EMAGE mouse gene expression spatial database

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EMAGE:18258

Fosl2 fos-like antigen 2 ( MGI:102858)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012044. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012044_01	euxassay_012044_02	euxassay_012044_03	euxassay_012044_04

euxassay_012044_05	euxassay_012044_06	euxassay_012044_07	euxassay_012044_08	euxassay_012044_09

euxassay_012044_10	euxassay_012044_11	euxassay_012044_12	euxassay_012044_13	euxassay_012044_14

euxassay_012044_15	euxassay_012044_16	euxassay_012044_17	euxassay_012044_18	euxassay_012044_19

euxassay_012044_20	euxassay_012044_21	euxassay_012044_22	euxassay_012044_23	euxassay_012044_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18258_wholemount_strong.wlz

18258_wholemount_moderate.wlz

18258_wholemount_weak.wlz

18258_wholemount_possible.wlz

18258_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18258_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cervical intervertebral disc

moderate

regional

moderate expression: see section 11 14 15

lumbar intervertebral disc

moderate

regional

moderate expression: see section 13 14 15 16

thoracic intervertebral disc

moderate

regional

moderate expression: see section 13 14 15 16

adrenal gland

strong

regional

strong expression: see section 10 11 18 19 20 moderate expression: see section 09

vibrissa

moderate

regional

moderate expression: see section 24 weak expression: see section 04 05 06 07 08 19 20 21 22 23

cerebral cortex marginal layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

telencephalon mantle layer

strong

regional

strong expression: see section 02 03 05 21 22 23 24 moderate expression: see section 06 07 20

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 16 weak expression: see section 10 17

stomach fundus

strong

regional

strong expression: see section 04 05 06 07 moderate expression: see section 08 09 10 11

hindgut

moderate

regional

moderate expression: see section 14

midgut

moderate

regional

moderate expression: see section 14 weak expression: see section 15 16 17 18

mandible

moderate

regional

moderate expression: see section 04 weak expression: see section 05 07 08 20 21 22 23

