EMAGE mouse gene expression spatial database

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EMAGE:18221

Rit1 Ras-like without CAAX 1 ( MGI:108053)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012013. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012013_01	euxassay_012013_02	euxassay_012013_03	euxassay_012013_04

euxassay_012013_05	euxassay_012013_06	euxassay_012013_07	euxassay_012013_08	euxassay_012013_09

euxassay_012013_10	euxassay_012013_11	euxassay_012013_12	euxassay_012013_13	euxassay_012013_14

euxassay_012013_15	euxassay_012013_16	euxassay_012013_17	euxassay_012013_18	euxassay_012013_19

euxassay_012013_20	euxassay_012013_21	euxassay_012013_22	euxassay_012013_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18221_wholemount_strong.wlz

18221_wholemount_moderate.wlz

18221_wholemount_weak.wlz

18221_wholemount_possible.wlz

18221_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18221_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

weak

regional

weak expression: see section 02 03 04 05 06 07 08 18 19 20 21 22

humerus

weak

regional

weak expression: see section 01 02 03 04 21 22 23

hip

weak

regional

weak expression: see section 09 10 16

fibula

weak

regional

weak expression: see section 01 02

tibia

weak

regional

weak expression: see section 01 02 23

femur

weak

regional

weak expression: see section 03 04 05 06 07 19 20 21 22 23

meckel's cartilage

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

basioccipital bone

weak

regional

weak expression: see section 08 10 13 14

basisphenoid bone

weak

regional

weak expression: see section 10 13 14

exoccipital bone

weak

regional

weak expression: see section 02 03 04

temporal bone petrous part

weak

regional

weak expression: see section 01 02 03 04 19 20 21

orbito-sphenoid

weak

regional

weak expression: see section 01 02 03 04 05 06 07 18 19 20 21

viscerocranium

weak

regional

Expression in the turbinate bone.

scapula

weak

regional

weak expression: see section 03 04 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30086
Entity Detected:	Rit1, Ras-like without CAAX 1 ( MGI:108053)
Sequence:	sense strand is shown >T30086 TCAGAGGAGGCCTTTCCCAGGGGCCCAGGACTGCCCCGGAGGACAATGGAGTCCGGAGCTCGCCCCATTG GTAGCAGCTGTAGCAGCCCTGCAGCACTCTCCCGGGAATACAAACTAGTGATGCTGGGTGCCGGTGGAGT TGGGAAGAGTGCCATGACAATGCAGTTCATCAGCCACCGATTCCCAGAAGACCATGACCCCACCATTGAA GATGCTTATAAGATCCGGATCCGCATTGATGATGAACCTGCCAATCTGGACATCCTGGACACAGCAGGAC AGGCAGAGTTTACAGCCATGCGGGATCAGTATATGAGGGCAGGAGAAGGATTCATCATCTGTTACTCCAT CACGGATCGTCGAAGTTTCCACGAAGTTCGGGAGTTTAAACAACTGATTTACCGGGTCCGACGCACCGAT GATACACCGGTGGTTCTTGTGGGGAACAAGTCTGACCTAAAGCAGCTGAGGCAGGTCTCCAAGGAAGAGG GATTGTCTCTGGCTCGAGAATTCAGTTGTCCCTTTTTTGAGACCTCAGCTGCCTACCGTTACTACATCGA CGACGTTTTCCACGCCCTCGTCCGGGAGATCCGTAAGAAAGAGAAGGAGCTAGTACTGGCCATGGAGAAG AAGGCCAAACCCAAGAACAGCGTATGGAAGAGGCTGAAGTCACCGTTCAGGAGGAAGAAAGACTCGGTCA CCTGAGTGAAGTGGGCTGTGCTCCTGTGAACTGCAGTGCTCCTTGTGCCTGCTGTGAACTGCAGTGCTCC CCAGTGCAGTCCTGTAATCTGCAGCAGGGAGCACGTCCTCTGCTCTCTGCTATGTGGTTATTCTAAACGT CACTGGCCAGGGGACTCGCCTACTGAGAGCTGTCATTGACTTACTGTCTAAAGCCCTGTGCTCCGTGACT CCCACGCATGAACTCATCAGGGCCGTGTCTACTCCTTAATTGTCACATTAGAATTTGGTCGGCCATTGTT TTGGT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4023732), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 17140. Forward Primer - name:017140_F_IRAV20-23_J21_Rit1, sequence:TCAGAGGAGGCCTTTCCC; Reverse Primer - name:017140_R_SP6_IRAV20-23_J21_Rit1, sequence:AACCAAAACAATGGCCGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18225 same embryo
	EMAGE:18222 same embryo
	EMAGE:18224 same embryo
	EMAGE:18223 same embryo
	EurExpress:euxassay_012013 same experiment
	MGI:4827749 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source