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EMAGE:19483

Stxbp2 syntaxin binding protein 2 ( MGI:107370)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19483 EMAGE:19483 EMAGE:19483 EMAGE:19483 EMAGE:19483
"Pseudo-wholemount" of euxassay_004896. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004896_01 euxassay_004896_02 euxassay_004896_03 euxassay_004896_04
EMAGE:19483 EMAGE:19483 EMAGE:19483 EMAGE:19483 EMAGE:19483
euxassay_004896_05 euxassay_004896_06 euxassay_004896_07 euxassay_004896_08 euxassay_004896_09
EMAGE:19483 EMAGE:19483 EMAGE:19483 EMAGE:19483 EMAGE:19483
euxassay_004896_10 euxassay_004896_11 euxassay_004896_12 euxassay_004896_13 euxassay_004896_14
EMAGE:19483 EMAGE:19483 EMAGE:19483 EMAGE:19483 EMAGE:19483
euxassay_004896_15 euxassay_004896_16 euxassay_004896_17 euxassay_004896_18 euxassay_004896_19
EMAGE:19483 EMAGE:19483 EMAGE:19483 EMAGE:19483 EMAGE:19483
euxassay_004896_20 euxassay_004896_21 euxassay_004896_22 euxassay_004896_23 euxassay_004896_24
EMAGE:19483 EMAGE:19483
euxassay_004896_25 euxassay_004896_26

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19483Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19483_wholemount_strong.wlz
19483_wholemount_moderate.wlz
19483_wholemount_weak.wlz
19483_wholemount_possible.wlz
19483_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19483_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
thymus primordium
strong strong
regionalstrong expression: see section 10 11 13 14 moderate expression: see section 12
submandibular gland primordium
strong strong
regionalstrong expression: see section 06 07 08 09 16 17 18 19
pancreas
strong strong
regionalstrong expression: see section 09 10 11
thyroid gland
strong strong
homogeneousstrong expression: see section 10 13 14 15
adenohypophysis
strong strong
regionalstrong expression: see section 10 11 12 13 14 15 16
epidermis
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 22 23 24 25 moderate expression: see section 01 02 03 04 05 14 15 16 17 18 19 20 21 26
vibrissa
strong strong
regionalstrong expression: see section 06 07 08 09 22 23 24 moderate expression: see section 05 21
hypothalamus mantle layer
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14 15 16 17 18 19
diencephalon lateral wall mantle layer
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19
lateral ventricle choroid plexus
strong strong
regionalstrong expression: see section 18 19 20 moderate expression: see section 07 08 09 10 11 12 14 15 17
telencephalon mantle layer
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 16 17 18 19 20 21 22 23 24 25 26
telencephalon marginal layer
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
olfactory cortex mantle layer
moderate moderate
regionalmoderate expression: see section 11
olfactory cortex marginal layer
moderate moderate
regionalmoderate expression: see section 12 13 17 18 19
olfactory cortex ventricular layer
moderate moderate
regionalmoderate expression: see section 14
4th ventricle lateral recess
strong strong
regionalstrong expression: see section 18 19 20 21 moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17
medulla oblongata alar plate mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 14 15 16 17
medulla oblongata basal plate mantle layer
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14 15 16
pons mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19
midbrain mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19
ventral grey horn
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14 15
cochlea
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 16 17 18 19 20 21 22 moderate expression: see section 01
pharyngo-tympanic tube
strong strong
regionalstrong expression: see section 01 02 22 23 24 25
cornea epithelium
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 24 25 26
naris
strong strong
regionalstrong expression: see section 13 14 15 16 17 18
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 10 11 12 13 15 16 17 18 19 20
nasal cavity respiratory epithelium
strong strong
regionalstrong expression: see section 13 14 16 17 18
esophagus
strong strong
regionalstrong expression: see section 11 12 13
stomach
strong strong
regionalstrong expression: see section 02 05 06 07 08 09 10 moderate expression: see section 03 04
hindgut
strong strong
regionalstrong expression: see section 14 15
rectum
strong strong
regionalstrong expression: see section 14 15
midgut
strong strong
regionalstrong expression: see section 12 13 14 15 16 17 18
midgut loop
strong strong
regionalstrong expression: see section 11 12 13 14 15 16 17 18 19 20 21 22
liver left lobe
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12
liver right lobe
strong strong
regionalstrong expression: see section 13 14 15 16 17 18 19 20 21 22 23 24 25
urinary system
strong strong
regionalstrong expression: see section 04 05 20 21
bladder
strong strong
regionalExpression in the fundus region.
testis
weak weak
regionalweak expression: see section 05 06 07 08 18 19 20
urethra of male
strong strong
regionalstrong expression: see section 14 15 16
left lung
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 11 moderate expression: see section 02 03 04
right lung
strong strong
regionalstrong expression: see section 12 13 14 15 16 17 18 19 20 21 22
trachea
strong strong
regionalstrong expression: see section 12
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6877
Entity Detected:Stxbp2, syntaxin binding protein 2 ( MGI:107370)
Sequence:sense strand is shown

>T6877
GCTATTCCTCAGGGGAAGATGGCGCCCTTGGGGCTGAAGGCGGTGGTAGGGGAAAAAATTTTAAGCGGCG
TCATCCGGAGCGTTAAGAAGGATGGCGAGTGGAAGGTCCTCATCATGGATCACCCGAGCATGCGAATCTT
GTCATCCTGTTGTAAAATGTCAGATATCCTGGCGGAGGGCATCACCATCGTGGAAGACATCAACAAACGG
AGAGAACCCATTCCCAGCTTGGAGGCAATTTATTTGCTGAGTCCCACGGAGAAGTCGGTTCAGGCCCTGA
TTGCGGACTTCCAGGGAACACCAACCTTCACCTACAAAGCAGCCCATATCTTCTTCACTGACACATGCCC
TGAGCCCTTGTTCAGTGAACTGGGCCGCTCTCGCCTGGCGAAGGCGGTGAAGACATTGAAAGAGATCCAC
CTTGCCTTCCTCCCCTATGAGGCCCAGGTGTTCTCTCTGGATGCCCCACATAGCACCTACAACCTTTACT
GTCCATTCCGAGCAGGGGAGCGGGGGCGGCAGCTCGATGCACTGGCCCAGCAGATAGCCACACTGTGTGC
CACTCTTCAGGAATACCCATCCATCCGATACCGCAAGGGTCCAGAAGACACAGCCCAGCTGGCCCATGCT
GTCCTGGCCAAGCTGAACGCCTTCAAGGCAGACACTCCCAGTCTGGGCGAGGGCCCAGAGAAAACACGCT
CACAGCTACTCATAATGGACCGGGCAGCAGACCCCGTGTCCCCACTATTACACGAACTCACGTTCCAAGC
CATGGCCTATGATCTTCTGGACATCGAACAGGACACATACAGGTATGAGACCACAGGGCTGAGTGAGTCC
CGGGAGAAGGCTGTCCTCCTGGATGAAGATGATGACCTGTGGGTGGAACTTCGGCACATGCACATAGCAG
ATGTTTCCAAGAAGGTCACAGAACTCCTGAAGACATTCTGTGAGAGTAAGAGGCTGACCACAGACAAGGC
CAACATCAAAGACCTGTCCCACATCCTGAAAAAGATGCCACAGTATCAGAAGGAGCTGAACAAGTACTCT
ACGCACCTGCATTTGGCAGATGACTGCATGAAGCATTTCAAGGGCTCGGTGGAGAAGCTGTGCAGTGTGG
AGCAGGACCTGGCCATGGGCTCTGATGCAGAGGGTGAGAAGATCAAGGACGCCATGAAGCTGATTGTCCC
GGTGCTGCTGGATGCCTCGGTGCCACCCTACGACAAGATCCGGGTTCTGTTGCTCTACATCCTTCTGCGG
AATGGGGTGAGTGAGGAGAACCTGGCCAAGCTGATCCAGCATGCCAACGTGCAGTCGTACAGCAGCCTCA
TCCGGAATCTGGAGCAGCTGGGCGGCACTGTCACCAACTCCGCGGGCTCAGGGACCTCTAGCCGGCTGGA
GCGGAGAGAGCGCATGGAGCCCACCTACCAGCTGTCTCGCTGGTCCCCAGTCATCAAGGATGTGATGGAG
GATGTGGTGGAGGACCGGCTAGACCGAAAGCTGTGGCCCTTTGTGTCTGACCCTGCCCCTGTGCCTAGCT
CCCAGGCAGCTGTCAGCGCTCGCTTTGGCCACTGGCACAAAAACAAAGCTGGAGTAGAGGCCCGGGCTGG
GCCTAGGCTCATCGTGTACATTGTGGGTGGTGTCGCCATGTCGGAAATGAGGGCTGCCTATGAGGTGACC
AGGGCCACTGAGGGCAAGTGGGAGGTGCTCATAGGCTCTTCTCACATCCTTACTCCAACCCGCTTCCTCG
ATGACCTCAAGACACTGGATCAGAAGCTGGAGGGTGTGGCCCTGCCCTGACACAGCCCCTTGCCTCTGCC
CTCTCCTATTCCAGAGAAATAAACGCTTCCCTTCTGTCTTCCCAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3966319 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3966319 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19482 same embryo
 EMAGE:19481 same embryo
 EMAGE:19484 same embryo
 EMAGE:19485 same embryo
 EMAGE:19486 same embryo
 EurExpress:euxassay_004896 same experiment
 MGI:4828530 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS