EMAGE mouse gene expression spatial database

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EMAGE:18173

Itpr3 inositol 1,4,5-triphosphate receptor 3 ( MGI:96624)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011900. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011900_01	euxassay_011900_02	euxassay_011900_03	euxassay_011900_04

euxassay_011900_05	euxassay_011900_06	euxassay_011900_07	euxassay_011900_08	euxassay_011900_09

euxassay_011900_10	euxassay_011900_11	euxassay_011900_12	euxassay_011900_13	euxassay_011900_14

euxassay_011900_15	euxassay_011900_16	euxassay_011900_17	euxassay_011900_18	euxassay_011900_19

euxassay_011900_20	euxassay_011900_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18173_wholemount_strong.wlz

18173_wholemount_moderate.wlz

18173_wholemount_weak.wlz

18173_wholemount_possible.wlz

18173_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18173_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 12 13 14 15 16 17 18 19 20 21

humerus

moderate

homogeneous

moderate expression: see section 01 02 03 04 17 18 19 20 21

forelimb digit 2 phalanx

moderate

homogeneous

moderate expression: see section 01 02

hindlimb digit 2 phalanx

moderate

homogeneous

moderate expression: see section 05

hindlimb digit 3 phalanx

moderate

homogeneous

moderate expression: see section 05 06

hindlimb digit 4 phalanx

moderate

homogeneous

moderate expression: see section 05 06

hindlimb digit 5 phalanx

moderate

homogeneous

moderate expression: see section 06

fibula

moderate

homogeneous

moderate expression: see section 01 02

tibia

moderate

homogeneous

moderate expression: see section 01 02 03

femur

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 14 15 16 17 18 19

otic capsule

moderate

homogeneous

moderate expression: see section 04 05 06 07 08 14 15 16 17 18 19

nasal septum

moderate

homogeneous

moderate expression: see section 13

meckel's cartilage

moderate

homogeneous

moderate expression: see section 03 04 05 07 08 09 10 11 12 13 14 15 16 17 18 19

axial skeleton

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14

basioccipital bone

moderate

homogeneous

moderate expression: see section 04 05 15 16 17

basisphenoid bone

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14

temporal bone petrous part

moderate

homogeneous

moderate expression: see section 01 02 03 18 19 20 21

vault of skull

moderate

homogeneous

moderate expression: see section 01 02 03 04 05

orbito-sphenoid

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 09 16 17 18 19

viscerocranium

moderate

homogeneous

Expression in the turbinate bone.

scapula

moderate

homogeneous

moderate expression: see section 01 02 17 18

pelvic girdle skeleton

moderate

homogeneous

moderate expression: see section 05 06 07 08 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30043
Entity Detected:	Itpr3, inositol 1,4,5-triphosphate receptor 3 ( MGI:96624)
Sequence:	sense strand is shown >T30043 ACGAGATGGAGTGGCAGCGCCGGCTTCGCAGCATGCCGCTCATCTACTGGTTCTCCCGCCGCATGACGCT GTGGGGCAGCATCTCCTTCAACCTGGCTGTGTTCATCAATATCATCATTGCCTTCTTCTACCCCTATGTG GAGGGCGCTTCCACAGGCGTGCTGGGCTCGCCGCTCATCTCGCTACTCTTCTGGATCCTCATCTGCTTCT CCATCGCTGCACTGTTCACCAAGCGCTACAGCGTCCGTCCCCTCATCGTGGCGCTGATCCTTCGGTCCAT CTACTACCTGGGCATTGGGCCCACGCTCAACATCCTGGGTGCTCTCAATCTGACCAACAAGATCGTGTTT GTGGTGAGCTTCGTGGGCAACCGTGGCACCTTCATCCGAGGCTATAAGGCCATGGTCATGGACATGGAGT TCCTGTACCATGTGGGCTACATCCTGACAAGCGTCCTGGGCCTCTTTGCCCATGAGCTCTTCTACAGCAT CCTGCTCTTTGACCTCATCTACCGAGAGGAGACGCTGTTTAATGTCATCAAGAGTGTGACCCGCAATGGC CGCTCCATCCTACTGACCGCCCTGCTGGCCCTCATCCTTGTGTACCTCTTCTCCATCGTGGGCTTCCTCT TCCTCAAGGACGACTTCATCCTGGAGGTGGACCGGCTGCCCGGGAACCACTCCAGAGCCAGCCCCCTGGG GATGCCACACGGAGCTGCCACATTTATGGGCACATGTAGTGGGGACAAGATGGACTGTGTCTCTGAGGTC TCAGTGCCTGAGATCCTAGAAGAGGACGAGGAGCCCGATAGCACAGAGCGGGCCTGTGACACTCTACTTA TGTGTATCGTGACCGTCATGAACCACGGACTGAGGAACGGTGGTGGCGTGGGCGACATCCTCCGCAAGCC CTCCAAAGATGAGTCGCTCTTCCCAGCCAGAGTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3600031), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 16265. Forward Primer - name:016265_F_IRAV16-19_F12_Itpr3, sequence:ACGAGATGGAGTGGCAGC; Reverse Primer - name:016265_R_SP6_IRAV16-19_F12_Itpr3, sequence:CCACTCTGGCTGGGAAGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18172 same embryo
	EMAGE:18171 same embryo
	EMAGE:18169 same embryo
	EMAGE:18170 same embryo
	EurExpress:euxassay_011900 same experiment
	MGI:4825667 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source