maxilla

weak

regional

weak expression: see section 07 08 20

kidney calyx

strong

regional

strong expression: see section 09 12 17 18 19 20 21 22

kidney pelvis

strong

regional

strong expression: see section 10 11

male reproductive system

moderate

regional

moderate expression: see section 16

testis

weak

regional

weak expression: see section 10 11 12 20 21 22

urethra of male

moderate

regional

moderate expression: see section 13 14

sacral vertebral cartilage condensation

moderate

regional

moderate expression: see section 13 14 15

clavicle

moderate

regional

moderate expression: see section 09 10 14 16 17

axial skeleton tail region

moderate

regional

moderate expression: see section 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9492
Entity Detected:	Fosl2, fos-like antigen 2 ( MGI:102858)
Sequence:	sense strand is shown >T9492 CCCGAGTGTCGCTGCCTCCTGTCTGCCTTGGTTAGGGGACATCCCGGGAGCGAAAAGCCCAGCCCGTGGC CGGGCCGGTGGACCAGCGAGCGCGCAGCAGACTGTGCGCGGCCGCGGCGAGGGCGGGGAAGAAAACCACC CTGTTTCCTCTCCGGCCCCCACCGCGGATCATGTACCAGGATTATCCCGGGAACTTTGACACCTCGTCCC GGGGCAGCAGCGGCTCTCCTGCGCACGCCGAGTCCTACTCCAGCGGTGGCGGCGGCCAGCAGAAGTTCCG GGTAGATATGCCTGGCTCGGGCAGTGCCTTCATCCCCACAATCAACGCCATCACCACCAGCCAGGACCTG CAGTGGATGGTACAGCCCACAGTGATCACCTCCATGTCCAATCCCTATCCACGCTCACATCCCTACAGTC CCCTGCCAGGCCTGGCTTCAGTCCCTGGGCACATGGCTCTCCCCAGACCTGGAGTGATCAAGACCATCGG TACCACCGTGGGCCGCAGAAGGAGAGATGAGCAGCTGTCTCCTGAGGAGGAGGAGAAGCGTCGAATCCGG AGGGAGAGAAACAAGCTAGCTGCAGCCAAGTGTCGGAACCGTCGCCGGGAGCTGACAGAGAAGCTGCAGG CGGAGACCGAGGAGCTGGAAGAGGAGAAGTCTGGGCTGCAGAAAGAGATTGCTGAGCTGCAGAAGGAGAA GGAGAAGCTAGAGTTCATGTTGGTGGCTCACGGCCCCGTGTGCAAAATCAGCCCCGAGGAACGCCGATCG CCCCCCACCTCCGGGCTGCAGTCCTTGCGCGGTACGGGCAGTGCCGTTGGCCCCGTGGTGGTGAAGCAGG AGCCTCCCGAAGAGGACAGCCCCTCTTCCTCAGCAGGGATGGACAAGACCCAGCGCTCTGTCATCAAGCC CATCAGCATCGCCGGGGGTGGTTTCTACGGGGAAGAGCCTCTGCACACCCCCATCGTGGTGACCTCCACG CCTGCCATCACTCCCGGCACTTCAAACCTTGTCTTCACCTACCCCAATGTCCTGGAGCAGGAGTCGCCTT CGTCGCCCTCAGAGTCCTGCTCCAAGGCTCACCGCAGAAGCAGTAGCAGTGGGGACCAGTCATCAGACTC CTTGAACTCCCCCACACTTCTAGCCCTGTAACCCTGTGCTCCTCTGGCCAGCTCTGGAGGAATCCTCATC CATCATTACCACACCCTTCCCAGGGACCAGCACCTTTAAGCACTCCAGGGCCTTGAGGGAATAAGGGGCC CCTGGCTCTGGGGACCCACCCGGTGGGACTTAGTGGTGAGACACTGGTTGATCTCTTAGAAGCCCTGGGA CACGCCCTCCCTCATTCATCTTGCAAGCGAAGCGAATCCTTTTTCTTGAGAAGCCTCAGAGAACTTGGTT TGTGGACTCAGACGCGCCTGCGGCTACCCCCACCTCACCCCACCCCCCCCTCTTCTGAAGCCCCGCCCCC