EMAGE mouse gene expression spatial database

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EMAGE:19046

Gcap14 granule cell antiserum positive 14 ( MGI:101859)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_015852. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_015852_01	euxassay_015852_02	euxassay_015852_03	euxassay_015852_04

euxassay_015852_05	euxassay_015852_06	euxassay_015852_07	euxassay_015852_08	euxassay_015852_09

euxassay_015852_10	euxassay_015852_11	euxassay_015852_12	euxassay_015852_13	euxassay_015852_14

euxassay_015852_15	euxassay_015852_16	euxassay_015852_17	euxassay_015852_18	euxassay_015852_19

euxassay_015852_20	euxassay_015852_21	euxassay_015852_22	euxassay_015852_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19046_wholemount_strong.wlz

19046_wholemount_moderate.wlz

19046_wholemount_weak.wlz

19046_wholemount_possible.wlz

19046_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19046_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 09 10 11 12

rest of cerebellum marginal layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 10 11 12 13 weak expression: see section 14 15 16 17 18

facial vii ganglion

strong

regional

strong expression: see section 03 04 17 18

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 05 16 moderate expression: see section 04 15

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 16 17 18 19 20 moderate expression: see section 08

vagus x ganglion

moderate

regional

moderate expression: see section 06

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 16 moderate expression: see section 04 06 15

dorsal root ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 13 14 15 16

neural retina

weak

regional

weak expression: see section 01 02 03 04 21 22 23

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 12 15 16

vomeronasal organ

weak

regional

weak expression: see section 12 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T40505
Entity Detected:	Gcap14, granule cell antiserum positive 14 ( MGI:101859)
Sequence:	sense strand is shown >T40505 GGGAACAGATCTTCTGGTAACGTGCAGAAGCCAAGAGTGAACTCCTGTGCCAGCAGAAGTAGTTCTGGAG AAAGCTTAGCACAGTCCCCAGACAATGCTAAATCTATCACTTGTGAAAAAATGGTAAGGTCACAAAGTTT TTCACATTCCATTCAGAATGTCTTCCTTCCACCTTCATCTATAACCAGATCGCATTCCTTCAACAGAGCT GTAGACCTTACAAAGCCTTATCAGAACCAACAGCTCCCCGTTCGAGTGCCTCTCCGGTCAGGTATGCTAA CAAGAAGTTCTCTGCAGTCAGAAGTACTCAATGGGAACGAACATGTGGGGTTTGGTTTTAATAGGCCTTA TGCTGCTGCTGGAAAGAAACTGGCCTTATCCAATGGCCCCGCTGTCTCGTCCACGTTGGTTTATAGGATG GCTCATCCCCCTCTACTGAAATCTAGCCGACCTCCATTTTCTGGGCCCATGACAGTTGACAGTAATAAAA ACCCACCTGCTGACATGTGCGTAGAGGAAGAAGGTATGGTTTCAGCCCAGGACAGCTCTCCGGGTAAGGA CCAAGAACTAATTGAAAATGAAAGTTACCGAAGAGACAATGACCAGACTGGTAAGAACGAGTCTAAAGTT AGGTACCTGAGCGATGACGTGGATGATATTTCCTTGTCGTCCTTGTCGTCTTCCGACAAGAATGATTTAA GTGAAGATTTCAGCGATGATTTCATAGACTTAGAGGACTCCAACAGAACTAGAATAACGCCAGAGGAAAT GACTCTCAAA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 158707. Forward Primer - name:158707_F_cDNA_Mm.53849, sequence:GGGAACAGATCTTCTGGTAACG; Reverse Primer - name:158707_N_SP6_cDNA_Mm.53849, sequence:TTTGAGAGTCATTTCCTCTGGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19045 same embryo
	EurExpress:euxassay_015852 same experiment
	MGI:4825016 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source