EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:18353

Cul7 cullin 7 ( MGI:1913765)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014294. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014294_01	euxassay_014294_02	euxassay_014294_03	euxassay_014294_04

euxassay_014294_05	euxassay_014294_06	euxassay_014294_07	euxassay_014294_08	euxassay_014294_09

euxassay_014294_10	euxassay_014294_11	euxassay_014294_12	euxassay_014294_13	euxassay_014294_14

euxassay_014294_15	euxassay_014294_16	euxassay_014294_17	euxassay_014294_18	euxassay_014294_19

euxassay_014294_20	euxassay_014294_21	euxassay_014294_22	euxassay_014294_23	euxassay_014294_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18353_wholemount_strong.wlz

18353_wholemount_moderate.wlz

18353_wholemount_weak.wlz

18353_wholemount_possible.wlz

18353_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18353_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08

humerus

strong

regional

strong expression: see section 01 02 03 04

fibula

strong

regional

strong expression: see section 02

tibia

strong

regional

strong expression: see section 01 02

femur

strong

regional

strong expression: see section 01 02 03 04 05 moderate expression: see section 06

meckel's cartilage

strong

regional

strong expression: see section 03 04 05 06 07 moderate expression: see section 08

axial skeleton

strong

regional

strong expression: see section 08

exoccipital bone

strong

regional

strong expression: see section 05 06 07 moderate expression: see section 02 03 04

temporal bone petrous part

strong

regional

strong expression: see section 01 05 06 07 moderate expression: see section 02 03 04 08

orbito-sphenoid

strong

regional

strong expression: see section 01 02 05 06 07 moderate expression: see section 03

viscerocranium

strong

regional

Expression in the turbinate bone.

clavicle

moderate

regional

moderate expression: see section 05 06 09 16 weak expression: see section 08

scapula

strong

regional

strong expression: see section 02 03 04

pelvic girdle skeleton

moderate

regional

moderate expression: see section 07 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31471
Entity Detected:	Cul7, cullin 7 ( MGI:1913765)
Sequence:	sense strand is shown >T31471 CTCCGTCTGCCAAATGCTCAACCCGGCAACGTGCCTGCCCGCATACCTGCGGGGGACCATAAACCACTAC ACCAACTTTTACAGCAAGAGTCAGAGCCGCTCCAGCTTAGAGAAAGAGCCACAGAGGCGGCTGCAGTGGA CCTGGCAGGGCCGGGCAGAAGTGCAGTTCGGGGGTCAGATTCTGCATGTGTCCACAGTACAGATGTGGCT GCTGCTGCATCTCAACAACCAAAAGGAGGTGTCTGTCGAGAGCCTGCAGGCTATCTCGGAGCTCCCTCCA GATGTGCTTCACAGGGCCATCGGGCCTCTCACCTCATCAAGAGGTCCCTTGGACCTGCAGGAGCAGAAGA ACGTACCAGGAGGGGTGCTCAAGATTCGAGATGACAGTGAGGAACCCAGGCCGAGGAGGGGCAACGTGTG GCTGATCCCACCTCAGACATACCTACAAGCTGAGGCCGAAGAGGGCCGGAACATGGAGAAGAGAAGGAAT CTTCTGAATTGCCTTGTTGTCCGAATCCTCAAGGCTCACGGGGATGAAGGCTTGCATGTTGACCGGCTCG TCTATCTGGTGCTAGAAGCGTGGGAGAAAGGCCCGTGTCCTGCTAGGGGTCTGGTCAGCAGCCTCGGCAG GGGAGCAACCTGCAGGAGCTCTGATGTCCTCTCCTGCATCCTGCACCTCCTGGTCAAGGGCACGCTGAGA CGCCATGACGACCGGCCGCAGGTGCTGTACTATGCAGTTCCTGTAACTGTGATGGAGCCCCACATGGAGT CCCTGAACCCTGGCTCGGCAGGCCCCAATCCACCCCTCACCTTCCACACCCTGCAGATTCGATCCCGGGG TGTGCCTTACGCCTCCTGCACTGATAACCACACCTTCTCTACTTTCCGGTAGCCCTGGATATGGGGTTGT GG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4022086), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 26101. Forward Primer - name:026101_F_IRAV59-62_O05_Cul7, sequence:CTCCGTCTGCCAAATGCT; Reverse Primer - name:026101_R_SP6_IRAV59-62_O05_Cul7, sequence:CCCACAACCCCATATCCA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18354 same embryo
	EMAGE:18356 same embryo
	EMAGE:18355 same embryo
	EMAGE:18352 same embryo
	EurExpress:euxassay_014294 same experiment
	MGI:4824125 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source