TGGAGCTGGCCCGGATGGTTTCTGTCCCTTTGTTATCCGTTGTCTCTGAAGTGGACAGTATCCTTTCCTG CCCCGCCAAGCATGCTCAGTGCCTTTTGGTTTCCCCTCCTTCCCCTAGATTCAGTTTTGCTCCCTCTGGG TTTTTATAAGATTTGCCATGACATTTCATCTGGGTGGTCCAGATAGTAAAGCGCTTTTATTTCTGGAGCT GGGGAAGCAGATGACTCTTCCACTGGGGCGGAAGGGGCACCCACTGTGTCCCGAAGGGGCAGTCAAAGTG CAATATTATTGAAACTTCCCTCCCTACACACCCAGTGCGCTGTGGTTACAGACCGTGGGCTCCCTGAGTT CTGCCGGTGAGCTGCCGTCACTGGGATGCGCCAAGGACCTTTCCTCCCCGGTCTCAGAGATGCACATCTC TTTGAGGATCGGCTTGGCTGGAAGACAGGGTGTGAGTGCGACAACAGGGGCACAGGTTGGGTTTGCCAAA CGTCTAATTACCAAGCCAGGATTTGTGCCAACAAAGCCACATGGCTGTCCTAGTGAGCTTCCTTTCTTAC CTGTCTTGAGCTGGGCGTCTCTTTAATTACTGCCTCTGCCCCGGACCCTTCTCTCTAGACTTGGGAAGCA CTCCTTCCTAGAAGCCACACATCACTCCAAGGCTCAGCTGGGCGCTGCTCATCCCCAACTCTCATAGTGA CATCACCTGTGGATATTTAATGAGTGTGGGATGCGGGGTCTGCGGTAGAGATGGTTTCTGGCAGCCGCAG CAGCCCCAGGTACCTAGCCTCCCCTGGCTGCTCTCCTGTCAGTGAGATGCAGGAGCCGCCCTCTGCTCTC AGTGGTCTCTGCTACTGATGAATCCAGTGGGTTTCCTTTCATATTCTCCTGGCCAACTCCAGAGTGGCCT CCTAACCCAGCAGGCCCACCCCTCTGGGAGTCGTTGGTCTCCACCAGTTTTGTTAGGCTTTGAGTGTCTA TTCGGTTCCTATCAAAGCTAGTGTGTATTCCTTTCCACTACAGAAGAACCATCACTCTCATGGGTGTCCC AGCCTCCAGCAAGGGCACAGCTCTGTCCCCTGCCTTTCTCTCCCTTTGTCCCCACTGCATGTTGACACAA GTATGGAAGTGGAGCCTGGTTAGAACTTGTTTCTAGTGCTAATGCTCTGACCTAGTATTTCCATTGGAGA TCACTGTCCAGCCATCTAAAGCCCTGTTATTCGAGTCAAGCTTATCCCCATTTTTGAGAGAGAAGGGGTA CCTTCACAGACCGATGCCCTTCTTACCCCAAGTTCCCATAAACACTTGCATGCAGAAGCACACAGGACAC AGGGACCATGTGGGAAGCATGCTCAGCCTAGCAGAGCTGAAAGGAGCAGGCTTGGCTTGGTAGAGAGGGG GGCAGAAAGGAGATAATCTCAATGTGTTCTTTCCTGTATGCCTTGGCTCTAACTCTGCTGCTCTGGTCTC CCAGAGCCTCTAGGGGCAGGGGAGTGCTCCACAAGGTGGTTTGCTGGCTTCAGAGCCCTAGAAGCACAAG ACAGTGGAGCCAGCGGAGCCGGGACTAGAGGAGTTGGGCAGTCTCAGTTGAGAAGCTTTATCGCCTCAAG CCAAGTGGGAGCTCTCTCCCTCATGCCTGTGGGTATTTCTGAAATGAAGCTGCGCCCTGTTCTGTCCTTT CCCAAAGCCCCCTTCTGCCCATAGCATGGATCCAACCCAGGTCACAGGCCTGGGACTTGGTACCCTGGTT GGACACTCCTGAGACTCCAAGTCTGGCTTTCAGTTCAGTTTTCATCTGTGTTGACATCGGGGCCAGAAAG CACAGCCAGCTAAATGGGCCTCTGAGGTCACCTTCCCTTCCGGCCTGCTTGCTTTGTCTTACAAGACTTC TTGGCTTTTCCAAAGACAGAGATTTCTGGGGGGAAGCCGAATATTTCTACCTAAACTCTGGGTGTGACCT CAGCTCGCTTTCCTCTGATCCACTGTCCAAGTTGGGTCACAAACATGTCACTCATGCATGCCTGCACTCC AGAGTCAGGTGCCAAGCTTGCTTGTCCTTCCCTGCCTCCAGGTCAGGGAGCAGCTGCAGTGAGGGTGGGG AGGTGAGGGCTTTGTACACGTGTGTCCTCTGCTGTCATGAACTTGGGCTGGACTGTGAGCAGCTCTGGGT TAATACAGGGCAGGCAATCCTTAAACAGCTAGCATGAAACAGTTTTGTCGTGGCCACCAGGCTTCTGTGG GAACAGTCCTTCTCTGGCTGGCTAGCTAGGCACAGCTACGGCACTGGAAAGAGTAGAGCCCAGGTGAAGC TTAGGAAATTGGGAGCTAACACCAAGCAACTGGTTCCGTGGGGGAAGGTTTTAGGGAGCTCAGACTGCTG