EMAGE mouse gene expression spatial database

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EMAGE:19452

Fxyd6 FXYD domain-containing ion transport regulator 6 ( MGI:1890226)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005187. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005187_01	euxassay_005187_02	euxassay_005187_03	euxassay_005187_04

euxassay_005187_05	euxassay_005187_06	euxassay_005187_07	euxassay_005187_08	euxassay_005187_09

euxassay_005187_10	euxassay_005187_11	euxassay_005187_12	euxassay_005187_13	euxassay_005187_14

euxassay_005187_15	euxassay_005187_16	euxassay_005187_17	euxassay_005187_18	euxassay_005187_19

euxassay_005187_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19452_wholemount_strong.wlz

19452_wholemount_moderate.wlz

19452_wholemount_weak.wlz

19452_wholemount_possible.wlz

19452_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19452_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

facial vii ganglion

moderate

regional

moderate expression: see section 02 05 16 17 weak expression: see section 04

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 05 16 17 18 weak expression: see section 04 15

vagus x ganglion

strong

regional

strong expression: see section 04 05 13

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 02 03 05 16 17 weak expression: see section 04 13 14

trigeminal v nerve

strong

regional

strong expression: see section 07 13 14

spinal cord

strong

regional

strong expression: see section 05 06 07 08 09 moderate expression: see section 04

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 10

cervical ganglion

strong

regional

strong expression: see section 05 12

thoracic ganglion

strong

regional

strong expression: see section 06 moderate expression: see section 07 08 09

heart valve

moderate

regional

moderate expression: see section 08 09 10

renal cortex

strong

regional

strong expression: see section 02 03 04 05 10 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9617
Entity Detected:	Fxyd6, FXYD domain-containing ion transport regulator 6 ( MGI:1890226)
Sequence:	sense strand is shown >T9617 GCCCCCAGCGCGCCACTCCCGGCTGCTTGGACTCTGCTAGTCCTTCTGGGATCCGCTCGGAGGGGACCCA ACGCCTAGACACCATGGAGACGGTGCTGGTCCTCTGCAGCTTGCTGGCCCCTGTGGTCCTGGCGAGTGCT GAGAAGGAGAAAGAAAAGGATCCTTTCTATTACGACTACCAGACCCTGAGGATTGGGGGGTTGGTGTTTG CTGTGGTCCTCTTCTCCGTTGGGATACTTCTCATCCTCAGTCGCAGGTGCAAGTGCAGTTTCAATCAGAA GCCCAGGGCTCCAGGTGACGAAGAGGCCCAGGTGGAGAACCTCATCACTACAAACGCTGCGGAGCCCCAG AAGGCAGAGAACTGAGGTGCAGCCTCCAGCGTGAAGCCTCCAGAAGCCAAGGCCAGCTACCTGACGCTTC AGAGACTGATGTCGAGGCTTGAGAAAACCGGCTACTTCATACCAGACCTTTCCCTAGGAGAAGCCAAGAA TGCGTGTTCCCTGTGCCTCTCCCTGCCCCCCACTAACCCCAGTCCTATATTAATGATGCAACACTTCTCA CTGCAGCCGGGCTCTGGACCCTGTCTGTGCCGTGTCTGTGCCGTGTCTGTGCCGTGTCTGTGTGTGTCTG CTGACTGTGGTCGACCGTGGATTTCATTGTTTCTGTGAACTGTGTGCTTCCTCTCCCACGCTACCATCGG CGCCTTGTGTCCTCCTGTGACCTCCATCTCCTCTTGCTCTCTGGAGTCTGCTCTGTTCCTGAGGAGCCCT AGTCCCATCTCTGGATTGAGAGAGAGTTAGTTGTCCCCAGGGCAGGAGTCCCCAGCGGACCCAGGGCTGA GAAAGTTTGTTGCTATTCTTTGTGGACTCTCAGAAGAAACTTGCTCTGGATTCCACCAGTTCAGAAGGTC TGTTTGCAGCTGGAGAGTCATTGCTGCAGGATCTGCCTTCCAGGGCTCCTTTTCCTTTTAGGGTGTCCAC TGCAGAGTCCCCATCTGCCCCACCCTTTAGGGAACACCCAGGAAATCTTGCCTCTGGGGAACGAGCTCCT CTCTTATCTTCCGAGCAATAACCTGGTAGGCTCTGGAGTCCCCCTGCCTTCATCCACCTTTCCTGCTTCT AAGACTTCCATCTCTGGCCCGATTTATCTGAGCAGAGGACTCCTTTCTTCTCCGAAGCTGGTCTCTAGCA GGAGATAGTCAAGGGGACAGGCAGTGGAAAGGGTGGGGACGGTTCTGTCTCTCTGCCTGGTGGTGTGGAT ATGGCAGACTTTGCAGTTTCTTGTCCCTGCCTGTCAGGGGTCAGAGTGGAGAGGGATGGAAGGGTTCAGG GGCAGCGGGGCTGAGGGTCAGAGAGCAACAGGGTATGAGCAGCCTGGATGCAAAGGCCAGAAGTCAAAAG TCATCAGAGTGGAAACTCACACCGGAGCCTGCCCAACCTGCCTAATGTGTTCCCTTGGAAACTAACCAAG CCACACTGTGTACTGGGACTGCTGATCTCTGTCTTGTGATCTATATCCTCTGACAACAGAAAAATAAATA AATACAGCATTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6531451 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6531451 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19454 same embryo
	EMAGE:19457 same embryo
	EMAGE:19455 same embryo
	EMAGE:19453 same embryo
	EMAGE:19456 same embryo
	EurExpress:euxassay_005187 same experiment
	MGI:4824950 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source