: see section 01 02 16 17 18 weak expression: see section 03 04 05 14 15
temporal bone petrous part
moderate moderate
regionalmoderate expression: see section 01 02 16 17 18 weak expression: see section 03 04
vault of skull
moderate moderate
regionalmoderate expression: see section 18
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 01 02 16 17 18 weak expression: see section 03 04 05 13 14
viscerocranium
weak weak
regionalExpression in the turbinate bone.
scapula
weak weak
regionalweak expression: see section 03 04 16 17 18
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30009
Entity Detected:Ets2, E26 avian leukemia oncogene 2, 3' domain ( MGI:95456)
Sequence:sense strand is shown

>T30009
GGTACCCCTCGGGAGAGAGCCACCAAGCAGCAGTGGCCTTTCATCCACGCCTCTTCCACAAGCCAATGCC
CCATGGTGGAAACCGGCATAGCCTCTCTGTCCTGTATGAGACCCCCCTGAAGGGAAGAGTCTCTGGGAAG
CCACCGTGGCACCGAGCAGGCCCTGTGCATTGTGGCTATTTGAGATGCTCCAGGGAGCCAGCATGTGGTG
GATACACAGACACTGAGAAACATTGTTTTTCCTTTGGCCTTTTGCCTCCAGGAGTAGCAAATGCAAAAAG
CCACCTCTGCTTGAGAGGGCCCGGCAGGTGGAGAGTGTGATTGTCCATTTCAGAAGTCAGTTTGTTTGTA
CATACTATTGTATCTTATAATTGTCCTAATCCTCCAACAGTTCTATTTAACAGAAATTGTATATTGTATT
TAAAGTAATTATAACTTGTCTGAATCAAGAATGCAGATACCTAATAGGTTATCCTTTTATTTTTTTCACT
CCTACATAGGAACGTCATTTTACACCGATTTAATCTCTTAGAATCCAACTGAGAGAAACCGTGATATTCT
GTTTTTGTTTTATTTACCAGCAGGAATGGGCTCTGAAGGGTGACCTGGTTTTTTCTTTTGTTTTGCTCTC
TTGTTATTGTTGCTCATTATCTGACCAGGACTTTGTATATTGGTTTTTTTTTATAAGAAACTTAATGTTA
TTATTTGAGGGTCCATATGGCCTCTCCACTCTCCTGGACTGTAAAAGCTGTAAAGCGGTATTTTTCTTGA
CACATGGCACATGTTTTCCACTTCCCTGCATGCCTTAAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3486021), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 13654. Forward Primer - name:013654_F_IRAV12-15_C11_Ets2, sequence:GGTACCCCTCGGGAGAGA; Reverse Primer - name:013654_R_SP6_IRAV12-15_C11_Ets2, sequence:GCTTAAGGCATGCAGGGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18140 same embryo
 EMAGE:18135 same embryo
 EMAGE:18139 same embryo
 EMAGE:18138 same embryo
 EMAGE:18136 same embryo
 EurExpress:euxassay_011879 same experiment
 MGI:4824634 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS