EMAGE mouse gene expression spatial database

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EMAGE:19525

Fastkd5 FAST kinase domains 5 ( MGI:2139469)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004980. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004980_01	euxassay_004980_02	euxassay_004980_03	euxassay_004980_04

euxassay_004980_05	euxassay_004980_06	euxassay_004980_07	euxassay_004980_08	euxassay_004980_09

euxassay_004980_10	euxassay_004980_11	euxassay_004980_12	euxassay_004980_13	euxassay_004980_14

euxassay_004980_15	euxassay_004980_16	euxassay_004980_17	euxassay_004980_18	euxassay_004980_19

euxassay_004980_20	euxassay_004980_21	euxassay_004980_22	euxassay_004980_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19525_wholemount_strong.wlz

19525_wholemount_moderate.wlz

19525_wholemount_weak.wlz

19525_wholemount_possible.wlz

19525_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19525_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

moderate

regional

moderate expression: see section 05 16 weak expression: see section 01 02 03 04 06 07 08 10 17 18 19 20 21

thymus primordium

moderate

regional

moderate expression: see section 10 11 12 13 weak expression: see section 14

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 16 17 18

thyroid gland

moderate

regional

moderate expression: see section 10

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 12 13 14

olfactory lobe

moderate

regional

moderate expression: see section 07

telencephalon ventricular layer

moderate

regional

moderate expression: see section 02 03 04 05 06 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

4th ventricle

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midbrain ventricular layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 17 18 19 20 21

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 07 08 17 18 19

spinal cord

moderate

regional

moderate expression: see section 12 13 14 weak expression: see section 10 11

cervico-thoracic ganglion

weak

regional

weak expression: see section 12 15

cervical ganglion

moderate

regional

moderate expression: see section 08 16

thoracic ganglion

weak

regional

weak expression: see section 11 12

dorsal root ganglion

weak

regional

weak expression: see section 08 09 10 15 16

naris

moderate

regional

moderate expression: see section 16 weak expression: see section 15

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 11 12 14 15 16 17 18 weak expression: see section 10

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 11 12 16 weak expression: see section 14 15

tongue

moderate

regional

moderate expression: see section 11 12 13 14 16 weak expression: see section 10 15

lower jaw molar

moderate

regional

moderate expression: see section 07 19 weak expression: see section 18

upper jaw molar

moderate

regional

moderate expression: see section 07 19 weak expression: see section 18

hepatic duct

moderate

regional

moderate expression: see section 04

liver

moderate

regional

moderate expression: see section 01 02 03 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

