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EMAGE:19453

Fdps farnesyl diphosphate synthetase ( MGI:104888)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19453 EMAGE:19453 EMAGE:19453 EMAGE:19453 EMAGE:19453
"Pseudo-wholemount" of euxassay_005185. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005185_01 euxassay_005185_02 euxassay_005185_03 euxassay_005185_04
EMAGE:19453 EMAGE:19453 EMAGE:19453 EMAGE:19453 EMAGE:19453
euxassay_005185_05 euxassay_005185_06 euxassay_005185_07 euxassay_005185_08 euxassay_005185_09
EMAGE:19453 EMAGE:19453 EMAGE:19453 EMAGE:19453 EMAGE:19453
euxassay_005185_10 euxassay_005185_11 euxassay_005185_12 euxassay_005185_13 euxassay_005185_14
EMAGE:19453 EMAGE:19453 EMAGE:19453 EMAGE:19453 EMAGE:19453
euxassay_005185_15 euxassay_005185_16 euxassay_005185_17 euxassay_005185_18 euxassay_005185_19
EMAGE:19453
euxassay_005185_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19453Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19453_wholemount_strong.wlz
19453_wholemount_moderate.wlz
19453_wholemount_weak.wlz
19453_wholemount_possible.wlz
19453_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19453_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
limb
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 17 18 19 20
vertebral axis musculature
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
adrenal gland
strong strong
regionalstrong expression: see section 03 04 05 09 10 11 12
brain
strong strong
regionalstrong expression: see section 17 moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 18 19 20
adenohypophysis
moderate moderate
regionalmoderate expression: see section 09 10 11 weak expression: see section 07 08 12
vibrissa
strong strong
regionalstrong expression: see section 05 06 17 18 19 moderate expression: see section 04 07 08 20
facial vii ganglion
strong strong
regionalstrong expression: see section 03 04 05 15 16 17 moderate expression: see section 02
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 03 04 13
trigeminal v ganglion
strong strong
regionalstrong expression: see section 01 02 03 04 05 13 14 15 16 17 18
vagus x ganglion
strong strong
regionalstrong expression: see section 05 12
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 04 14 moderate expression: see section 05 13
spinal cord
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09
cervico-thoracic ganglion
strong strong
regionalstrong expression: see section 05 10
cervical ganglion
strong strong
regionalstrong expression: see section 05 12
thoracic ganglion
strong strong
regionalstrong expression: see section 06 07 08
dorsal root ganglion
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 10 11 12
retina
strong strong
regionalstrong expression: see section 01 02 03 20 moderate expression: see section 19
naris
strong strong
regionalstrong expression: see section 09 13 15 moderate expression: see section 10 11 14 weak expression: see section 12
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 06 09 11 12 13 15 16 17 moderate expression: see section 07 08 10 14
tongue
moderate moderate
regionalmoderate expression: see section 11
tongue muscle
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14
stomach
strong strong
regionalstrong expression: see section 05 06 moderate expression: see section 01 02 03 04
rectum
strong strong
regionalstrong expression: see section 08 09
midgut
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 14 15
mandible
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 12 13 14 15 16 17 18 19 moderate expression: see section 09 10 11
lower jaw incisor
strong strong
regionalstrong expression: see section 09 10 12 13
lower jaw molar
strong strong
regionalstrong expression: see section 06 07 15 16
maxilla
strong strong
regionalstrong expression: see section 04 05 06 07 08 12 14 15 16 17 18 moderate expression: see section 09 10 11 13 20 not examined expression: see section 19
upper jaw incisor
strong strong
regionalstrong expression: see section 09 10 13 14
upper jaw molar
strong strong
regionalstrong expression: see section 06 07 15 16
liver
strong strong
regionalstrong expression: see section 01 05 06 09 13 17 18 19 moderate expression: see section 02 03 04 07 08 10 11 12 14 15 16 20
metanephros
strong strong
spottedstrong expression: see section 02 03 04 05 10 11 12 13
lung
strong strong
regionalstrong expression: see section 01 05 06 09 13 17 18 moderate expression: see section 02 03 04 07 08 10 11 12 14 15 16
axial skeleton
strong strong
regionalstrong expression: see section 09 moderate expression: see section 06 07 08
orbito-sphenoid
strong strong
regionalstrong expression: see section 04 05 18 19 20 moderate expression: see section 01 02 03
tail mesenchyme
strong strong
regionalstrong expression: see section 08 09 10 12 13 moderate expression: see section 11
tail paraxial mesenchyme
strong strong
regionalstrong expression: see section 07
axial skeleton tail region
strong strong
regionalstrong expression: see section 09 10 moderate expression: see section 08 11 12 13
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T9618
Entity Detected:Fdps, farnesyl diphosphate synthetase ( MGI:104888)
Sequence:sense strand is shown

>T9618
AGAGTGCTGAGCGGATGCACGCTCAGCTTTTAGGTGTAAGCCGCAAACATCTTGGACCCCGGGAGAATCC
GCGTTGAAGAACAGAGCATTTAGCTCCTCTGTCAGAATGAATGGGAACCAGAAATTGGATGCTTATAACC
AAGAAAAGCAGAATTTCATCCAGCACTTCTCCCAGATCGTCAAGGTGCTGACTGAGAAGGAGCTGGGACA
CCCAGAGATAGGGGATGCTATTGCCCGGCTCAAGGAGGTCCTAGAGTACAATGCCTTAGGAGGCAAGTAC
AACCGGGGTTTGACCGTGGTACAAGCCTTCCAGGAGCTGGTGGAGCCGAAGAAACAGGATGCTGAGAGTC
TTCAGCGGGCCCTGACAGTGGGCTGGTGTGTAGAACTGCTCCAGGCTTTCTTCCTTGTGTCAGATGACAT
CATGGACTCTTCCCTCACTCGCCGGGGACAGATCTGCTGGTATCAGAAGCCAGGCATAGGCTTGGATGCC
ATCAACGACGCTCTGCTTCTGGAAGCCTCCATCTATCGTTTGCTGAAGTTCTACTGCAGGGAGCAGCCCT
ACTACCTGAACCTGCTGGAGCTCTTTCTGCAGAGTTCCTATCAGACAGAGATCGGGCAGACTCTAGACCT
CATGACAGCACCCCAGGGCCATGTGGATCTTGGTAGATACACTGAAAAGAGGTACAAATCGATTGTCAAG
TACAAGACGGCTTTCTACTCTTTCTACCTGCCTATTGCGGCCGCCATGTACATGGCAGGCATTGATGGGG
AGAAGGAACACGCCAATGCCCTGAAGATCCTGATGGAGATGGGCGAGTTCTTCCAGGTCCAGGACGACTA
CCTTGATCTCTTTGGAGACCCCAGTGTGACGGGAAAGGTCGGCACTGACATCCAGGACAACAAATGCAGC
TGGCTGGTGGTTCAGTGTCTGCTACGAGCCTCTCCTCAACAGCGCCAGATCTTAGAGGAGAATTATGGGC
AGAAGGACCCAGAAAAAGTGGCTCGGGTGAAAGCACTGTATGAGGCGCTGGATCTGCAGTCTGCTTTCTT
CAAGTATGAGGAAGACAGTTACAACCGCCTCAAGAGTCTCATAGAGCAGTGCTCTGCGCCCCTGCCCCCA
TCCATCTTCATGGAACTTGCAAACAAGATCTACAAACGGAGAAAGTGACCCCGGAATTGGAGAGGCTGGG
AGGGAGGGGGTCTCAATAAATTATTGTTCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6531586 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6531586 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19454 same embryo
 EMAGE:19457 same embryo
 EMAGE:19455 same embryo
 EMAGE:19452 same embryo
 EMAGE:19456 same embryo
 EurExpress:euxassay_005185 same experiment
 MGI:4824824 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS