EMAGE mouse gene expression spatial database

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EMAGE:19523

Aacs acetoacetyl-CoA synthetase ( MGI:1926144)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005036. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005036_01	euxassay_005036_02	euxassay_005036_03	euxassay_005036_04

euxassay_005036_05	euxassay_005036_06	euxassay_005036_07	euxassay_005036_08	euxassay_005036_09

euxassay_005036_10	euxassay_005036_11	euxassay_005036_12	euxassay_005036_13	euxassay_005036_14

euxassay_005036_15	euxassay_005036_16	euxassay_005036_17	euxassay_005036_18	euxassay_005036_19

euxassay_005036_20	euxassay_005036_21	euxassay_005036_22	euxassay_005036_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19523_wholemount_strong.wlz

19523_wholemount_moderate.wlz

19523_wholemount_weak.wlz

19523_wholemount_possible.wlz

19523_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19523_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 10 11 12 13

brain

moderate

regional

moderate expression: see section 01 03 04 05 weak expression: see section 02 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

facial vii ganglion

strong

regional

strong expression: see section 04 05 18 19

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 16 moderate expression: see section 17

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 16 17 18 19 20 21 moderate expression: see section 03

vagus x ganglion

strong

regional

strong expression: see section 07 16

trigeminal v nerve

strong

regional

strong expression: see section 08 16

spinal cord

weak

regional

weak expression: see section 08 09 10 11 12 13 14

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 08 11 12 14

cervical ganglion

moderate

regional

moderate expression: see section 07 15

dorsal root ganglion

strong

regional

strong expression: see section 08 09 14 15 16 moderate expression: see section 06 07 10 13

not examined

regional

not examined expression: see section 01 02 03 21 22 23

neural retina

strong

regional

strong expression: see section 01 21 22 moderate expression: see section 02 23 weak expression: see section 03

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7447
Entity Detected:	Aacs, acetoacetyl-CoA synthetase ( MGI:1926144)
Sequence:	sense strand is shown >T7447 CGGACGCGTGGGCGGCGCACCGCTGATCCGCTCCACGCCTTGCGCTCTCCGCTCTCAGCCAAAGCCCGGC AGCCCCGGCCACGCAGCTCCGCAACCATGTCCAAGCTGGCGCGGCTCGAGCGCGAGGAGATCATGGAGTG CCAGGTGATGTGGGAGCCTGACAGCAAGAAGGACACGCAGATGGACCGCTTCCGGGCGGCCGTGGGTACC GCCTGCGGCCTGGCGCTTGGGAATTACAATGACTTATACCACTGGTCTGTCCGGTCGTATATGGACTTTT GGGCTGAGTTCTGGAAGTTCAGTGGAATCGTCTACTCACGCATGTATGATGAGGTTGTGGACACATCCAA AGGGATTGCAGATGTCCCCGAGTGGTTCAGAGGCAGCCGCCTCAACTACGCAGAGAACCTCCTGCGGCAC AAGGAGAACGACAGAGTCGCCCTTTACGTGGCCCGGGAAGGCAGAGAGGAGATCGTGAAGGTGACTTTTG AAGAGCTGCGGCAGCAGGTGGCTCTGTTCGCAGCTGCCATGAGGAAGATGGGCGTGAAGAAAGGGGACCG TGTGGTCGGCTATCTACCCAACAGTGCGCATGCGGTGGAGGCCATGCTGGCTGCTGCCAGTATTGGGGCC ATTTGGAGTTCTACCTCGCCGGACTTTGGTGTAAATGGTGTCCTGGACCGCTTTTCTCAAATTCAGCCGA AACTTATCTTCTCGGTGGAGGCTGTTGTCTACAATGGCAAGGAGCATGGTCACCTGGAGAAGCTGCAGCG AGTTGTGAAAGGACTGCCTGACCTGCAGCGAGTGGTGCTGATCCCCTATGTCCTCCCAAGGGAGAAGATT GACATTTCCAAGATCCCCAACAGTGTGTTCCTGGATGATTTCCTGGCAAGCGGGACCGGCGCGCAGGCGC CGCAGCTCGAGTTTGAGCAGCTGCCCTTCAGCCACCCTCTGTTCATCATGTTCTCCTCAGGCACGACGGG AGCGCCCAAGTGCATGGTGCACTCAGCCGGGGGCACCCTCATCCAGCACCTGAAGGAGCACATGCTACAC GGCAACATGACAAGCAGTGACATCCTGCTCTACTACACCACGGTCGGCTGGATGATGTGGAACTGGATGG TGTCAGCCCTGGCCACAGGAGCATCCTTGGTCCTGTATGATGGCTCCCCGCTGGTTCCGACCCCCAACGT GTTGTGGGACCTTGTGGACAGGATAGGGATCACCATCCTGGGAACGGGAGCCAAGTGGCTGTCAGTGCTG GAGGAGAAGGACATGAAGCCAGTGGAAACTCACAACCTCCACACGCTGCACACGATCCTGTCCACCGGCT CGCCGCTGAAAGCCCAGAGTTACGAGTATGTGTACAGATGCATCAAGAGCTCCGTGCTCCTGGGCTCCAT CTCAGGAGGCACTGACATCATCTCCTGTTTCATGGGCCAGAACTCCTCTATTCCTGTGTACAAGGGTGAG ATCCAAGCCCGGAACCTTGGCATGGCTGTGGAAGCCTGGGACGAGGAAGGGAAGGCCGTCTGGGGAGCGA GTGGCGAGCTGGTGTGCACTAAGCCCATTCCCTGCCAGCCCACGCACTTCTGGAACGACGAGAACGGCAG CAAGTACCGGAAGGCTTACTTCTCCAAATTCCCAGGTGTCTGGGCACACGGTGACTACTGCAGGATCAAC CCCAAAACAGGAGGCATTATCATGCTGGGCCGTAGTGATGGCACCCTCAACCCCAATGGCGTCCGCTTTG GCAGCTCGGAGATCTACAACATCGTGGAAGCCTTCGATGAGGTGGAGGACAGCCTGTGTGTACCCCAGTA CAACAGAGATGGCGAGGAGCGGGTGGTCCTGTTCCTGAAGATGGCGTCCGGGCACACTTTCCAGCCTGAC CTCGTGAAGCGCATCCGAGACGCCATCCGACTTGGCCTGTCTGCCCGCCATGTGCCCAGCCTCATCCTGG AGACCCGAGGCATTCCATACACACTCAATGGCAAGAAAGTGGAGGTGGCCGTGAAGCAGGTGATGGCTGG GAGGACTGTGGAGCACCGGGGGGCCTTCTCCAACCCCGAGACCCTCGACCTGTACCGGGACATCCCTGAG CTGCAGGACTTCTGAGCCAGCAGCTCGCACTCCATCCAGCTGTGCATGTGATGGAACTTAGGGACACTTT AGAGACAACAGCTGCTCCAGGTGGCCCTGGCACTGCACACTCCACAGGCTCAGGAACAGCTTTCCTCTTT CGGAGTCATTGGTGGGGGCCAGGTCTCCCGAGCTCCAGGATGTGCCTGGCCTTCGGAGACCCTAAGCATA CACTGAGGCTTGTCTCGGCCCTGCTGCTATAGGTCAGCACACAGCCTTGCAGGGGCAGGGCTGGTGACAT TTGGTGACAGCACACTGGAGGAGCGATGTGGCCTTCAGCCTCATGAGCCCGCTGTTGTATCTCGCTCTAT GAAGGTGAAGTCTTACCTATACTCTCTGTCCTTAACTGGCCTGGCTTCCTCAGCGTTACTGTCTTCTCAA GAGCTGACTAAAGCCAGGGCACCTGGCCCAGCATGCCTGTCACCTCACAGCTGGCTGTTGGGACACCTGG GCTGACGGCTCCTGTACCGTCTACCAAGGCCAGAACACCATTCTAGGGAATGGTGAGTGAATTGTCCAGA CAGCCAAGAGCTCTCTGCTATGGCAAGTCCCCTCTGCTGTCGGGTGTCCCCGACTGTTTCTGGGTCTTAC CAGCACAGTGTTTGAATGGGTCAAACGGTTTAATGTTAATTGAGGGCCTGGGGGCACTTGAATCCCTGGG TGTCTACCCCAGGTTACCACTTAGCTGTGTGCTGGGCAGTGTGATCCTGGAGTCCGCCAGGGATGGAGCT GCCCCGCCTCAGAGGGAAATGGCCCCGTGCTCCCTCCTAATAGTTCCTGGGCTCTTTCTATTGTGACTGT CCTCTACATTTGTAAATGAAGTCTCAGGCTTTGGCTGCCAGGACTTAATGGTGCTTCTCCTCTAGCACAC GGAATATGTCATAAATCCTGGTGATTGCTTTTGTATTTTTTTTTTTAAAAACTGAGCACAACATAAAACC TTTTAAAGATATCCTGGATCTTAAGTCTATAAAGGAAAAGTGCTATGAAGAATTTTATGGAATAAATCTG TGCCATGCACACCTGTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4235112 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4235112 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19525 same embryo
	EMAGE:19526 same embryo
	EMAGE:19522 same embryo
	EMAGE:19524 same embryo
	EurExpress:euxassay_005036 same experiment
	MGI:4822850 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source