CTTTCATAGGCAAAGTGTGCCCATGGGGGCCAGTCATGGGAACATGTCAAAGCCAACAGGAAGCAGAACC TGTAGCTTTCTCTGGCATTAGGGAGAGAGAACCCTGCTTGCTTCCACTTAACTCCTGGTGGAAGAGGGGC AGGCACGTTCGAAGCTTCTGGATTCATTTTTCCTCCACTTTCCTGAGAGACCAAAAGTGTTCCTTGAGAT GTCACCATTTGTCTTTCACAGTGACTTCCTTCCCTGAGCAAACATTCATGTGGCTTCGCTGGGGAGGAAA AAAATAAGCACCTACTGTGTGCCAGGCACTATGAGGCACTTTTCTATTTATCCTCTTTTAAGATAGGACT AAGAGTTCATCTCCTTCTAGAAGGCCCCCAGGTTAAATAAAAACACTTGGGGGTAAGAGGGCTTTCTTCA GAGACTCCCAGGTGGGAGTGCCTCTAGTCTGGTGGTCCTCAAAGACTCATCCACAGCAGCATCTGCCCAT CCCAGGCTCCTCTCTCCTCAGACTCCTCCTATCTGGCTTGTGCTCCTCAGGTGGCTGAAGGGGCCACCTT AGTTCTCATAGAAATTGTGGACCAATAGGATATGTGCTGTGTATAACTTTCTTTTTAAACTGTAAGTAAA GGGTCTTGCAGCTTTGTGCTTGTGTTATCTTAGCATTTATCTTCAAACCAGCATCTCACTATTTATTGAC AGTGGTGTGTGTGTGTGTGTGTGTGTGTTTCTGCGTGTGCACATATGTGTGCACACATGTGCGTGTGTGT GTTCATGTGTGTGCATGCTTGTGTCTTTCCACAGCTTTTTTTTTTTTTTTTAAGGATCATCCTACTGCTA AGTCCGTGTCTCTTCTTGCTTCTAGTGTTTTGGCCACGCCTCACCAAATGTCTGTAATGACCCAGTACTC ACAACATGTTCAGGAGGAGCTGGGTCAGATTTTGACAGAGGGTATGGGAAGGGAAAGGGGAGAAGAAATT GACATTTATTTTATTATTTATTTTAAATGTTTACATTTCTTTGTGTTGTTCCAAGCCTGAATAGAAACAG ATAGCATTAAAGTACTTCCTTCCCACTCCTTTCTCTGGCTCTCTCTCCCCCACTTGTTCTGACTTAGGAT AATGATCTCTATTTTGTTTGTTTCTCAAGTGACTTGTGGACTTGTGCTGTATAAACTGTATTAAAAAGGC TCTGTTTTTAAAGATCAATTGTCATTCCTGTGGGGACAGTGGCTACTGAGAAATCTACATTGTAAGAGAA GACAATGAAAGACCCTGGCCCTGTCTCTCAAAACTTAACTTTCTCTGTATGATTTTTTTTTTAAAAAAAT ATTCCATTTATTTTACTTTGTGGTTACTTGATTTTGAGGAAGAAAATATTCAGCTTTGTATAAAGACTAG GTATCAGATTCCTTTTGCAGTGGGAGGTGTATATATCGTATTTTGGTATAGAGTAGGACCCAAGCTTCAT GCATCTGTATTTGGTATATGTCAATGACGTGGAGTGAAATTTGCTATTAGATCCTGGAGGCAAATGGGTT GTACAAGGTTTTATGGCTCCGCGGGGAATTTAATTTCCTTTCTGGGCACCTTTTGTCCCCTACTTTTAAA TTAACGGTGAAACGGCCCTAGGAGGGTCGCTCTAGTTGAACTCTCCAGGCAGGACCTTATGCTCAGAAAT CTTTGTATAGTTTTAAATTTTTGAGGAGTATCTCTGCTCGGAAGCATCTGTGGTGGTGTGATGTGTTGTT CTGTGTACTGTATGTGACACAAGCCTACAGGATTTGCACTAAGAAAAGCTTGGTAGGAGCTTGCTGCTAT GGAGGAAAGAACATATTAAAAACTTATTTTCCCTCGGGTTTGTTCTCGTTTTATGTTTTTGTTGTTGTTG TTTTGTTAGCTTTCCTACTTCCACTGAGTAGCATTTTGTAGAATAAAATTAATCAAGAACAAAAAAAAAA AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30536144 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30536144 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18262 same embryo
	EMAGE:18263 same embryo
	EMAGE:18259 same embryo
	EMAGE:18260 same embryo
	EMAGE:18261 same embryo
	EurExpress:euxassay_012044 same experiment
	MGI:4824896 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source