EMAGE mouse gene expression spatial database

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EMAGE:18264

Zfp187 zinc finger protein 187 ( MGI:3531417)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012055. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012055_01	euxassay_012055_02	euxassay_012055_03	euxassay_012055_04

euxassay_012055_05	euxassay_012055_06	euxassay_012055_07	euxassay_012055_08	euxassay_012055_09

euxassay_012055_10	euxassay_012055_11	euxassay_012055_12	euxassay_012055_13	euxassay_012055_14

euxassay_012055_15	euxassay_012055_16	euxassay_012055_17	euxassay_012055_18	euxassay_012055_19

euxassay_012055_20	euxassay_012055_21	euxassay_012055_22	euxassay_012055_23	euxassay_012055_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18264_wholemount_strong.wlz

18264_wholemount_moderate.wlz

18264_wholemount_weak.wlz

18264_wholemount_possible.wlz

18264_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18264_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 06 07 08 10 11 12 16 17 18 19 20 moderate expression: see section 01 02 03 04 05 09 21 22 23 24

hindlimb digit 1 metatarsal

moderate

regional

moderate expression: see section 05 06 23 24

hindlimb digit 2 metatarsal

moderate

regional

moderate expression: see section 05 06 23 24

hindlimb digit 3 metatarsal

moderate

regional

moderate expression: see section 06 23 24

hindlimb digit 4 metatarsal

moderate

regional

moderate expression: see section 06 24

hindlimb digit 5 metatarsal

moderate

regional

moderate expression: see section 06 24

vibrissa

weak

regional

weak expression: see section 04 05 06 22 23

cerebral cortex marginal layer

strong

regional

strong expression: see section 03 04 05 06 07 08 19 20 21 22 23 24 moderate expression: see section 09 10 11 16 17 18

olfactory cortex marginal layer

strong

regional

strong expression: see section 11 12 13 15 16 moderate expression: see section 10 17 18

telencephalon ventricular layer

strong

regional

strong expression: see section 04 05 06 07 08 19 20 21 22 23 24 moderate expression: see section 09 10 11 16 17 18

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 07 09 10 11 18 19 20 weak expression: see section 08

outer ear

moderate

regional

moderate expression: see section 03 04 05 06 22 23 24

inner ear

moderate

regional

moderate expression: see section 07 08 09 20 21

otic capsule

moderate

regional

moderate expression: see section 10 11 17 18 19

neural retina

weak

regional

weak expression: see section 03 04 23 24

esophagus

moderate

regional

moderate expression: see section 13 14

stomach

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12

hindgut

weak

regional

weak expression: see section 14 15 16

midgut

moderate

regional

moderate expression: see section 13 14 17 18 19 weak expression: see section 15 16

