EMAGE mouse gene expression spatial database

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EMAGE:18411

Caly calcyon neuron-specific vesicular protein ( MGI:1915816)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014371. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014371_01	euxassay_014371_02	euxassay_014371_03	euxassay_014371_04

euxassay_014371_05	euxassay_014371_06	euxassay_014371_07	euxassay_014371_08	euxassay_014371_09

euxassay_014371_10	euxassay_014371_11	euxassay_014371_12	euxassay_014371_13	euxassay_014371_14

euxassay_014371_15	euxassay_014371_16	euxassay_014371_17	euxassay_014371_18	euxassay_014371_19

euxassay_014371_20	euxassay_014371_21	euxassay_014371_22	euxassay_014371_23	euxassay_014371_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18411_wholemount_strong.wlz

18411_wholemount_moderate.wlz

18411_wholemount_weak.wlz

18411_wholemount_possible.wlz

18411_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18411_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 09 10 13 14 moderate expression: see section 01 02 03 04 05 06 07 08 11 15 16

radius

strong

regional

strong expression: see section 01 moderate expression: see section 24

ulna

moderate

regional

moderate expression: see section 24

humerus

strong

regional

strong expression: see section 01 02 03 04 moderate expression: see section 20 21 22 23 24

forelimb digit 3 metacarpal

strong

regional

strong expression: see section 02 03

forelimb digit 3 phalanx

strong

regional

strong expression: see section 02 03

hindlimb digit 2 metatarsal

strong

regional

strong expression: see section 06 07

hindlimb digit 2 phalanx

strong

regional

strong expression: see section 06 11 moderate expression: see section 12

hindlimb digit 3 metatarsal

strong

regional

strong expression: see section 06 07

hindlimb digit 3 phalanx

strong

regional

strong expression: see section 06 11 moderate expression: see section 12

hindlimb digit 4 phalanx

strong

regional

strong expression: see section 11 moderate expression: see section 12

hindlimb digit 5 phalanx

strong

regional

strong expression: see section 11

fibula

strong

regional

strong expression: see section 01 02 03 04

tibia

strong

regional

strong expression: see section 01 02 03 04 05

femur

strong

regional

strong expression: see section 01 02 03 04 05 06 07 17 moderate expression: see section 18 19 20 21 22

brain

strong

regional

strong expression: see section 05 06 09 13 14 15 18 19 20 21 22 23 24 moderate expression: see section 01 02 03 10 11 12 17 weak expression: see section 04 07 08 16

spinal cord

strong

regional

strong expression: see section 09 13 14 15 moderate expression: see section 10 11 12

otic capsule

strong

regional

strong expression: see section 06 07 08 09 15 16

nasal septum

strong

regional

strong expression: see section 12

viscerocranium

strong

regional

Expression in the turbinate bone.

meckel's cartilage

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16

axial skeleton

strong

regional

strong expression: see section 09 10 12 13 14 moderate expression: see section 06 07 08 11 15 16

basioccipital bone

strong

regional

strong expression: see section 01 02 03 05 06 07 09 10 13 14 21 22 moderate expression: see section 04 08 11 12 15 16

basisphenoid bone

strong

regional

strong expression: see section 09 10 13 14 21 22 moderate expression: see section 11 12 15 16

temporal bone petrous part

strong

regional

strong expression: see section 01 02 03 05 21 22 moderate expression: see section 04

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 05 06 07 21 22 moderate expression: see section 08

scapula

strong

regional

strong expression: see section 01 02 03 04 moderate expression: see section 20 21 22

pelvic girdle skeleton

strong

regional

strong expression: see section 07 09 15 16 moderate expression: see section 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31834
Entity Detected:	Caly, calcyon neuron-specific vesicular protein ( MGI:1915816)
Sequence:	sense strand is shown >T31834 TGGGACGAGGACCATCATCCACCATGGTGAAGCTAGGCTGCAGCTTCTCAGGGAAGCCAGGCAAGGAAGC AGGAGACCAGGATGGGGCTGCCATGGACAGCGTCCCTCTGATCAGCCCCCTGGATGTCAGCCAGCTTCAG CCGTCATTCTCAGACCAGGTGGTCATCAACACACAGACAGAATATCAACTTACCTCTGCAGACCAGCCAA AGAAGTTTGCAGATTTGGAGGGTCAGAGGCTGGCTTGCAGTCACTCAGAGGAAGGACGCAGACTGCCCAC CGCAAGGATGATCGCTTTTGCTATGGCACTTCTGGGCTGTGTGCTGATCATGTACAAGGCCATCTGGTAT GACCAGTTTACCTGCCCAGATGGCTTCCTACTTCGGCACAAGATCTGCACCCCACTGACCCTGGAGATGT ACTACACCGAGATGGACCCCGAACGCCACCGCAGCATCCTGGCAGCCATCGGGGCCTACCCACTGAGCCG CAAACACGGCACTGAGATGCCCGCTGTCTGGGGAAACAACTACCGGACCGCCAAGGAGGAGCATAAGGGC ACTACCCCTGCGGCGATGGCTGTGTCCACTGCAGCTGCAGCTGCAGCGGCAGAGGGCACCGAGCCGTCAG GGAAGTCTTTGGACACGAGAGAGAAAGAAGACCCGCAGAAGGCGGAGGGCGTGCCATCCCAGCCCCCCAA GTGACTGTCTGCCCCGTCCCCGCTCCGCCCGTGACTGGGCATCCCTCACCAGCTACTTTGACATGCACAC TGCCTGACGCTTCTCCACCCAGT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6532801), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 61055. Forward Primer - name:061055_F_IRAW1_e02_Drd1ip, sequence:TGGGACGAGGACCATCAT; Reverse Primer - name:061055_R_SP6_IRAW1_e02_Drd1ip, sequence:CACTGGGTGGAGAAGCGT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18410 same embryo
	EMAGE:18409 same embryo
	EurExpress:euxassay_014371 same experiment
	MGI:4823598 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source