EMAGE mouse gene expression spatial database

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EMAGE:19359

Anxa5 annexin A5 ( MGI:106008)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002158. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002158_01	euxassay_002158_02	euxassay_002158_03	euxassay_002158_04

euxassay_002158_05	euxassay_002158_06	euxassay_002158_07	euxassay_002158_08	euxassay_002158_09

euxassay_002158_10	euxassay_002158_11	euxassay_002158_12	euxassay_002158_13	euxassay_002158_14

euxassay_002158_15	euxassay_002158_16	euxassay_002158_17	euxassay_002158_18

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19359_wholemount_strong.wlz

19359_wholemount_moderate.wlz

19359_wholemount_weak.wlz

19359_wholemount_possible.wlz

19359_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19359_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

body cavity or lining

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

limb

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 15 16 17 18

gland

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17

alimentary system

moderate

homogeneous

moderate expression: see section 01 02 03 04 06 07 08 09 10 11 12 13 14 15 16 17 18

integumental system

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

diencephalon meninges

moderate

homogeneous

moderate expression: see section 04 05 06 07 08 09 10 11 12

telencephalon meninges

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

hindbrain meninges

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14

midbrain meninges

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13

spinal cord meninges

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09

cardiovascular system

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 10 11 12 13 14

urinary system

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

reproductive system

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

respiratory system

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

tail

moderate

homogeneous

moderate expression: see section 08 09 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6033
Entity Detected:	Anxa5, annexin A5 ( MGI:106008)
Sequence:	sense strand is shown >T6033 CCCACGCGTCCGCCCACGCGTCCGACGGAGTTGTGAAGAGTACTGCCTTGTCCTTTGGCGTGTGCATCGG TCCTGCTCTCACCCGCAGCCTGCGCTCTTCCGCCTGCTCCAGTCCACTCCTGACCGACAGCATCATGGCT ACGAGAGGCACTGTGACTGACTTCCCTGGATTTGATGGCAGGGCTGATGCAGAAGTCCTTCGGAAGGCCA TGAAAGGCTTGGGTACCGATGAGGACAGCATCCTGAACCTGTTGACATCCCGAAGCAATGCTCAGCGCCA GGAAATTGCTCAGGAGTTTAAGACTCTGTTTGGCAGGGACCTTGTGGATGACCTGAAGTCTGAACTGACT GGAAAGTTTGAGAAGTTAATTGTGGCTATGATGAAGCCCTCACGACTCTACGATGCCTACGAGCTGAAGC ATGCTCTTAAGGGAGCTGGTACAGATGAGAAAGTATTGACCGAGATTATTGCTTCAAGGACACCTGAAGA ACTCAGTGCCATAAAACAAGTTTATGAAGAAGAATATGGTTCCAACCTGGAAGATGATGTGGTGGGGGAT ACTTCAGGGTACTACCAAAGGATGTTGGTGGTCCTCCTTCAGGGGAATAGAGACCCTGATACTGCAATTG ATGATGCTCAAGTTGAACTGGATGCTCAGGCATTGTTCCAGGCTGGAGAGCTGAAGTGGGGGACAGATGA AGAAAAATTCATCACCATCTTTGGGACACGCAGTGTGTCTCATTTAAGAAGAGTGTTTGACAAGTACATG ACCATATCAGGATTTCAGATTGAGGAAACCATTGATCGGGAGACCTCGGGGAACTTGGAGCAGCTGCTCC TGGCTGTTGTGAAGTCTATTCGGAGCATACCTGCCTACCTTGCAGAGACCCTCTACTATGCCATGAAGGG TGCTGGGACGGACGATCACACCCTCATCAGAGTCGTGGTGTCGAGGAGTGAGATTGACCTGTTTAACATA AGGAAGGAGTTTAGGAAGAACTTCGCCACCTCCCTGTACTCTATGATCAAGGGCGACACATCTGGAGACT ATAAGAAGGCCCTGCTGCTGCTCTGCGGGGGCGAGGATGACTGAGAGCCGCCTGGAGCGCCCTGTGCCTG CTGCCCACCATCAGCTTCCCTCAGCACCGCGCTTGCTTGCATTCTGTGCCTGCCTTACACTAGTATGCTA ATGACCAAAGGTGTCTTAGATGAAAGCAGTGTCCCGCCCTTCTGATGTTCTGTCTACAGTAGGTAGTGAC CTTCCCATGTCTCTCAGTCCAATGTCTGAGTTATTAATATTGTCTATGAATTGCTTTTTATATCCTTTTT TAAAGCTTCATTTATATTAAGTTAATAACCCTATTATCTTGATTGGAACCTTAGCCGTGAAATTGTGAAC TCTTGGAAATGCTGTCAATCAAGCTTACTGCTCTAGCAGACCTGCAAAATTATGATGGTAGTATCCAAAA CATTGGCAACAAATAAAAACATTCCCTTCCCTCCAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3488901 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3488901 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19364 same embryo
	EMAGE:19360 same embryo
	EMAGE:19361 same embryo
	EMAGE:19362 same embryo
	EMAGE:19363 same embryo
	EurExpress:euxassay_002158 same experiment
	MGI:4823154 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source