metanephros

moderate

regional

moderate expression: see section 07 08 09 15 16 17 18 19

lung

moderate

regional

moderate expression: see section 05 06 07 08 09 10 15 16 17 18 19 weak expression: see section 11 12 13 14 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8050
Entity Detected:	Fastkd5, FAST kinase domains 5 ( MGI:2139469)
Sequence:	sense strand is shown >T8050 AAGAGGTAATCCGCTGCCTTCGCGGGCTTCAGTGCTGGAGGAGGCCAGAAGAGGGCGTTGGATCTCCTGG GACTTGAATTAAGGTTGATTGTGAGCCACAGTGTGAGCGCTGGAAATCAAACCCCAATCCTCTGGAAGAG CAGCCAGTACTCTTAAACACTGAGCAATCTCTCCAGCCAGTAGAAGCCACTCATTCTTCATCTACCCTTT TTTCAGTATCTGGCACCAATTCTGGCCCCATCATTTGTGGCCCATGGCTCTTGTGGTATGCCGAAGATTT ACAGGCAGTTTCTGTGGAACATTTCCCTGTCCACCTCTAATGAAGCCCCGTCAAACAAGAACATGTGATT TTGCTCTGACTGCCAAGAAGATCAGCACAGACTCCAGAATGTCAGCCGCTCTCAAGCTATTAGAACCTTT AAAGTACAAAGCACCTTGTAATCCTGCTTACCGTGCAGCCCAAAGCGTGGCCCATTGGCACATGGGAAAC ATCACGCCACACGGGGGACAGACGCTCCCTGAATGTAATAGCTCCTGCCATCTTGCCAGAAAAGTGAAGA ACGTTGGTGGTACCACCCTTCCTCGGAGAACCTTCACAGCCAGCAGTGCCCACCTGGGTTTGGAGTTCAA CAAAGCTTCTACCCTTAATGCCAGCACACTGCATCCAGACTCATCCAGTGCTGGAGGAGGTGAAGAGGAT GTAGAGGTCTTTGATTCCTTCGAAGGAACCCGAGTTTTCTTAAAGCTAAGGCCAGAGTACCAGCTTCACA GCTATAACAGAAGTGACACTCACCAACCCATAGCTGCTTCAGAAGTTGAACTAATTTTGCACAAAGTCAC ATTCTACCAAAATAAACTCCAGCCAGAAGTCATAACTAATTATTTTTATAAGCTCAGCTCTTTGCCAGCA GAACAGAATTCTGTTTTGCTATCCAGTAACAGCTTTGCTTTGCTCTGCCAGCTGAGTGTGAAAAACATAC AGCTCTTTAACACCTCCGATCTGATCAGTATTTTGAAAGCCTTTGTTGACTTAAGAATTCCCCCCTCCCT GTCCATGCTAGATGTGTATGAAACCAGATTTTGCCATCAGGTATGGGAGATGACTCTAGATCAGCTTCTC CTAGTAGCTGATCTTTGGCGGAACTTAGGCCGCAGAGTGCCTCGTTTTTTTAAAATTTTTTTTAGTTATC TTAATTTACACTGGAGAGAGCTATCTTTGTCTCAGCTGATTCACTTAATTTATATTATAGGTGAAAATCG TCAAGTACCACAGGACCTGATGCAAAGACTAGAATCACTGATCCTTAAGTATGTAGATTCAGTCAATTTA GAGGAAGTTGGTACAATCTGTTTGGGGTTTTTTAAATCAAGTAGTAGTCTCTCTGAATTTGTCATGCGGA AAATTGGAGATCTAGCTTGTGCTAACATGCAGCATCTGAGTAGCCACACCTTAGTTCATATTCTTAAAAT GTTCCGCTTCACTCATGTAGATCATATACATTTTATGAAGCAGTTTGGAGAAATTGCTCCTCAGAGAATT CCTTCCTTGGGGGTTCAGGGTGTTATGCACCTCACACTTGCCTGCTCAGCCTTACGCATCCTGGATGAAA GAGTAATGAACGCAGTAGCTGCCTCTTTGCCTCCGAGGGTAGCACACTGTCGAAGTAAAGATGTTGCCAA AATTCTGTGGTCCTTTGGAACTCTGAATTATAAGCCACCCAACACAGAAGAATTTTATTCCAGTCTCATA AATGAAATCCACAGAAAGATGCCTGAATTCAACCAGTACCCAGAGCACCTTCTCACCTGCCTGATTGGCT TGGCATTTTCTGAATACTTTCCAGTTGAGTTCATAAATGTTGCTCTGAGCCCAGGATTTGTCAAGTTAGC TCAAGAGAGAAGTAAGTTTGAACTCACTAAGGAACTGTTTACTCTTGATGGTACAGTTGCCATTGAATGT CCAGATTACAAGGGCAACCGTCTTAATTCTCACCTTCAACAAGAAACATCTGCAAATCTGTGGAATCTAG CAAGTAAGGATATGAGATCAAAGCCTGAATTCCTAGAAACTCTCTATTTACTTGAGACCATGTTGGGTGG CCCCCAGTACATTAAGCACCATATGATTTTGCCTCATACCCGCTCTTCTGACTTAGAGGTTCAGCTTGAT GCTAACATGAAACCAATGCCCTTTAATAGTGAAGCGACACCAACTGAAGATGGAGCTCAATTACGGTTTA AGCAAGTGGGAGTCAGCCTTACAGATGATTTGATGAATCAGTTGCTGAAGGGAAAAGCCAAGAGGTATTT CCAGGGGCAAATTGAGTTAGAGACTGGCCAGCCACCCATGGAGTTAAGAAAGAAGACAACTGTACCCTTG GTGAACTCTGGTTGCAATGGGACAGATAGATTGGGGGATGGGATGGTTGGCTTGTGCCCTCTAGCCCACA TGCAGCCCCCATTAGTGAAGCTAGCTATCCAGTTCACAAACAAGAACCAATACTGCTATGGTTCCAGGGC TCTACTTGGATTGCACAATATGAAGAGGAGGCAGCTAGTTCAAATTGGGTACCGTGTGGTAGAATTACCC CACTGGGAATGGCTTCCACTACTAAAAAGAACTCGCTTAGAGAAGCTAGCATACCTCCATGAGAAAGTGT TCACCTCTGCTCTCTGAAGGGCACTCGTGGACATTTATCCTCATAAAAGGAATAGGTGCATACCTTCTAC CTGTAAGTTGAGCATCCTTAACCAGAAAATCAAAGTCAAATATTAAAAAACCTGGAACTGAGTACTGACA GATGTCAGATCAAGGATATTCAAGTGGTAGGTAGTCTATGTAGATACCCTGGACTTCAAATCTCAAACAC CTCTAGTCTCAAGTATTTGATACCGGGATTCTCAACCTGTCCTCCAAGTAATTATAAAAGCCACAGTGTA GGTGGGTAATAACCGTCTGTTGAGTAGCCATGGGTGCAGTGCCTGTAGCAATTATATATTACGACAGGTT TTGTGTAACCCTGGTTGTACTCTAGTTGTATGTTTTGCTCAGAATAAATAAGCATCTTCAAAATGAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30059761 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30059761 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19523 same embryo
	EMAGE:19526 same embryo
	EMAGE:19522 same embryo
	EMAGE:19524 same embryo
	EurExpress:euxassay_004980 same experiment
	MGI:4824783 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source