metanephros

moderate

regional

moderate expression: see section 10 11 17 18 weak expression: see section 09

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9548
Entity Detected:	Zfp187, zinc finger protein 187 ( MGI:3531417)
Sequence:	sense strand is shown >T9548 TGGTTTTTTTTTGTAAACCGGTTTCCTAATTATCTGTACTTATCTCTAGGACTCAGCCCAAAGAAGCAGA CCAACGGTGCTGGTTGGCGAGCCAGCCCCTCGCAGGGAAGCACGGGAGCAGCCTGGGTGTGCGCTTCCTC AGAAGCCTGAGGAGAGAGGTAAAGAGACAAGGAGTGAGAATGGGAATCTCATTGCAGGGACAGACTCTTG TGGAAGAATGGAATCATCTTGTACCATGACTGAACCCATTGAGGCCCAGTGTGAGGACCTGAGCTTGAAA AAGAATCCAGCCATGCCCAAGGAAAAAAACTAACAGCCAATGCTTGGAAACTAAGGAGAGACTTGTTCAG AACTCAGGCCTGATTGAACATGACAGGGCACATACAGGAGAAATGTCCTGGGAATCTGTAGGATCTCAGA GTTCTGTTGCTGCTGACCATCAGGAAATCTCTAAAGACAAAGGTCATCCCTGCCAAGAGTGTGGGAAAGT CTTTCAGAGAAGTTCACACCTCATCAGACATCAGAAAATCCATCTTGGGGAGAAGCCTTACCAGTGCAAA GAGTGTGGAAAAGTCTTTAGCCAGAATGCAGGTCTTTTGGAGCATCTCAGGATCCATACTGGAGAAAAAC CCTATCTGTGTATCCATTGTGGAAAGAACTTCAGGCGAAGCTCTCATCTTAATCGACACCAAAAAATTCA CAGTCAGGATGAGCCCCGAGAATGCAAGGAGTGTGGGAAAACCTTTAGTCGGGCCCTCCTCCTCACCCAC CATCAGAGAGTCCATGGTCGTTCCAAAAGACATCACTGTAATGAGTGTGGGAAAGCCTTCAGCTTGACCT CCGACCTTATTCGACATCATAGGATTCACACTGGAGAAAAACCTTTCAAATGTAATGTGTGTCAGAAAGC TTTCCGCCTAAATTCACACTTAGATCAGCATGTTAGAATCCACAATGAAGAAAAACCCTACAAGTGCAGT GAATGCAATGAAGCCTTCAGGCAGAAGTCTGGTCTCTTTCAGCATCAGAGACATCACCATAAAAGCAAAC TGGCTTGATGAGGTTCTCTGTTTGAAACATGGAAATGTTACATTGATAAGCAAACAGCGCCTTTTGAAAG AACTCACGACGGCTTCAGGATTCTTGGAAGCCATTTGCCTCCGTTGTGTCCCTCTCTTGGCTTTACTTAA AGTCATAATTAGACTATAATAATTTACCAGAATAGCAGAATCAGAGTTAAACAAATATTCACTACAGAGC TTCTCAGAGCTTTCAGTGTGATAAGTACTTGATTATGTATCTCTTAAGTATTAGCTTTATGTGTGAATGA AGTGTTTGAAATCAGTAAGTCAAGTGCCTATAGATTTATACAGGACAAGATCAAGCTGATATTTTTGTAA GTACTGTAGCAACAGGCTCCTAAAACATAAAATTAATGAGGGTTGGGGATATGCTCAGTGGTTTAACCAA GTTGCTATGCAAGTGGAAGGACTGTTTTGGATCCCCAGCACCCATGTAAAGTCAAATGAGTATGTGGCCA GCCTGTGGCCTGTGGTCCCACCCCTCAGGTGGCAAAAATGGGATCTTTCTAGCAAGCTGGCTAGCCAGAC TAGCTGTATCAGCAAACTTTGGACTCAGGGGAGGGAGTTTGGAGAGCAGTCGGTGGAGTTCTTGAGGAAG ACTCCTGATGATAATCTAAGTGCACATGTGTCCATACGTATGTAGAGCCATACCTGCAAACATCATACAT GTGAAAAGACAAATCCTAAGGAGCTAGAGTTAGCAGTTAACATACCTGCTCTTGCAGAGGACCCATCCAT TTTCAGTTCCTAGCACCTCCATAGCACAGCGTCTCACAGTTCCAGTTCTGGGGATCTGCTAGCCTTCTCA GGCTTCTGTACACAGTGGTCCACAGAAACTGGTGGACACACACACACAACTGGTAAAATATTTTTAAGCC CAAAATAAATCTAATAATGCTTAGAAATACACATCCTCAAGGGTCTAACGTAGGTTAACTGAACATTTTC CCTGTTAGGTTCACACAAACACTGGCTTCATATCCACTTTACCTTTGTACCATCAATGAATGCATTTCTG AAAGCTTTTGCTGTTTAACTATAGAACCTACCCTTAGGATTAGCTCATGTCATTTTACATTTCCTGCCCA TTTTAACCACAGTATCACAGTGTTTCAAATTATAAATCAAAGTTTTACCTAAACTATGCAAGAGACTTTG AGTCATTTGAGTGAACTGTGTTGAAGTATTCTAAGCAGCGACCTCCTTACTGGGCAGCAACAGTGCCAGT ACAGTGACTGCTTGAAGGACACCTTTTTTCATGAATGTCCCATGGGGGGAAATGGGGTTTTCCCCCTTTG ATACCAATGATGCAAATTTAAATGCTACTTGTTTTGATATTATTTTGACCATGCAAATCAAATCAGCTCG GAGAGATCATGGCCATCAGGTCATATTGACTGTCTTGCTAGTGATGAAAAGTAATAAGCTTGGAGTCTTC CCAAGCCCAGCACCCGACTGCTAAACATGGCTTATGGTTGGTAATTGCACAGGGTGTACTTCTCAGGGAA AGGGCTTCTTAAGTTATGCCGCTTTGGGAAGAGAAGGGGACCCTTGTACTGACTACAACTTGGTTTCACT ACACTTGACTTACAAATAAGCCAAGAACTTAGCAACATAGAAATGCTTATTTTTCTTGTCCGATAGAAAA GGATAGTCAGTCCAAAACAGATCCATGGTAAACCTAGTCAGCTTGCTGCCAGTCATGTCTAAGGAGGGGA CAGGCCAAGGACTGCTAGGCACCTAGGTGCAATTCTGACATTAATCTTCTGTTTCATGCCCTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTGGTTTTTCGAGACAAGATTTCTCTGTGTAGTCCTGGCTGTCCTGGAACT CACTTTGTAGACCAGGCTGGCCTCGAACTCAGAAATCCGCCTGCCTCTGCCTCCCGAGTGCTGGGATTAC AGGCGTGTGCCACCACTCCTGGCTCGTTTCATCCCCTTTTAACCAAACTTTGCTCTGCACAAAATAAGGC CCTCTTCCTTTGTTAAGATTTGACCTAAGATACACTCTAAGCAAAATTGGGGAGAGCTGCACAAAGGGAC TGGCTGAACTGAGAAGAACCTCTCTTGGACCTTTTGCCCACACTTTGCATGTGATAGTTGCAACTTCAGC AGTCATATGGCCTTAAAAACTGATGGCATGGACCAAGGTTAGGTCCTCAACACCACCCAAAATAACAGTG TTAGCGTGTGATCAGTCTCCTTCATGTTTAACTCCGATCCACACCAAACGTGCATGTAACAGGCATTGTA ACCCTTGTAATTCCTAAGCCTCAGAAGAAACACAGTCTTAGAACATGCTTTACATCTCTACTGTGGAACA TCCTTTTATGCATCACTGTTTGTGTGAAAGTGATGTTCCTTTTCTGGCCTTTCTCTTAGCTTCTCCTTTT ACTTCCAAACAGCATGGCCCTTAAAAATTAGAGAACTATCAAAATTTTAAATGCACATTATTCTTCGTCA GTTTGTGTGTGTAATATTTACTCAACAAATATATTTAAAGCAACATAAAAACATAATTATTGATAGTGTA AAGAGAATACTTTTCTATGGAAATAATGATATTGACAGTATTGTAAACAGTCTGTAAAGTGTTAATAAAG TTTCAAAGTAATTTTTGAATAAAATTAAAGCTATAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA A
Notes:	The probe template was PCR amplified from IMAGE:30547278 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30547278 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18266 same embryo
	EMAGE:18265 same embryo
	EMAGE:18267 same embryo
	EurExpress:euxassay_012055 same experiment
	MGI:4829295 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source