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EMAGE:18352

Pop5 processing of precursor 5, ribonuclease P/MRP family (S. cerevisiae) ( MGI:2151221)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18352 EMAGE:18352 EMAGE:18352 EMAGE:18352 EMAGE:18352
euxassay_014299_01 euxassay_014299_02 euxassay_014299_03 euxassay_014299_04 euxassay_014299_05
EMAGE:18352 EMAGE:18352 EMAGE:18352 EMAGE:18352 EMAGE:18352
euxassay_014299_06 euxassay_014299_07 euxassay_014299_08 euxassay_014299_09 euxassay_014299_10
EMAGE:18352 EMAGE:18352 EMAGE:18352 EMAGE:18352 EMAGE:18352
euxassay_014299_11 euxassay_014299_12 euxassay_014299_13 euxassay_014299_14 euxassay_014299_15
EMAGE:18352 EMAGE:18352 EMAGE:18352 EMAGE:18352 EMAGE:18352
euxassay_014299_16 euxassay_014299_17 euxassay_014299_18 euxassay_014299_19 euxassay_014299_20
EMAGE:18352 EMAGE:18352 EMAGE:18352 EMAGE:18352
euxassay_014299_21 euxassay_014299_22 euxassay_014299_23 euxassay_014299_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18352Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18352_wholemount_strong.wlz
18352_wholemount_moderate.wlz
18352_wholemount_weak.wlz
18352_wholemount_possible.wlz
18352_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18352_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 01 15 16 17 18 moderate expression: see section 02 03 04 05 06 07 08 09 10 11 19 20 21 22 23 24
humerus
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 21 22 23 24
fibula
moderate moderate
regionalmoderate expression: see section 24
tibia
moderate moderate
regionalmoderate expression: see section 23 24 weak expression: see section 01
femur
moderate moderate
regionalmoderate expression: see section 04 05 06 17 18 19 20 21 22 23 weak expression: see section 01 02 03
meckel's cartilage
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 11 14 15 16 17 18 19 20 21 22 moderate expression: see section 09
axial skeleton
strong strong
regionalstrong expression: see section 13 14 15 16 17 moderate expression: see section 08 09 10 11 12
basioccipital bone
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18
basisphenoid bone
moderate moderate
regionalmoderate expression: see section 07 08 09 17 18
exoccipital bone
moderate moderate
regionalmoderate expression: see section 06 19 weak expression: see section 20
temporal bone petrous part
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 weak expression: see section 20 21 22 23 24
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 02 05 06 07 08 11 12 13 14 15 17 18 19 weak expression: see section 23 24
viscerocranium
moderate moderate
regionalExpression in the turbinate bone.
clavicle
moderate moderate
regionalmoderate expression: see section 08 09 16 17 19 20
scapula
moderate moderate
regionalmoderate expression: see section 02 03 04 22 23 weak expression: see section 21
pelvic girdle skeleton
moderate moderate
regionalmoderate expression: see section 07 08 15 weak expression: see section 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31469
Entity Detected:Pop5, processing of precursor 5, ribonuclease P/MRP family (S. cerevisiae) ( MGI:2151221)
Sequence:sense strand is shown

>T31469
ACAGCAGGTCCCAGATCGTGCGACACTCTCGGACTTTAAGTGCAGGGCTGACCTTAAACGTTCACTTGGG
GGACTCTGAGCGCACAGAACTTGTGTGGAAAATCCCCAGCGCCTGCCGGAGGCGAACGCATGCGCTCTTA
CGCCCGCCGCGGGAGGCGGAGCGTGGAGATTGACAGGGCACGCTGCCTGTCCGAGCCTAGTGCCGCCTTC
CTCCCGCATCCGGCGCGGACGCCATGGTGCGGTTCAAGCACAGGTACCTTCTGTGCGAGCTGGTGTCCGA
GGACGCGCGCTGCCGTTTGAGCCTGGACGACCGCGTGCTGGGCGGGCTGGTCCGCGACACGATCGCCCGG
GTGCACGGGGCCTTCGGCGCCGCCGCCTGCTCCGTGGGCTTTGCAGTCCGATACCTCAATGCCTACACTG
GAGTCGTGCTACTGCGATGCCGGAAGGATTTCTACCAGCTCGTGTGGTCAGCTCTTCCTTTCATCACCTA
TTTGGAGAACAAAGGCCACCGTTACCCGTGCTTCTTCAACACATTACACGTGGGAGGTACCATCAGAACC
TGTCAGAAGTTCCTGATCCAGTACAACAGGAGACAGCTGCTGATCTTGTTGCAGAATTGCACTGACGAAG
GAGAGCGGGAAGCCATCAAGAAGTCTGTCTCAAGAAGCTGTCTGCTGGACAGAGAGCCCGTTGAAGAGCT
TTCAGACAGTGCTGGTGAGGAGGTTGCAGAAGCAATGGAGTGACCGTTTCCAGGCCCACTTGTGGGAACA
GGACATTACAGGAGGTGACAACAGCATCCCACAGTTCTCCAATGGAAATGACTGATAACCAGCACTCAGG
TGACCAGCTTGAAGTGGAGGGCTTGCTGCCTTACCTTGCATCCATGGTTGTGAAGGCTCTGTGCAGACAT
TTCGGATGTAACTGCTTAGCTCAGGGGTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4224973), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 26548. Forward Primer - name:026548_F_IRAV59-62_N03_Pop5, sequence:ACAGCAGGTCCCAGATCG; Reverse Primer - name:026548_R_SP6_IRAV59-62_N03_Pop5, sequence:CCACCCCTGAGCTAAGCA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18354 same embryo
 EMAGE:18356 same embryo
 EMAGE:18353 same embryo
 EMAGE:18355 same embryo
 EurExpress:euxassay_014299 same experiment
 MGI:4827333 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS