EMAGE mouse gene expression spatial database

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EMAGE:19445

Tgfbr3 transforming growth factor, beta receptor III ( MGI:104637)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005198. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005198_01	euxassay_005198_02	euxassay_005198_03	euxassay_005198_04

euxassay_005198_05	euxassay_005198_06	euxassay_005198_07	euxassay_005198_08	euxassay_005198_09

euxassay_005198_10	euxassay_005198_11	euxassay_005198_12	euxassay_005198_13	euxassay_005198_14

euxassay_005198_15	euxassay_005198_16	euxassay_005198_17	euxassay_005198_18	euxassay_005198_19

euxassay_005198_20	euxassay_005198_21	euxassay_005198_22	euxassay_005198_23	euxassay_005198_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19445_wholemount_strong.wlz

19445_wholemount_moderate.wlz

19445_wholemount_weak.wlz

19445_wholemount_possible.wlz

19445_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19445_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 12 13 14 15 moderate expression: see section 04 05 06 07 08 09 10 11 16 17 18 19 20 21 22

mesenchyme

strong

regional

strong expression: see section 21 22 23 24 moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

diencephalon meninges

strong

regional

strong expression: see section 21 22 moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

choroid invagination

strong

regional

strong expression: see section 12 13 14 moderate expression: see section 07 08 09 10 11 16 17 18 19 20

telencephalon meninges

strong

regional

strong expression: see section 21 22 23 24 moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

hindbrain meninges

strong

regional

strong expression: see section 21 22 moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

metencephalon part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 12 13 14 15 moderate expression: see section 04 05 06 07 08 09 10 11 16 17 18 19 20 21 22

midbrain meninges

strong

regional

strong expression: see section 21 22 moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

spinal cord meninges

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15

brachial plexus

strong

regional

strong expression: see section 07 08 17 18

heart atrium

strong

spotted

strong expression: see section 07 08 09 10 11 12 15 16 17 18 19 20 21

heart ventricle

strong

spotted

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18

kidney calyx

strong

regional

strong expression: see section 06 07 08 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8830
Entity Detected:	Tgfbr3, transforming growth factor, beta receptor III ( MGI:104637)
Sequence:	sense strand is shown >T8830 CCGAGCGCCACTTTCCTCCTCCCTGGCAGTGAGAGAGAGAGAGAGGTGGAGAGGGAGAAGCGGGCTGCTG TCCTTCCAGGCCACTTTCGCTGCGCGATTCCGCGAGCTCCCGGCAGGAGGTGAAAGTCCCCGGCGGGGTC CGGATGGCGCAGTTGAACTGCGGCTGCTGAGCTCGCGGCCGCCTGCGCGCGCTGGGCGCGGGGACTCGTC TGGGCTACTCCCCCACCTCGCGTTGGACGACCGGTCCTGGACACACCGCTTGCGAGGCAAGTTGAACATT GCAGAGAAGGATCTTAAGGCTACACCCGACTTGCCACACTTGCCATCCAGCTGAAGAACCAAAGGCTGTT GGAGAGATGGCAGTGACATCCCACCACATGGTCCCTGTGTTTGTCCTGATGAGCGCCTGCCTGGCCACCG CAGGTCCAGAGCCCAGCACCCGGTGTGAACTGTCACCGATCAGTGCCTCTCATCCAGTCCAGGCCCTGAT GGAGAGCTTCACCGTTCTGTCTGGCTGTGCCAGCAGAGGCACCACTGGGCTGCCAAGGGAGGTTCACATC CTAAACCTCCGCAGTACAGACCAAGGACTAGGCCAGCCGCAGAGAGAGGTTACCCTGCATCTGAACCCCA TTGCCTCCGTGCACACTCACCACAAGCCTGTTGTGTTCCTGCTCAACTCCCCACAGCCCCTGGTGTGGCA TGTGAAGACAGAGAGACTGGCTGCTGGTGTCCCCAGACTCTTCCTGGTTTCGGAGGGTTCTGTGGTCCAG TTTTCATCAGGAAACTTCTCCTTGACAGCAGAAACAGAGGAAAGGAGTTTCCCTCAAGAAAATGAGCATC TGCTACACTGGGCCCAAAAGGAATATGGAGCAGTGACTTCATTCACCGAACTTAAGATAGCAAGAAATAT CTATATTAAAGTGGGAGAAGATCAAGTGTTCCCTCCCACGTGTAACATAGGGAAAAATTTTCTCTCGCTC AATTACCTTGCGGAGTACCTTCAACCCAAAGCCGCCGAAGGTTGTGTCCTGGCCAGTCAGCCCCACGAGA AGGAAGTGCATATCATTGAGTTAATCTCCCCCAACTCCAATCCTTACAGCACCTTCCAGGTGGATATAAT AATTGACATACGACCTGCTCGAGAGGATCCTGAGGTGGTCAAAAACCTCGTCCTGATCTTGAAGTGCAAA AAATCTGTCAACTGGGTGATCAAGTCTTTTGACGTCAAGGGAAACTTGAAAGTTATTGCTCCTGACAGTA TTGGCTTTGGAAAAGAGAGTGAACGATCCATGACAGTGACCAAATTGGTAAGAAATGACATCCCTTCCAC CCAAGAGAATCTGATGAAGTGGGCATTGGACAATGGCTACAGCCCAGTGACGTCATACACCATAGCTCCT GTGGCCAATAGATTTCATCTTCGGCTTGAGAACAACGAGGAGATGAGAGATGAGGAAGTCCACACCATTC CTCCTGAGCTTCGGATCCTGCTGGGCCCTGACCACCTGCCTGCCCTGGACAGCCCATCCTTCCAAGGGGA AATCCCAAATGGAGGTTTCCCCTTCCCATTCCCGGATATCCCCAGGAGAGGCTGGAAGGAGGGAGAAGAT AGGATCCCCCGGCCAAAGGAACCCATCATTCCCAGAGTTCAATTGCTTCCAGACCACAGAGAGCCAGAAG AAGTGCAAGGGGGCGTGAATATCGCCCTGTCAGTCAAATGTGACAATGAAAAGATGGTGGTAGCTGTAGA CAAAGATTCTTTCCAGACCAATGGCTACTCGGGGATGGAGCTCACCCTGTTGGATCCTTCCTGCAAAGCC AAGATGAATGGTACCCACTTTGTACTGGAGTCTCCGCTGAATGGCTGTGGTACTAGACATAGGAGGTCAG CCCCAGATGGTGTGGTTTACTATAACTCTATTGTGGTGCAGGCTCCATCCCCTGGGGATAGCAGTGGCTG GCCAGACGGCTACGAAGATTTGGAGTCGGGTGATAATGGATTTCCTGGAGACACAGATGAAGGAGAAACT GCCCCCCTGAGCCGTGCTGGAGTGGTAGTGTTTAACTGCAGCTTGCGGCAGCTGAGGAGTCCCAGTGGCT TCCAGGACCAGCTCGATGGAAATGCTACCTTCAACATGGAGCTGTATAACACAGACCTCTTTCTGGTGCC CTCCCCAGGGGTCTTCTCTGTGGCAGAGAATGAGCATGTATATGTTGAGGTGTCTGTCACTAAGGCTGAC CAAGATCTGGGATTTGCCATCCAAACCTGCTTTATCTCTCCATACTCAAACCCAGACAGAATGTCTGATT ACACCATCATCGAGAACATCTGTCCGAAGGATGACTCTGTGAAGTTCTACAGCTCCAAGAGAGTGCACTT CCCCATCCCACATGCTGAAGTGGACAAGAAGCGGTTCAGCTTTGTGTTCAAGTCCGTGTTCAACACCTCC CTGCTCTTCCTGCACTGCGAGCTGACGCTGTGCTCTAGGAAGAAGGGCTCCCAGAAGCTGCCAAAGTGTG TGACTCCTGACGACGCCTGCACCTCTCTAGATGCCACCATGATCTGGACCATGATGCAGAATAAGAAGAC ATTCACCAAGCCCCTGGCCGTGGTCCTCCAAGTAGATTATAAAGAAAATGTTCCAAACATGAAGGAGTCC AGTCCGGTTCCTCCTCCTCCACAGATTTTCCACGGCCTGGACACGCTCACCGTGATGGGCATTGCGTTTG CAGCATTTGTGATCGGAGCACTCCTGACGGGGGCCTTGTGGTATATCTACTCCCACACAGGGGAGACGGC ACGAAGGCAGCAAGTCCCTACCTCGCCACCAGCCTCGGAGAACAGCAGCGCAGCCCACAGCATAGGGAGC ACTCAGAGCACTCCCTGCTCTAGCAGCAGCACGGCCTAGGTGGACAGATGGACGCCGCTGCCTAGGTGGA CAGACGGATGCCTGCCACCGCCGGGGCAGGGCCAGATGCCGATGCTGGGTGTCCAGACTCAGAAGGCTTG ATCTGGGTTCCCTGTAAAGAGAGAGTGAATTTCAGTATACAGACAGCCTGCTCTACCCACCCCTTCCTTA CCACGGCCCACGTAAATGTGACCCTGGACATCTGTCACACGAAAGCTAAGCTGGTGGCCTTCCCCGCCAG CCCCTCGCAGGATGGGGGTTTCAATGTGAAACATATGCCAGTTTTTGTTCTTTTTTTTTTTTTTATGCTG CTTTGTCCAGGTGTCCAAACATCCATCATTTGGGGTCGTATGTTTTAAAGAGTAAAGGAGACGGTGAAGG GATGTCAGCTAGAGTGTAGAGCCAGGGAGAGACAGCCAGGGATTCTCGCCTAGCTGAACCAAGATGTAAA ATAGGAGAAATGCTCCCTCCATAGCCAATCTGCATCTCGCTCTGGATGCCCACTCCTGACCTGCCACGCC CTACATGGTCCCCTGTGTAGCTTGCTAGCCTTGGGGGCCTGTCACTTGCCAAAGTAACATTCCTCTCTTG GGCCAAAATGAATGCTGACTCCTGATACTCCGCACAAGCACACCAACTCCAAAAGTGCCTTAAAGCCAGG TTTGTGTCTCCTTTCAGGAGAGAGCGTGTAACGTCAGATTTGTGCCTGTCTCGAACATTCTGTCCTAATC ATACTTAAACATCAGGCAGGTGGGGTTCAGTGGTAAGCGAAGGGATTATTAGCAAGGTAATGCCTGTGTA TGGTCATATAGGCACAAATGCAGAACACCCAGCACATTGGAGAGACGACCACTTCCTCCTTTAAAATGTC CTATGCTTCCAGAGTCGGAACCTGAGTTATATGTAGATTATATTGACTCCAACCATCAGCCTTTCTGATG AGCCTGTAGAGAAGCCTAGAGAGCCCCGGGCCATCTGATGATGTCACCAGTGCTGCCTCAGATGATACAG AGACAGGGAGTTTCAGGTGAACTTCTTCAGCAGAATAAGGTGATGCCACTCTGACCTTTGCTGAGCTCAG TGACTCTTCAGTGTTAAAACTGAACCCCAGAGGGGATTCTCCATGCCAGATCCGCTGCAAATTAAATAAT ACCAATTTTCTAGAGTCCCTCCCTCCTCCCCCCTCCCCTGCCAACCAACTTAAGATTCCATAAGTAGAAC AAGCTAGATCCTAACCTGAAATAGCCATGAGCAAAGCCCACTGCCTTTACAGTCAGGCAGTTGTGCCCAG ACCTTCATGAATGAAAACGTCAACGTTGAAGCGTTGCTGATGGAAGCAGCGTGTGAAGGGATACGGACAA TCTCTCAGTCGATGAGCCCTCGTCTGACTGACCAGAGTGGCCCAGCTTCTATTCAATTGGGAAAATTTGA ACCAAAGCAGATTTCTTCAGGGCTGAGGGTCTCCAACCGATGCATAGATACATTGAAATACTTTAGATTT CCCACATGGATGGTTTGAGACTAGAGAAATACTTTTCTCTAGTTCGTTGGTTTTTAACATGAAGGTGTCT TTGGGGAGGACCTGGATCATGCTACCTGTGTTTAAAAATAATGCGCTTTGCCTAACCTTCAGCCGAATGT AACTGATAAGTATATTCTATGTATAAAAAAATGTTTAAAGATGTCTTCAAGGAAGACTAGAGTCTTTAAA TCACTTATAGCTATATTTTACTACAAAAATTCTGTATAAAGATATATTTAAATATTTTAGAGAAATTTAA GTTACTCCTTATGAAATGTTTAATTTCAGTTACTGTGAATTCTTCTAATCAGTTCGTTCTTTGATTTTAA AAAGAAAAACCTTTTCCATCATATTAAAGAGAAAATTAGCTATGGGAAGATATTAAAAGATTTCTTAGAT GGATGTGGTTAAAATTTTTGTAATATATTTAAATGTATATTTTTGAGAGAGATACTACATTGCCAAGGAC TTTATAAATAATCTTAAATTAACTTACAAATTTATAAGATGCAAACCAAAAGACTTTTTTTTTTTTAAAG AGAGAGAGAAAAGAGAAAAAAGCTTTTATTTCCTTGGATCTCATTATCAGAAATATTATTCAGCTCAAGA AATCTGACCCCATATTGGAAAATGAATGTCCTTTAGTATTCCTGAAGCTAAAGCTTGGGTGTCTCCTCCC AGCTCTTGTTACAGTGTTTCTGACTGCTTCACCCTTCACAGATTTAATACAATGTATAAGCCTTACTTTC ATAACATATTCTACAAGTTTCCTTAAGACTGTTGTTATAGTCTGGCTGAACCCATTTCCACCTGCAAAGT CTTGTGTTGTGTCTGCAGGCCACACCAGTGGCCACAGGGGCCATTTTGTCTTGTTGATGCTGAAACCATG CTGGACTGCCCCAGGCTAAGGCAGGACTGTCTGTGCAAGGCACTACAGCTATTCACGTGGAGTTACCGCT GCATTTTCAAAGGTCGGAGATCTTTCTTCTGCCTGAGTTGGTCATGTAGCAATTTTTTCCTAAACACAAA ATGGCCTTTTGCTTGTCTGGTAATGAGATTGTAAATCCATTTCCTGCGTGTGTTCTCCGCAGGCTTGCCT GAGTGTGTGGGGTGCGCAGGCGCGTGCCTGTCTTTTTGTACTGTAACTACCTCATGGTTTGAATGATGAG CGTACTGCTGGTTGTATTAACAGAGCACATTTGTCGGGTGAGTCGTGTATACAAACTTTTTTTTTTTATC CTGTTTGTTTTATCTGGGGGTTCGTTCATTAAACTGACAGATATTTTTCTAGGAAGGACTATTATCAGTT TCCAAATGCAATACTCTATTCTGGTTTTTCCTGAGTGAGCCTGATTTTGTTCCTGTTTTTCATCTACGAG GGTAAAAGCATACCCCGGGAAATCCCTGTGGCCAGTTTGAACACCCCTGCTGTAACCTTCCCTCTGTTCC ATGGTCTTTGTGAACCTGCTTAGAGGTATAGTTTTGATAACCACAGTTTTAATTCTTTTATCTGTGCATA ATAAAGATATGTATCAATTATTAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6410554 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6410554 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19441 same embryo
	EMAGE:19444 same embryo
	EMAGE:19442 same embryo
	EMAGE:19440 same embryo
	EMAGE:19443 same embryo
	EurExpress:euxassay_005198 same experiment
	MGI:4828681 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source