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EMAGE:19359

Anxa5 annexin A5 ( MGI:106008)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19359 EMAGE:19359 EMAGE:19359 EMAGE:19359 EMAGE:19359
"Pseudo-wholemount" of euxassay_002158. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_002158_01 euxassay_002158_02 euxassay_002158_03 euxassay_002158_04
EMAGE:19359 EMAGE:19359 EMAGE:19359 EMAGE:19359 EMAGE:19359
euxassay_002158_05 euxassay_002158_06 euxassay_002158_07 euxassay_002158_08 euxassay_002158_09
EMAGE:19359 EMAGE:19359 EMAGE:19359 EMAGE:19359 EMAGE:19359
euxassay_002158_10 euxassay_002158_11 euxassay_002158_12 euxassay_002158_13 euxassay_002158_14
EMAGE:19359 EMAGE:19359 EMAGE:19359 EMAGE:19359
euxassay_002158_15 euxassay_002158_16 euxassay_002158_17 euxassay_002158_18

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19359Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19359_wholemount_strong.wlz
19359_wholemount_moderate.wlz
19359_wholemount_weak.wlz
19359_wholemount_possible.wlz
19359_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19359_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
body cavity or lining
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18
limb
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 05 15 16 17 18
gland
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17
alimentary system
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 06 07 08 09 10 11 12 13 14 15 16 17 18
integumental system
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18
diencephalon meninges
moderate moderate
homogeneousmoderate expression: see section 04 05 06 07 08 09 10 11 12
telencephalon meninges
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18
hindbrain meninges
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14
midbrain meninges
moderate moderate
homogeneousmoderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13
spinal cord meninges
moderate moderate
homogeneousmoderate expression: see section 02 03 04 05 06 07 08 09
cardiovascular system
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 05 06 07 08 10 11 12 13 14
urinary system
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18
reproductive system
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18
respiratory system
moderate moderate
homogeneousmoderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18
tail
moderate moderate
homogeneousmoderate expression: see section 08 09 10 11 12
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6033
Entity Detected:Anxa5, annexin A5 ( MGI:106008)
Sequence:sense strand is shown

>T6033
CCCACGCGTCCGCCCACGCGTCCGACGGAGTTGTGAAGAGTACTGCCTTGTCCTTTGGCGTGTGCATCGG
TCCTGCTCTCACCCGCAGCCTGCGCTCTTCCGCCTGCTCCAGTCCACTCCTGACCGACAGCATCATGGCT
ACGAGAGGCACTGTGACTGACTTCCCTGGATTTGATGGCAGGGCTGATGCAGAAGTCCTTCGGAAGGCCA
TGAAAGGCTTGGGTACCGATGAGGACAGCATCCTGAACCTGTTGACATCCCGAAGCAATGCTCAGCGCCA
GGAAATTGCTCAGGAGTTTAAGACTCTGTTTGGCAGGGACCTTGTGGATGACCTGAAGTCTGAACTGACT
GGAAAGTTTGAGAAGTTAATTGTGGCTATGATGAAGCCCTCACGACTCTACGATGCCTACGAGCTGAAGC
ATGCTCTTAAGGGAGCTGGTACAGATGAGAAAGTATTGACCGAGATTATTGCTTCAAGGACACCTGAAGA
ACTCAGTGCCATAAAACAAGTTTATGAAGAAGAATATGGTTCCAACCTGGAAGATGATGTGGTGGGGGAT
ACTTCAGGGTACTACCAAAGGATGTTGGTGGTCCTCCTTCAGGGGAATAGAGACCCTGATACTGCAATTG
ATGATGCTCAAGTTGAACTGGATGCTCAGGCATTGTTCCAGGCTGGAGAGCTGAAGTGGGGGACAGATGA
AGAAAAATTCATCACCATCTTTGGGACACGCAGTGTGTCTCATTTAAGAAGAGTGTTTGACAAGTACATG
ACCATATCAGGATTTCAGATTGAGGAAACCATTGATCGGGAGACCTCGGGGAACTTGGAGCAGCTGCTCC
TGGCTGTTGTGAAGTCTATTCGGAGCATACCTGCCTACCTTGCAGAGACCCTCTACTATGCCATGAAGGG
TGCTGGGACGGACGATCACACCCTCATCAGAGTCGTGGTGTCGAGGAGTGAGATTGACCTGTTTAACATA
AGGAAGGAGTTTAGGAAGAACTTCGCCACCTCCCTGTACTCTATGATCAAGGGCGACACATCTGGAGACT
ATAAGAAGGCCCTGCTGCTGCTCTGCGGGGGCGAGGATGACTGAGAGCCGCCTGGAGCGCCCTGTGCCTG
CTGCCCACCATCAGCTTCCCTCAGCACCGCGCTTGCTTGCATTCTGTGCCTGCCTTACACTAGTATGCTA
ATGACCAAAGGTGTCTTAGATGAAAGCAGTGTCCCGCCCTTCTGATGTTCTGTCTACAGTAGGTAGTGAC
CTTCCCATGTCTCTCAGTCCAATGTCTGAGTTATTAATATTGTCTATGAATTGCTTTTTATATCCTTTTT
TAAAGCTTCATTTATATTAAGTTAATAACCCTATTATCTTGATTGGAACCTTAGCCGTGAAATTGTGAAC
TCTTGGAAATGCTGTCAATCAAGCTTACTGCTCTAGCAGACCTGCAAAATTATGATGGTAGTATCCAAAA
CATTGGCAACAAATAAAAACATTCCCTTCCCTCCAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3488901 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3488901 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19364 same embryo
 EMAGE:19360 same embryo
 EMAGE:19361 same embryo
 EMAGE:19362 same embryo
 EMAGE:19363 same embryo
 EurExpress:euxassay_002158 same experiment
 MGI:4823154 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS