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EMAGE:19492

Gpc4 glypican 4 ( MGI:104902)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19492 EMAGE:19492 EMAGE:19492 EMAGE:19492 EMAGE:19492
"Pseudo-wholemount" of euxassay_004882. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004882_01 euxassay_004882_02 euxassay_004882_03 euxassay_004882_04
EMAGE:19492 EMAGE:19492 EMAGE:19492 EMAGE:19492 EMAGE:19492
euxassay_004882_05 euxassay_004882_06 euxassay_004882_07 euxassay_004882_08 euxassay_004882_09
EMAGE:19492 EMAGE:19492 EMAGE:19492 EMAGE:19492 EMAGE:19492
euxassay_004882_10 euxassay_004882_11 euxassay_004882_12 euxassay_004882_13 euxassay_004882_14
EMAGE:19492 EMAGE:19492 EMAGE:19492 EMAGE:19492 EMAGE:19492
euxassay_004882_15 euxassay_004882_16 euxassay_004882_17 euxassay_004882_18 euxassay_004882_19
EMAGE:19492 EMAGE:19492 EMAGE:19492 EMAGE:19492 EMAGE:19492
euxassay_004882_20 euxassay_004882_21 euxassay_004882_22 euxassay_004882_23 euxassay_004882_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19492Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19492_wholemount_strong.wlz
19492_wholemount_moderate.wlz
19492_wholemount_weak.wlz
19492_wholemount_possible.wlz
19492_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19492_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
diaphragm
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 17 18 19 20 21 22 23 moderate expression: see section 07 08 09 10 15 16
hand
strong strong
regionalstrong expression: see section 01 02
hindlimb digit 1 mesenchyme
strong strong
regionalstrong expression: see section 08 09 23 24
hindlimb digit 2 mesenchyme
strong strong
regionalstrong expression: see section 09 10 23 24
hindlimb digit 3 mesenchyme
strong strong
regionalstrong expression: see section 09 10 23 24
hindlimb digit 4 mesenchyme
strong strong
regionalstrong expression: see section 09 10 22 23 24
hindlimb digit 5 mesenchyme
strong strong
regionalstrong expression: see section 09 10 22 23 24
foot mesenchyme
strong strong
regionalstrong expression: see section 22
vertebral axis musculature
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22 23 24
adrenal medulla
moderate moderate
regionalmoderate expression: see section 05 06 07 08 13 14 15
thymus primordium
moderate moderate
regionalmoderate expression: see section 09 weak expression: see section 10 11 12 13
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 06 07 08 09 15 16 17 18
pancreas
weak weak
regionalweak expression: see section 08 09 10
hypothalamus ventricular layer
strong strong
regionalstrong expression: see section 11 12 13 14 15
diencephalon lateral wall ventricular layer
strong strong
regionalstrong expression: see section 12 13 14
telencephalon mantle layer
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
olfactory cortex ventricular layer
strong strong
homogeneousstrong expression: see section 12 13 14 18 19
telencephalon ventricular layer
strong strong
homogeneousstrong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 16 17 18 19 20 21 22 23 24
medulla oblongata basal plate mantle layer
moderate moderate
single cellmoderate expression: see section 05 13 14
medulla oblongata basal plate ventricular layer
strong strong
regionalstrong expression: see section 08 09 10 moderate expression: see section 11 12 13
rest of cerebellum ventricular layer
strong strong
regionalstrong expression: see section 03 09 10 13 14 15 16 17 18 19 moderate expression: see section 04 05 06 07 08 11 12 weak expression: see section 20
pons ventricular layer
strong strong
regionalstrong expression: see section 09 10 13 14 15 16 moderate expression: see section 06 07 08 11 12
midbrain mantle layer
strong strong
single cellstrong expression: see section 05 06 07 08 13 14 15 17 moderate expression: see section 16 18
midbrain ventricular layer
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 14 15 17
trigeminal v nerve maxillary division
strong strong
regionalstrong expression: see section 08 09 22 moderate expression: see section 23
dorsal grey horn
strong strong
single cellstrong expression: see section 09 10 moderate expression: see section 06 07 08 11 12 13
ventral grey horn
moderate moderate
single cellmoderate expression: see section 07 08 10 12
spinal cord ventricular layer
strong strong
single cellstrong expression: see section 09 10
cochlea
moderate moderate
regionalmoderate expression: see section 17 18 19 20 21 weak expression: see section 03 05 06 07 16
pharyngo-tympanic tube
strong strong
regionalstrong expression: see section 01 02 22 23 moderate expression: see section 03 24
extrinsic ocular muscle
moderate moderate
regionalmoderate expression: see section 05 06 07 20 21 weak expression: see section 04 22 23
aorta
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12
tongue muscle
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16 17
stomach
moderate moderate
regionalmoderate expression: see section 01 05 06 07 08 09 weak expression: see section 02 03 04
hindgut
moderate moderate
regionalmoderate expression: see section 12 13
midgut
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14 15 16 17 18 19
mandible
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 17 18 19 20 21 22 23 weak expression: see section 10 11 15 16
maxilla
moderate moderate
regionalmoderate expression: see section 06 07 08 09
bladder
moderate moderate
regionalmoderate expression: see section 11 12 13 14 weak expression: see section 15
metanephros
strong strong
regionalstrong expression: see section 06 07 14 15 16 17 18 moderate expression: see section 05
genital tubercle of male
moderate moderate
regionalmoderate expression: see section 12 13 14 15 16 17
testis
moderate moderate
regionalmoderate expression: see section 05 18 19 20 weak expression: see section 04 06 07
lung
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 11 12 13 14 15 16 17 18 19 20 21
clavicle
moderate moderate
regionalmoderate expression: see section 08 15
sternum
strong strong
regionalstrong expression: see section 07 08 09 17 18 moderate expression: see section 16 weak expression: see section 10 14 15
tail mesenchyme
moderate moderate
regionalmoderate expression: see section 12 13 14 15 16 17 18
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6890
Entity Detected:Gpc4, glypican 4 ( MGI:104902)
Sequence:sense strand is shown

>T6890
CCTCCTTCACTACCAACTCCAACTCCCCGGTTCTCGCTCCGCTCGTGAGTGTTCACTCCGCGCCTCTCCG
CCCGCTCCGCAGCGCTGCTTTCCATCGGGTCTCATTCTGTCCCCTAAAGGTGGGAGCGCGTCCGTCAGGG
TCCGCACCATGGCACGCTTAGGCTTGCTCGCGCTCCTCTGCACCCTGGCCGCGCTCAGCGCCTCGCTGCT
GGCTGCGGAGCTCAAGTCGAAAAGTTGCTCGGAAGTGCGACGTCTCTACGTGTCCAAAGGCTTCAACAAG
AATGATGCCCCCCTCTATGAGATCAACGGTGACCATTTGAAGATCTGTCCCCAGGATTATACATGCTGTT
CTCAAGAGATGGAAGAGAAGTACAGCCTGCAAAGTAAAGATGATTTCAAAACCGTGGTCAGCGAACAGTG
CAACCATTTGCAAGCCATCTTTGCATCCCGTTACAAGAAGTTTGATGAATTCTTCAAAGAACTTCTTGAA
AATGCAGAGAAATCCCTGAATGATATGTTCGTGAAGACATATGGCCACTTATACATGCAAAATTCAGAGC
TATTTAAAGATCTCTTCGTTGAGTTGAAGCGCTACTATGTGGCGGGAAATGTGAACCTGGAAGAAATGTT
AAATGACTTCTGGGCTCGCCTTCTGGAGCGCATGTTTCGCCTGGTGAACTCCCAGTACCATTTTACAGAT
GAGTACTTGGAATGTGTGAGCAAATATACAGAGCAGCTGAAGCCTTTTGGAGATGTCCCTCGGAAACTGA
AGCTCCAGGTTACCCGCGCATTTGTTGCAGCCAGGACCTTCGCTCAAGGCTTAGCAGTTGCAAGGGATGT
AGTAAGCAAAGTGTCCGTGGTGAACCCCACAGCTCAGTGCACCCATGCACTGCTGAAGATGATCTACTGC
TCCCACTGCCGGGGCCTGGTGACTGTGAAGCCCTGTTACAACTATTGCTCAAACATCATGCGAGGCTGTT
TGGCCAACCAAGGAGACCTTGATTTTGAGTGGAACAATTTCATAGATGCTATGCTGATGGTGGCAGAGAG
GCTGGAGGGTCCTTTCAACATTGAGTCCGTTATGGATCCCATCGATGTGAAGATTTCTGATGCTATCATG
AATATGCAGGATAATAGTGTTCAAGTGTCTCAGAAGGTTTTCCAAGGCTGTGGCCCTCCGAAGCCTCTCC
CAGCTGGACGAATTTCTCGCTCCATCTCTGAAAGTGCCTTCAGTGCTCGATTCAGACCTTATCATCCAGA
GCAACGCCCAACCACGGCAGCTGGCACTAGTTTGGACCGACTGGTTACTGATGTCAAGGAGAAACTGAAA
CAAGCTAAGAAGTTCTGGTCCTCTCTCCCAAGCACCGTTTGCAATGATGAGAGGATGGCAGCAGGAAATG
AAAATGAGGATGACTGCTGGAATGGCAAAGGCAAAAGCAGGTACCTGTTTGCAGTGACAGGAAATGGATT
GGCCAACCAGGGCAACAACCCAGAAGTCCAGGTTGACACCAGCAAGCCAGACATACTGATCCTTCGTCAG
ATCATGGCCCTTCGGGTTATGACCAGTAAAATGAAGAATGCTTACAATGGAAATGACGTGGACTTCTTTG
ACATCAGTGATGAGAGTAGTGGAGAGGGAAGTGGAAGCGGATGTGAATATCAGCAGTGCCCTTCGGAGTT
CGAGTACAACGCCACTGACCATTCTGGGAAGAGTGCCAACGAGAAAGCTGACAGTGCCGGTGGTGCCCAT
GCAGAGGCAAAGCCCTACCTCCTCGCTGCTCTCTGCATCCTGTTTCTCGCTGTGCAGGGAGAGTGGAGAT
AATTGTCAAACTCTGAGTAAAAGCGTTCATCATCAGAAAGTTCAAAGGCACCAAGTTCTCACTTTTTTTT
TTTTTAACCATCCTAGTGACTTTGCTTTTTAAATGAATGGACAACAATGTACAGTTTTTACTGTGTGGCC
ACTGGTTTAAGCAATGTTGACTTTGTTTTGCTCATTCAGTTTGAGGGGACAGCGGGGCCTCCTGCCTTTA
AGTTTGTTCCTCCCCCTCACCTCCCCCCTCCTTTGAGACTCGTGTTCTGCGTGGCTAGCAGTGTAGGTAC
AGAACTGTAGTTAGTTGTGCATTGGTGATCTTGTTTTCTTTGTTGGCTTCTCTCATTTTATTTGTGGGGT
TTTTCTTTTCCAATTATGGTCTCACCTTGTTTCTCACAAGAAAACCAGGGTCCTTTCTTGGCATGTAACA
TGTACGTATTTCTGAAATATTAAATAGCTGTACAGAAGCAGGTTTATTTATCATGTTATCTTATTAAAAG
AAAAAGCCCAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3967797 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3967797 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19489 same embryo
 EMAGE:19491 same embryo
 EMAGE:19490 same embryo
 EMAGE:19488 same embryo
 EMAGE:19487 same embryo
 EurExpress:euxassay_004882 same experiment
 MGI:4825157 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS