EMAGE mouse gene expression spatial database

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EMAGE:18296

Frmd8 FERM domain containing 8 ( MGI:1914707)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012085. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012085_01	euxassay_012085_02	euxassay_012085_03	euxassay_012085_04

euxassay_012085_05	euxassay_012085_06	euxassay_012085_07	euxassay_012085_08	euxassay_012085_09

euxassay_012085_10	euxassay_012085_11	euxassay_012085_12	euxassay_012085_13	euxassay_012085_14

euxassay_012085_15	euxassay_012085_16	euxassay_012085_17	euxassay_012085_18	euxassay_012085_19

euxassay_012085_20	euxassay_012085_21	euxassay_012085_22	euxassay_012085_23	euxassay_012085_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18296_wholemount_strong.wlz

18296_wholemount_moderate.wlz

18296_wholemount_weak.wlz

18296_wholemount_possible.wlz

18296_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18296_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 05 06 18 weak expression: see section 03 04 07 08 09 10 11 16 17 19 20 21 22 23

humerus

strong

regional

strong expression: see section 01 moderate expression: see section 02 03 04 05 22 23 24

fibula

moderate

regional

moderate expression: see section 01

tibia

moderate

regional

moderate expression: see section 01 02

femur

strong

regional

strong expression: see section 05 06 moderate expression: see section 02 03 07 19 20 21 22 weak expression: see section 04 18

otic capsule

weak

regional

weak expression: see section 09 17 18

axial skeleton

moderate

regional

moderate expression: see section 18 weak expression: see section 09 10 11 12 13 14 15 16 17 19

basioccipital bone

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16

basisphenoid bone

weak

regional

weak expression: see section 11 12 13 14

exoccipital bone

strong

regional

strong expression: see section 01 02 moderate expression: see section 05 06 19 20 21 22 weak expression: see section 03 04 07 24

temporal bone

strong

regional

strong expression: see section 01 02 moderate expression: see section 05 20 22 weak expression: see section 03 04 23 24

orbito-sphenoid

strong

regional

strong expression: see section 01 02 moderate expression: see section 05 06 18 19 21 22 weak expression: see section 24

viscerocranium

moderate

regional

Expression in the turbinate bone.

clavicle

moderate

regional

moderate expression: see section 06 weak expression: see section 08 17 20 21

scapula

strong

regional

strong expression: see section 05 moderate expression: see section 22 weak expression: see section 04 23 24

pelvic girdle skeleton

weak

regional

weak expression: see section 09 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T372
Entity Detected:	Frmd8, FERM domain containing 8 ( MGI:1914707)
Sequence:	sense strand is shown >T372 GCGGGGCCTCGGGTTTGTGCTTCCTCCCGCGGCAGCTTCTGCGGCGTAACGCCTGCGAATCGACCCTGGG CAGGACCGGAGACCCCGCCGGCCGGTGTGGTGGTGGGTCCAGAACTGGAGGCCTGAGTCGGGTGGCATTC TGGAGTGAGGCAGTGGGCTCTGCGACCCTGCAAGATGGAGGGGGCAGAAGGCAACGCGGGGCAGCCCGGC CCTGCGGAGCGGTCCCACCGAAGTAGCGTCTCTTCAGTGGGTGCCCGAGCAGCCGATGTCCTGGTGTACC TGGCGGATGATACAGTAGTGCCCCTGGCCGTGGAGAATTTGTCCTCAATCAGTGCCCATGAGCTGCACCG CGCAGTCCGTGAAGTCCTGCAGCTCCCAGACGTGGCTTTGGAGGCCTTTGCACTCTGGCTCGTCTCCCCT CTCTTGGAGGTGCAATTGAAACCCAAGCACCAGCCCTATAAACTGGG
Notes:	The probe template was PCR amplified from IMAGE:3156148 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3156148 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18298 same embryo
	EMAGE:18297 same embryo
	EMAGE:18294 same embryo
	EMAGE:18293 same embryo
	EMAGE:18295 same embryo
	EurExpress:euxassay_012085 same experiment
	MGI:4824927 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source