EMAGE mouse gene expression spatial database

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EMAGE:19484

Polr2h polymerase (RNA) II (DNA directed) polypeptide H ( MGI:2384309)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004889. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004889_01	euxassay_004889_02	euxassay_004889_03	euxassay_004889_04

euxassay_004889_05	euxassay_004889_06	euxassay_004889_07	euxassay_004889_08	euxassay_004889_09

euxassay_004889_10	euxassay_004889_11	euxassay_004889_12	euxassay_004889_13	euxassay_004889_14

euxassay_004889_15	euxassay_004889_16	euxassay_004889_17	euxassay_004889_18	euxassay_004889_19

euxassay_004889_20	euxassay_004889_21	euxassay_004889_22	euxassay_004889_23	euxassay_004889_24

euxassay_004889_25	euxassay_004889_26	euxassay_004889_27

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19484_wholemount_strong.wlz

19484_wholemount_moderate.wlz

19484_wholemount_weak.wlz

19484_wholemount_possible.wlz

19484_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19484_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 10 11 12 13 14 15

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 10 17 18 19 20

hypothalamus ventricular layer

weak

regional

weak expression: see section 13 14 15

diencephalon lateral wall ventricular layer

weak

regional

weak expression: see section 13 14 15

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 12 13 18 19

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 16 17 18 19 20 21 22 23 24

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 09 10 15 16

pons mantle layer

weak

regional

weak expression: see section 08 18

midbrain ventricular layer

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15 16

renal cortex

moderate

regional

moderate expression: see section 06 07 08 09 10 16 17 18 19

testis

moderate

regional

moderate expression: see section 06 07 08 19 20 21 weak expression: see section 05

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6552
Entity Detected:	Polr2h, polymerase (RNA) II (DNA directed) polypeptide H ( MGI:2384309)
Sequence:	sense strand is shown >T6552 CTCTGCCCTGTCTTACTTTTCTCCACGTGAGGGGGTGCGCGAATCCCCAGTGGTGGCCGCGCGCTCACAT CCTCTGCAGCTCTCGTGCCCTTCGCGATGGCGGGCATCCTGTTCGAGGATATTTTCGATGTGAAAGACAT CGACCCAGAAGGCAAGAAATTTGACCGAGTATCCCGGCTGCACTGTGAGAGTGAATCTTTCAAGATGGAC CTCATCTTAGATGTAAACATTCAGATTTACCCTGTGGACTTAGGTGACAAGTTCCGGTTGGTCATAGCTA GTACTTTGTATGAAGATGGTACCTTGGATGATGGTGAATACAACCCCACAGATGACAGGCCTTCCAGGGC TGACCAGTTTGAGTATGTCATGTATGGGAAAGTGTACAGGATCGAAGGGGATGAGACCTCGACTGAAGCA GCCACACGCCTCTCTGCATACGTGTCCTATGGGGGCCTGCTCATGAGGCTTCAGGGTGATGCCAACAACC TGCATGGATTTGAGGTGGACTCCAGAGTTTATCTGCTGATGAAGAAGCTGGCCTTCTGAACTTCGTGGGA AACCAGACTTGCTGCTTAGTCACTCATGTCATGGACACTGTTCAAGGCTGTGTAGAAGCTGTCGGTGTTA GCCTGTAAAAGGGGCAGAACTCTGTCCACTGTGGGGACATCTTCAGTTTGAATGCAGTGGAAAACCGACT TGTCTGTAAAAGGCCGAGAGGGAGAACTTAGCACGAATCTGAAGTTGTATATGATTTTTTTTTTTATTAA AGTTTACTTTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3591968 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3591968 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19482 same embryo
	EMAGE:19481 same embryo
	EMAGE:19483 same embryo
	EMAGE:19485 same embryo
	EMAGE:19486 same embryo
	EurExpress:euxassay_004889 same experiment
	MGI:4827321 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source